Nano structures via laser interference patterning for guided cell growth of neuronal cells

Bremus-Koebberling, Elke A.; Beckemper, Stefan; Koch, Beate; Gillner, Arnold

doi:10.2351/1.4730804

Cited by 46 publications

(32 citation statements)

References 22 publications

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“…In vitro results, showed a better cell alignment for patterns formed with lines separated distances below 50 µm. Bremus-Koebberling et al [45] followed a similar approach, but using nano-grooves. They evaluated the influence of linear-like nanopatterns produced by laser interference patterning (λ = 355 nm, pulse duration = 38 ns) on the cell alignment using B35 neuronal cells.…”

Section: Methodsmentioning

confidence: 99%

Laser Surface Texturing of Polymers for Biomedical Applications

et al. 2018

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Polymers are materials widely used in biomedical science because of their biocompatibility, and good mechanical properties (which, in some cases, are similar to those of human tissues); however, these materials are, in general, chemically and biologically inert. Surface characteristics, such as topography (at the macro-, micro, and nano-scale), surface chemistry, surface energy, charge, or wettability are interrelated properties, and they cooperatively influence the biological performance of materials when used for biomedical applications. They regulate the biological response at the implant/tissue interface (e.g., influencing the cell adhesion, cell orientation, cell motility, etc.). Several surface processing techniques have been explored to modulate these properties for biomedical applications. Despite their potentials, these methods have limitations that prevent their applicability. In this regard, laser-based methods, in particular laser surface texturing (LST), can be an interesting alternative. Different works have showed the potentiality of this technique to control the surface properties of biomedical polymers and enhance their biological performance; however, more research is needed to obtain the desired biological response. This work provides a general overview of the basics and applications of LST for the surface modification of polymers currently used in the clinical practice (e.g., PEEK, UHMWPE, PP, etc.). The modification of roughness, wettability, and their impact on the biological response is addressed to offer new insights on the surface modification of biomedical polymers.

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Section: Methodsmentioning

confidence: 99%

Laser Surface Texturing of Polymers for Biomedical Applications

et al. 2018

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“…[2][3][4] Researchers have been working on micro-or nanopatterned substrates to guide neuronal outgrowth, to help study the neural electrical signal transmission in single neurons, 5 and to aid nerve regeneration processes. 6 Therefore, our goal is to combine microfluidics with tissue engineering to create a "living brain," generating realistic in vitro neural circuitry, which can be used to standardize experimental neuronal cell culture. 7 Networks of neurons cultured on-chip can provide insights into both normal and disease-state brain function.…”

Section: Introductionmentioning

confidence: 99%

Nanoscaffold's stiffness affects primary cortical cell network formation

Xie

Schurink

Wolbers

et al. 2014

Journal of Vacuum Science &Amp; Technology B, Nanotechnology and Microelectronics: Materials, Processing, Measurement, and Phen

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Networks of neurons cultured on-chip can provide insights into both normal and disease-state brain function. The ability to guide neuronal growth in specific, artificially designed patterns allows us to study how brain function follows form. Primary cortical cells cultured on nanograting scaffolds, in particular astrocytes, showed highly ordered regions of dendritic outgrowth. Usually, materials suitable for nanopatterning have a stiffness far above that of the extracellular matrix. In this paper, the authors studied two materials with large differences in stiffness, polydimethylsiloxane (PDMS) and silicon. Our results show that both nanopatterned silicon and PDMS guide the outgrowth of astrocytes in cortical cell culture, but the growth of the astrocyte is affected by the stiffness of the substrate, as revealed by differences in the cell soma size and the organization of the outgrowth.

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“…20 Bremus-Koebberling et al proposed a conduit with various combinational patterns of groove width and depth and reported that different width and depth combinations had different guiding capabilities. 21 Bechara and Popat observed the attachment and growth of neural progenitor cells on a scaffold with micropatterns and nanowires. Their experimental results indicated that micro/ nanohybrid structures could affect the morphology of cells and promote cell differentiation.…”

Section: Introductionmentioning

confidence: 99%