Nano zinc oxide-functionalized nanofibrous microspheres: A bioactive hybrid platform with antimicrobial, regenerative and hemostatic activities

Metwally, Walaa; El-Habashy, Salma E.; El-Nikhely, Nefertiti; Mahmoud, Hoda; Eltaher, Hoda M.; El-Khordagui, Labiba K.

doi:10.1016/j.ijpharm.2023.122920

Cited by 13 publications

(15 citation statements)

References 80 publications

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“…Blank PLA NF MS were produced using a thermally induced phase separation technique [22]. In brief, a PLA solution (2% w/v) in THF was homogenized with glycerol in an organic: aqueous phase ratio of 1:3 at 6000 rpm for 5 min at 50 • C. The mixture was then quenched in liquid nitrogen and solvent exchange was allowed using ice-cold deionized water at 4 • C for 24 h. NF MS bioactivated with ZNP (Z-MS) or D-ZNP (DZ-MS) were synthesized via uniform dispersion of ZNP or D-ZNP (1.5% DDAB) in the PLA solution (2% w/v) in THF at 50 • C to achieve a final NP concentration of 50% w/v prior to MS formation.…”

Section: Development and Characterization Of Blank And Nf 3d Printabl...mentioning

confidence: 99%

“…In our earlier work [22], we prepared Z-MS and DZ-MS as nano/microparticulate hybrid frameworks (average size of 22.2 ± 2.26 µm and 75% NP entrapment efficiency) exclusively comprising interconnecting PLA nanofibers. The MS matrix suppressed NP concentration-dependent cytotoxicity as demonstrated by cell culture studies and allowed sustained release of Zn 2+ from both ZNP and D-ZNP.…”

Section: Composite 3d-printable Inks 3121 Formulationmentioning

confidence: 99%

“…Clinically, composite ZNP/calcium alginate dressings were shown to improve tissue regeneration in diabetic patients while avoiding complications of type 2 diabetes [21]. The antibacterial mechanism of ZNP potentially involves electrostatic interaction with bacterial cell membrane, release of Zn 2+ ions and generation of reactive oxygen species [22]. In addition, ZNP exert an antioxidant/anti-inflammatory effect and enhance the migration of keratinocytes and epithelial cells, speeding wound repair [23].…”

Section: Introductionmentioning

confidence: 99%

“…The scaffold presented an innovative bioinspired bilayer hydrogel structure, with a relatively dense plain HA/CS epidermis-mimicking protective layer topping a more porous bioactive antimicrobial/regenerative dermis-mimicking layer of the same hydrogel. The bottom layer was bioactivated utilizing the injectable NF inorganic/organic hybrid microconstructs recently developed by our team [22]. The microconstructs comprise NF PLA microspheres (MS) incorporating zinc oxide NPs (ZNP) or DDABtreated ZNP (D-ZNP) as single multifunctional bioactive MS (ZNP@MS or D-ZNP@MS).…”

Section: Introductionmentioning

confidence: 99%

“…Secondly, although different ZNP-based film, hydrogel, electrospun formulations have been reported [19,20], the wound healing efficacy of ZNP-bioactivated 3D printed scaffolds has not been documented to date. Thirdly, the in vivo wound healing performance of D-ZNP has not been evaluated despite remarkable in vitro antibacterial activity [34] and highly promising in vitro wound healing aptitudes and hemostatic potential [22]. Formulation and characterization of plain and composite HA/CS-based inks were performed for the fabrication of bioinspired bilayer scaffolds with customizable chemical composition and porosity.…”

Section: Introductionmentioning

confidence: 99%

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Bioinspired 3D-printed scaffold embedding DDAB-nano ZnO/nanofibrous microspheres for regenerative diabetic wound healing

