Nano zero-valent iron harms methanogenic archaea by interfering with energy conservation and methanogenesis

Abstract

Iron-based nanoparticles (Fe-NPs), often applied for pollutant remediation, inevitably interact with various organisms either directly or indirectly. The well-acknowledged toxicological mechanisms for Fe-NPs on organisms are causing oxidative damage and...

Escalating Photobiological Hydrogen Production Using Engineered Selenium Nanoparticles

“…Oxidative stress caused by cellular internalization of NPs is a well-recognized mechanism that inhibits the physiological growth of microorganisms, and an evaluation of the toxicity of SeNPs and the decorated SeNPs was therefore conducted by monitoring the abundance of intracellular ROS . Amendment of cultures with SeNPs at increasing concentrations produced increasing generation of ROS ( p < 0.05 or p < 0.01) (Figure S4a), which is consistent with results from previous reports where internalized NPs induced oxidative stress. , In addition, Se is required in the active center of GSH peroxidase, which catalyzes the oxidation of GSH to glutathione disulfide for scavenging ROS, and the intracellular GSH abundance was therefore evaluated. An increase in the concentration of added SeNPs or decorated SeNPs produced a decrease in the abundance of GSH (Figure S4b), indicating that antioxidative activity was enhanced with SeNP amendment.…”

“…43 Amendment of cultures with SeNPs at increasing concentrations produced increasing generation of ROS (p < 0.05 or p < 0.01) (Figure S4a), which is consistent with results from previous reports where internalized NPs induced oxidative stress. 44,45 In addition, Se is required in the active center of GSH peroxidase, which catalyzes the oxidation of GSH to glutathione disulfide for scavenging ROS, and the intracellular GSH abundance was The number of amino acids identified as a proportion of the total number of amino acids in the protein. b The number of peptides used for quality.…”

Unraveling quinoproteins-based extracellular electron transport in humus-dependent respiratory growth of Methanosarcina acetivorans

“…Organism and growth conditions M. acetivorans C2A was purchased from the Japan Collection of Microorganisms (JCM, Japan) and was cultivated under strict anaerobic conditions at 37°C in a high-salt medium, as previously reported 39 . Methanol that was passed through a 0.22 mm lter was added to provide a nal concentration of 125 mM and was used as the methanol-grown substrate.…”

“…The production of methane and total cellular protein concentrations were measured as previously reported 39 . The methanol concentrations were measured by a gas chromatograph (GC-2014, Shimadzu, Japan) equipped with a DB-FFAP column (internal diameter, 0.32 mm; length, 30 m) and a ame ionization detector.…”

“…A total of 10 mL of cells that were cultured at the mid-log phase were harvested by centrifugation at 5,000×g for 10 min. The supernatant was discarded, and the cell pellet was washed twice and analyzed by SEM and FTIR as described elsewhere 39 .…”