Nano-Pulse Stimulation Ablates Orthotopic Rat Hepatocellular Carcinoma and Induces Innate and Adaptive Memory Immune Mechanisms that Prevent Recurrence

Lassiter, Brittany P.; Guo, Siqi; Beebe, Stephen J.

doi:10.3390/cancers10030069

Cited by 29 publications

(8 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although we only examined the single-agent efficacy of NPS on primary tumor elimination in this study, previous published studies have documented the ability of NPS to inhibit both the growth of a tumor cell rechallenge and prevent metastasis in a CD8-dependent manner ( 13 , 15 , 17 , 18 , 34 , 38 ). The RCD process induced by NPS is likely responsible for priming this CD8 + T cell- mediated immune response ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous published work includes treatments of seborrheic keratosis ( 8 ), sebaceous hyperplasia ( 9 ), warts ( 10 ) and basal cell carcinoma ( 11 ). In animal studies, NPS has shown high efficacy in treating a variety of malignant murine tumor types including rat hepatocellular as well as mouse breast, fibrosarcoma, squamous cell carcinoma (SCC), pancreatic, lung and melanoma tumors ( 12 – 18 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nano-pulse stimulation™ therapy (NPS™) is superior to cryoablation in clearing murine melanoma tumors

McDaniel

Freimark²,

Navarro³

et al. 2023

Front. Oncol.

View full text Add to dashboard Cite

BackgroundNano-Pulse Stimulation™ Therapy (NPS™) is a new, bioelectric modality that applies ultrashort pulses of electric energy to trigger regulated cell death in treated tissues. Instead of initiating necrosis by heating or freezing, NPS therapy permeabilizes intracellular organelles to activate the cell’s own self-destruct pathway of programmed or regulated cell death. Unlike cryotherapies that can both damage structural tissues and diffuse into the periphery beyond the margins of the lesion, NPS only affects cells within the treated zone leaving surrounding tissue and acellular components unaffected.MethodsWe generated melanoma tumors in mice by injecting B16-F10 cells intradermally and compared the efficacy and resulting skin damage from Nano-Pulse Stimulation Therapy with that of cryoablation in clearing these tumors.ResultsThe results of the study demonstrate that NPS is superior at clearing B16-F10 melanoma lesions. NPS permanently eliminated up to 91% of all tumor lesions with a single treatment compared to cryoablation that only eliminated up to 66%. Importantly, NPS permanently eliminated these lesions with no recurrence and with minimal dermal fibrosis, underlying muscle atrophy, permanent hair follicle loss or other markers of permanent skin damage.ConclusionsThese findings suggest that NPS is a promising new modality for the clearance of melanoma tumors and is a more efficacious, less damaging approach than cryoablative methods for the treatment of aggressive malignant tumors.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nano-pulse stimulation™ therapy (NPS™) is superior to cryoablation in clearing murine melanoma tumors

McDaniel

Freimark²,

Navarro³

et al. 2023

Front. Oncol.

View full text Add to dashboard Cite

show abstract

“…Nanosecond pulsed electric fields (nsPEFs) can induce cell apoptosis by affecting the plasma membrane and intracellular structure [9,10], inhibiting tumor angiogenesis [11], reducing tumor metastasis [12], and producing antitumor immunity [13,14]. Quite different from irreversible electroporation which generally use parameters of microsecond pulse duration and 1500-V voltage, nsPEFs compressed the pulse width to nanosecond pulse duration under 30000-V voltage.…”

