Nano-sized metabolic precursors for heterogeneous tumor-targeting strategy using bioorthogonal click chemistry  in vivo

Lee, Sang‐Min; Jung, Seulhee; Koo, Heebeom; Na, Jin Hee; Yoon, Hong Yeol; Shim, Man Kyu; Park, Jooho; Kim, Jong Ho; Lee, Seulki; Pomper, Martin G.; Kwon, Ick Chan; Ahn, Cheol Hee; Kim, Kwangmeyung

doi:10.1016/j.biomaterials.2017.09.025

Cited by 52 publications

(37 citation statements)

References 48 publications

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“…In this way, genetically different and heterogeneous tumor cells can be converted into phenotypically uniform cells, leading to a uniform EPR effect for subsequently administered nanomedicine ( Figure 6A ) 97, 98. Previous studies have shown that an artificial azide reporter, originating from the metabolic precursor, tetraacetylated N-azidoacetyl-D-mannosamine (Ac 4 ManNAz) can be presented on the surface of tumor cells by metabolic glycoengineering ( Figure 6B ) 99, 100. Subsequently, Chlorin e6 (Ce6)-containing NPs which are decorated with the ligands, bicycle[6.1.0]nonyne to bind to artificial receptors were administered and bound to artificial receptors on tumors through bioorthogonal click reaction between azide groups and the ligands, leading to photodynamic therapy in vivo regardless of tumor types ( Figure 6C ) 98.…”

Section: Physiological Remodeling Of Tmementioning

confidence: 99%

Alliance with EPR Effect: Combined Strategies to Improve the EPR Effect in the Tumor Microenvironment

et al. 2019

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The use of nanomedicine for cancer treatment takes advantage of its preferential accumulation in tumors owing to the enhanced permeability and retention (EPR) effect. The development of cancer nanomedicine has promised highly effective treatment options unprecedented by standard therapeutics. However, the therapeutic efficacy of passively targeted nanomedicine is not always satisfactory because it is largely influenced by the heterogeneity of the intensity of the EPR effect exhibited within a tumor, at different stages of a tumor, and among individual tumors. In addition, limited data on EPR effectiveness in human hinders further clinical translation of nanomedicine. This unsatisfactory therapeutic outcome in mice and humans necessitates novel approaches to improve the EPR effect. This review focuses on current attempts at overcoming the limitations of traditional EPR-dependent nanomedicine by incorporating supplementary strategies, such as additional molecular targeting, physical alteration, or physiological remodeling of the tumor microenvironment. This review will provide valuable insight to researchers who seek to overcome the limitations of relying on the EPR effect alone in cancer nanomedicine and go “beyond the EPR effect”.

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Section: Physiological Remodeling Of Tmementioning

confidence: 99%

Alliance with EPR Effect: Combined Strategies to Improve the EPR Effect in the Tumor Microenvironment

et al. 2019

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“…Glycoengineering, a technique that allows manipulation of cellular membrane glycans to modify cells, provides an intriguing method to homogenously regulate paracrine properties at a cellular level. [ 21–24 ]…”

Section: Introductionmentioning

confidence: 99%

Single‐Cell Encapsulation via Click‐Chemistry Alters Production of Paracrine Factors from Neural Progenitor Cells

Swaminathan

Malkovskiy

et al. 2020

Advanced Science

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Extracellular matrix (ECM) properties affect multiple cellular processes such as cell survival, proliferation, and protein synthesis. Thus, a polymeric‐cell delivery system with the ability to manipulate the extracellular environment can act as a fundamental regulator of cell function. Given the promise of stem cell therapeutics, a method to uniformly enhance stem cell function, in particular trophic factor release, can prove transformative in improving efficacy and increasing feasibility by reducing the total number of cells required. Herein, a click‐chemistry powered 3D, single‐cell encapsulation method aimed at synthesizing a polymeric coating with the optimal thickness around neural progenitor cells is introduced. Polymer encapsulation of neural stem cells significantly increases the release of neurotrophic factors such as VEGF and CNTF. Cell encapsulation with a soft extracellular polymer upregulates the ADCY8‐cAMP pathway, suggesting a mechanism for the increase in paracrine factors. Hence, the described single‐cell encapsulation technique can emerge as a translatable, nonviral cell modulation method and has the potential to improve stem cells' therapeutic effect.

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“…Moreover, Lee et al developed nano-sized metabolic precursors (Nano-MPs) with a combined structure of generation 4 poly(amidoamine) dendrimer back bone and triacetylated N -azidoacetyl- d -mannosamine. The intravenous injection of Nano-MPs in tumor-bearing mice showed the localization of azide groups in tumor tissues, indicating that Ac 4 ManNAz was specifically delivered to tumor tissues within which azide groups were successfully generated [37]. As such, nano-sized carriers loaded or conjugated with Ac 4 ManNAz have been demonstrated to introduce sufficient amounts of azide groups into tumor cells to improve the accumulation of click chemistry chemical-drug conjugates.…”

Section: Applications Of Click Chemistry For Drug Delivery In the mentioning

confidence: 99%

“…Researchers have recently reported that modifying nanoparticles with click chemistry chemicals such as BCN and DBCO improves the affinity between nanoparticles and azide-labeled cancer cells. Lee et al demonstrated that tumor targeting using two types of nanoparticles improved the accumulation of drug-containing nanoparticles in tumors [37]. First, they synthesized CNPs conjugated with BCN and chlorine e6 (BCN-Ce6-CNPs).…”

Section: Applications Of Click Chemistry For Drug Delivery In the mentioning

confidence: 99%

Click Chemistry as a Tool for Cell Engineering and Drug Delivery

2019

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Click chemistry has great potential for use in binding between nucleic acids, lipids, proteins, and other molecules, and has been used in many research fields because of its beneficial characteristics, including high yield, high specificity, and simplicity. The recent development of copper-free and less cytotoxic click chemistry reactions has allowed for the application of click chemistry to the field of medicine. Moreover, metabolic glycoengineering allows for the direct modification of living cells with substrates for click chemistry either in vitro or in vivo. As such, click chemistry has become a powerful tool for cell transplantation and drug delivery. In this review, we describe some applications of click chemistry for cell engineering in cell transplantation and for drug delivery in the diagnosis and treatment of diseases.

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Nano-sized metabolic precursors for heterogeneous tumor-targeting strategy using bioorthogonal click chemistry in vivo

Cited by 52 publications

References 48 publications

Alliance with EPR Effect: Combined Strategies to Improve the EPR Effect in the Tumor Microenvironment

Alliance with EPR Effect: Combined Strategies to Improve the EPR Effect in the Tumor Microenvironment

Single‐Cell Encapsulation via Click‐Chemistry Alters Production of Paracrine Factors from Neural Progenitor Cells

Click Chemistry as a Tool for Cell Engineering and Drug Delivery

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