Nano‐textured fluidic biochip as biological filter for selective survival of neuronal cells

Abstract

This is an innovative study to engineer biological filter to evaluate the effect of template surface structure and physiochemical properties that can be used for wide variety of applications in biological, health care as well as environmental protection. Specifically, planar silicon (Si) wafer and arrayed Si nano-tips (SiNT) templates were fabricated and coated with gold for various lengths of time to study the effect of surface charge, surface roughness, and hydrophilicity on biological activity of rat pheochromocytoma cell lines PC12. The initial growth and proliferation of PC12 cells on Si and SiNT templates showed an antipathy for the ultra-sharp SiNTs templates. In contrast, the same cells demonstrated a preferable adherence to and proliferation on planar Si templates, resulting in higher cell densities by three orders of magnitude than those on SiNT templates. It is hypothesized that SiNTs array does generate nano-fluidic effect such that the effective contact region for aqueous solution on SiNTs is lower than that on planar Si templates, thus decreasing adsorbable area for cell viability and survival. Moreover, the effect of the gold coating on cell number density was analyzed in terms of the surface roughness, zeta potential and wetting properties of the templates. It was determined that surface charge, as measured by the zeta potential, strongly correlated with the trend observed in the surface cell density, whereas no such correlation was observed for surface roughness or wetting properties in the ranges of our experiment conditions.

Neurogenesis-on-Chip: Electric field modulated transdifferentiation of human mesenchymal stem cell and mouse muscle precursor cell coculture

“…Yamada et al reported that mild electrical stimulation strongly influences embryonic stem cells to assume neuronal fate [11]. Han et al influences the cell more than the wettability and roughness of the substrate [12]. As an extension of their observation, the researchers have acknowledged that the differentiation of stem cells under the influence of external cues can open up possibilities for studying novel mechanisms underlying cellular differentiation and more importantly, suggested the possibilities for adaption in clinical contexts.…”

Design and Applications of Cell-Selective Surfaces and Interfaces

“…A separation of ∼2 μm between cell-repulsive PEG hydrogels is enough to guide neurite growth and inhibit astrocyte adhesion and growth . Nanostructure and nanofluidic transport used by Han et al selectively enhance survival of neuronal cells . Human embryonic stem cells (hESCs) are differentiated into neurons by ridge/groove patterns .…”

Early Osteogenic Marker Expression in hMSCs Cultured onto Acid Etching-Derived Micro- and Nanotopography 3D-Printed Titanium Surfaces

“…For these reasons, researchers are driven to find and design synthetic and more feasible solutions, such the development of polymeric biomaterials, 3D-bone implants and biodegradable devices [5][6][7][8]. Furthermore, great attention continues to be directed to the development of new technologies, such as surface modification techniques, to increase the actual clinical performance of implant devices [9,10]. Orthopaedic biomaterials are designed to be implanted in the human body as components of devices for replacing or repairing various tissues such as bone, cartilage or ligaments and tendons, and even to direct and guide bone restoration when necessary.…”