Section: Introductionmentioning

confidence: 99%

Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner

Liu

Dong

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

Background Liver tumor remains an important cause of cancer-related death. Nanosecond pulsed electric fields (nsPEFs) are advantageous in the treatment of melanoma and pancreatic cancer, but their therapeutic application on liver tumors need to be further studied. Methods Hep3B cells were treated with nsPEFs. The biological behaviors of cells were detected by Cell Counting Kit-8, 5-ethynyl-20-deoxyuridine, and transmission electron microscopy (TEM) assays. In vivo, rabbit VX2 liver tumor models were ablated by ultrasound-guided nsPEFs and radiofrequency ablation (RFA). Contrast-enhanced ultrasound (CEUS) was used to evaluate the ablation effect. HE staining and Masson staining were used to evaluate the tissue morphology after ablation. Immunohistochemistry was performed to determine the expression of Ki67, proliferating cell nuclear antigen, and α-smooth muscle actin at different time points after ablation. Results The cell viability of Hep3B cells was continuously lower than that of the control group within 3 days after pulse treatment. The proliferation of Hep3B cells was significantly affected by nsPEFs. TEM showed that Hep3B cells underwent significant morphological changes after pulse treatment. In vivo, CEUS imaging showed that nsPEFs could completely ablate model rabbit VX2 liver tumors. After nsPEFs ablation, the area of tumor fibrosis and the expression of Ki67, proliferating cell nuclear antigen, and α-smooth muscle actin were decreased. However, after RFA, rabbit VX2 liver tumor tissue showed complete necrosis, but the expression of PCNA and α-smooth muscle actin did not decrease compared to the tumor group. Conclusions nsPEFs can induce Hep3B cells apoptosis and ablate rabbit VX2 liver tumors in a non-thermal manner versus RFA. The ultrasound contrast agent can monitor immediate effect of nsPEF ablation. This study provides a basis for the clinical study of nsPEFs ablation of liver cancer.

show abstract

“…Nanosecond pulsed electric fields (nsPEFs) can induce cell apoptosis by affecting the plasma membrane and intracellular structure [9,10], inhibiting tumor angiogenesis [11], reducing tumor metastasis [12], and producing antitumor immunity [13,14]. Quite different from irreversible electroporation which generally use parameters of microsecond pulse duration and 1500-V voltage, nsPEFs use comress the pulse width to nanosecond pusle duration under 30000-V voltage.…”

Section: Introductionmentioning

confidence: 99%

Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner versus radiofrequency ablation

Liu

Sun

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Liver tumor remains an important cause of cancer-related death. Nanosecond pulsed electric fields (nsPEFs) are advantageous in the treatment of melanoma and pancreatic cancer, but their therapeutic application on liver tumors need to be further studied. Methods: Hep3B cells were treated with nsPEFs. The biological behaviors of cells were detected by Cell Counting Kit-8, 5-ethynyl-20-deoxyuridine, and transmission electron microscopy (TEM) assays. In vivo , rabbit VX2 liver tumor models were ablated by ultrasound-guided nsPEFs and radiofrequency ablation (RFA). Contrast-enhanced ultrasound (CEUS) was used to evaluate the ablation effect. HE staining and Masson staining were used to evaluate the tissue morphology after ablation. Immunohistochemistry was performed to determine the expression of Ki67, proliferating cell nuclear antigen, and α-smooth muscle actin at different time points after ablation. Results: The cell viability of Hep3B cells was continuously lower than that of the control group within 3 days after pulse treatment. The proliferation of Hep3B cells was significantly affected by nsPEFs. TEM showed that Hep3B cells underwent significant morphological changes after pulse treatment. In vivo , CEUS imaging showed that nsPEFs could completely ablate model rabbit VX2 liver tumors. After nsPEFs ablation, the area of tumor fibrosis and the expression of Ki67, proliferating cell nuclear antigen, and α-smooth muscle actin were decreased. However, after RFA, rabbit VX2 liver tumor tissue showed complete necrosis, but the expression of PCNA and α-smooth muscle actin did not decrease compared to the tumor group. Conclusions: nsPEFs can induce Hep3B cells apoptosis and ablate rabbit VX2 liver tumors in a non-thermal manner versus RFA. The ultrasound contrast agent can monitor immediate effect of nsPEF ablation. This study provides a basis for the clinical study of nsPEFs ablation of liver cancer.

show abstract

Nano-Pulse Stimulation Ablates Orthotopic Rat Hepatocellular Carcinoma and Induces Innate and Adaptive Memory Immune Mechanisms that Prevent Recurrence

Cited by 29 publications

References 59 publications

Nano-pulse stimulation™ therapy (NPS™) is superior to cryoablation in clearing murine melanoma tumors

Nano-pulse stimulation™ therapy (NPS™) is superior to cryoablation in clearing murine melanoma tumors

Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner

Nanosecond pulsed electric field ablates rabbit VX2 liver tumors in a non-thermal manner versus radiofrequency ablation

Contact Info

Product

Resources

About