Metwally,

El-Habashy,

El-Hosseiny

et al. 2023

Biofabrication

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There is a constant demand for novel materials/biomedical devices to accelerate the healing of hard-to-heal wounds. Herein, an innovative 3D-printed bioinspired construct was developed as an antibacterial/regenerative scaffold for diabetic wound healing. Hyaluronic/chitosan (HA/CS) ink was used to fabricate a bilayer scaffold comprising a dense plain hydrogel layer topping an antibacterial/regenerative nanofibrous layer obtained by incorporating the hydrogel with polylactic acid nanofibrous microspheres (MS). These were embedded with nano ZnO (ZNP) or didecyldimethylammonium bromide (DDAB)-treated ZNP (D-ZNP) to generate the antibacterial/healing nano/micro hybrid biomaterials, Z-MS@scaffold and DZ-MS@scaffold. Plain and composite scaffolds incorporating blank MS (blank MS@scaffold) or MS-free ZNP@scaffold and D-ZNP@scaffold were used for comparison. 3D printed bilayer constructs with customizable porosity were obtained as verified by SEM. The DZ-MS@scaffold exhibited the largest total pore area as well as the highest water-uptake capacity and in vitro antibacterial activity. Treatment of Staphylococcus aureus-infected full thickness diabetic wounds in rats indicated superiority of DZ-MS@scaffold as evidenced by multiple assessments. The scaffold afforded 95% wound-closure, infection suppression, effective regulation of healing-associated biomarkers as well as regeneration of skin structure in 14 d. On the other hand, healing of non-diabetic acute wounds was effectively accelerated by the simpler less porous Z-MS@scaffold. Information is provided for the first-time on the 3D printing of nanofibrous scaffolds using non-electrospun injectable bioactive nano/micro particulate constructs, an innovative ZNP-functionalized 3D-printed formulation and the distinct bioactivity of D-ZNP as a powerful antibacterial/wound healing promotor. In addition, findings underscored the crucial role of nanofibrous-MS carrier in enhancing the physicochemical, antibacterial, and wound regenerative properties of DDAB-nano ZnO. In conclusion, innovative 3D-printed DZ-MS@scaffold merging the MS-boosted multiple functionalities of ZNP and DDAB, the structural characteristics of nanofibrous MS in addition to those of the 3D-printed bilayer scaffold, provide a versatile bioactive material platform for diabetic wound healing and other biomedical applications.

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Section: Development and Characterization Of Blank And Nf 3d Printabl...mentioning

confidence: 99%

Section: Composite 3d-printable Inks 3121 Formulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioinspired 3D-printed scaffold embedding DDAB-nano ZnO/nanofibrous microspheres for regenerative diabetic wound healing

Metwally,

El-Habashy,

El-Hosseiny

et al. 2023

Biofabrication

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Nanofiber‐based Multifunctional Microspheres for Rapid Hemostasis and Microorganism Removal of Water

Li,

Ye,

Leng

et al. 2024

Adv Healthcare Materials

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Constructing hemostats capable of effectively controlling severe hemorrhage from irregular wounds presents significant challenges and imperatives. In this study, a novel approach is introduced using nanofibrous chitin microspheres (NCM) that are compressed to 60% strain (NCM‐60%) to amplify their water‐initiated expansion performance. This unique capacity allows NCM‐60% to efficiently conform to and fill irregular bleeding cavities, even those of varying depths and curvatures, thereby promoting rapid blood coagulation at deep hemorrhage sites. NCM‐60% exhibits effective control of severe femoral artery and “J”‐shaped liver hemorrhages in 151 ± 6 s and 68 ± 15 s, respectively, revealing its exceptional hemostatic efficacy. Furthermore, NCM‐60% exhibited promising capabilities in removing microbes from water, achieving removal rates of over 96% of bacteria. Blood compatibility assessments and cytotoxicity tests further confirmed the favorable biocompatibility of NCM‐60%. Importantly, NCM‐60% is found to biodegrade and be absorbed in vivo within 12 weeks. This study represents the first instance of leveraging chitin nanofiber‐based biomaterials to design water‐initiated expansion micro‐hemostat, and integrate hemostatic functions with waterborne microorganism removal, thereby expanding the potential applications of micro‐nanostructural materials in emergency first‐aid scenarios.

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Emerging materials and technologies for advancing bioresorbable surgical meshes

Shiroud Heidari,

Dodda,

El-Khordagui

et al. 2024

Acta Biomaterialia

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Nano zinc oxide-functionalized nanofibrous microspheres: A bioactive hybrid platform with antimicrobial, regenerative and hemostatic activities

Cited by 13 publications

References 80 publications

Bioinspired 3D-printed scaffold embedding DDAB-nano ZnO/nanofibrous microspheres for regenerative diabetic wound healing

Bioinspired 3D-printed scaffold embedding DDAB-nano ZnO/nanofibrous microspheres for regenerative diabetic wound healing

Nanofiber‐based Multifunctional Microspheres for Rapid Hemostasis and Microorganism Removal of Water

Emerging materials and technologies for advancing bioresorbable surgical meshes

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