Nano β-tricalcium phosphate/hydrogel encapsulated scaffolds promote osteogenic differentiation of bone marrow stromal cells through ATP metabolism

Zhuang, Pengzhen; Wu, Xiaopei; Dai, Hongyue; Yao, Yue; Qiu, Tong; Han, Yingchao; Li, Shipu

doi:10.1016/j.matdes.2021.109881

Cited by 19 publications

(18 citation statements)

References 44 publications

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“…Here, we have studied the potential of Mn-doped BCP in combination with a hydrogel system, and the advantage of this formulation showed a positive impact on printability parameters as well as the fate determination of hUMSCs. Researchers have widely investigated the incorporation of nanomaterials into scaffolds to overcome challenges associated with polymers. ,, Successfully synthesized Mn-doped BCP was mixed with different w/v % of GelMa and alginate to find the desired concentration with the extrudability test. Figure a demonstrated that the viscosity of the GelMa and alginate was enhanced by the addition of Mn-doped BCP.…”

Section: Discussionmentioning

confidence: 99%

“…Gelatin methacrylate is a biocompatible, naturally derived polymer with the advantage of photo-cross-linking in the presence of a lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) photoinitiator. , BCP influences the degradability and regeneration capabilities based on the calcium-to-phosphate ratio . Biphasic calcium phosphate consists of hydroxyapatite and β-tricalcium phosphate, which significantly impacted bone tissue regeneration as per the reported literature. ,, Doping with trace elements gained popularity to stabilize the phase of ceramic structures as well as enhance the bioactivity of bulk materials. − Addition of manganese (Mn) doping has previously exhibited a positive impact on stem cell differentiation into specific lineages based on doping concentration. , …”

Section: Introductionmentioning

confidence: 99%

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Biomimetic Mineralization of Mn-Doped Biphasic Calcium Phosphate in the GelMa Hydrogel Acting as a Functional 3D Bioscaffold for Osteo Defect Repair

Kamaraj,

Datla,

Moulton

et al. 2023

ACS Appl. Polym. Mater.

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Multicomponent composite scaffolds are stimulating biomaterials that contribute to enhanced physical and mechanical properties and are able to induce or enhance cell differentiation toward specific lineages depending upon their composition. In the case of bone tissue engineering, ceramic components act as "osteoinductive", which, when incorporated into a polymer system, helps encapsulated cells undergo osteodifferentiation. However, formulating a tissue-personalized bioink to provide an appropriate cell niche is challenging. The reproducibility of the bioink and its in vitro characterization are crucial for a scaffold. Here, we show that the composite scaffold drives the human umbilical cord mesenchymal stem cells (hUMSCs) into osteogenic lineage without the addition of any growth factors. Our findings demonstrate that incorporation of Mn-doped BCP into the hydrogel system consisting of gelatin methacrylate and sodium alginate improved the printability and mechanical strength of the scaffold. The possible mechanism behind the enhancement in physical and mechanical properties of bioink is intermolecular ionic interaction and hydrogen bonding between the polymer and bioceramic. Biological results indicate that the composite scaffolds are biocompatible and support cell proliferation during the course of cell culture. Furthermore, RT-qPCR, alkaline phosphate, and alizarin red staining were also used to assess the differentiation ability of cells. The results show that Mn-doped BCP significantly accelerated the osteogenic differentiation of hUMSCs encapsulated within the composite scaffolds. Overall, these results validate the use of Mn-doped BCP bioink for osteo defect regeneration in vitro and provide intriguing opportunities for mineralized bone scaffolds to advance in bone tissue engineering using 3D bioprinting.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biomimetic Mineralization of Mn-Doped Biphasic Calcium Phosphate in the GelMa Hydrogel Acting as a Functional 3D Bioscaffold for Osteo Defect Repair

Kamaraj,

Datla,

Moulton

et al. 2023

ACS Appl. Polym. Mater.

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“…Nrf2/Kelch has also been shown to be involved in ROS production, and NDUFA is differentially expressed in bone microenvironment expression (Gao et al, 2020). Right now, many studies have shown that mitochondrial‐related functions have favorable preventive and therapeutic effects on osteoporosis (Feng et al, 2010; Li et al, 2021; Mackinnon et al, 2011; Nojiri et al, 2011; Vervoort et al, 1997; Zhuang et al, 2021; Zinnuroglu et al, 2012). The differentiation process of osteoblasts in vitro is accompanied by changes in intracellular ATP production, energy metabolism and mitochondrial membrane potential (Komarova et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

TMT‐based proteomics analysis to screen potential biomarkers of Achyranthis Bidentatae Radix for osteoporosis in rats

Yang

Hou

Zhang

et al. 2022

Biomedical Chromatography

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This study aimed to explore the possible mechanism of Achyranthis Bidentatae Radix for the treatment of osteoporosis using tandem mass tag-based proteomics technique combined with mass spectrometry. Proteomics techniques combined with bioinformatics were used to analyze the biological functions of differentially expressed proteins. In addition, western blotting was performed to verify the expression of related proteins. A total of 3,752 proteins were identifiable by proteomic analysis. Furthermore, 93 differentially expressed proteins were identified, of which 61 were upregulated and 32 were downregulated. Differentially expressed proteins were primarily associated with oxidative phosphorylation (p = 4.8 Â 10 À4 ) pathways and involved in transmembrane transport (p = 3.5 Â 10 À3 ), exocytic process (p = 1.2 Â 10 À2 ), cellular developmental process (p = 1.3 Â 10 À2 ), adenosine triphosphate metabolic process (p = 1.0 Â 10 À2 ) and other biological processes. Western blotting analysis showed that MT-CYB and NDUFA9 were differentially expressed in the bone microenvironment of rats with osteoporosis. We speculated that they were potential biomarkers linked to osteoporosis. This study employed proteomics to explore the potential therapeutic targets of Achyranthis Bidentatae Radix to treat osteoporosis. This revealed that mitochondria are a new target for the treatment of glucocorticoid-induced osteoporosis.

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“…[15] We studied the fluidity, compressive strength, and swelling ratios of hydrogels with various SAMA/GelMA compositions in order to figure out the most appropriate one for constructing the osteochondral scaffolds (the SAMA:GelMA ratios were 1.0%:4.0%, 1.5%:3.5%, 2.0%:3.0%, and 2.5%:2.5% (w/w) for CLH1/OLH1, CLH2/OLH2, CLH3/OLH3, and CLH4/OLH4, respectively). To introduce calcium ions in the environment for osteogenesis, the inorganic 𝛽-TCP nanoparticles (162 nm) [16] were incorporated into the bone-regenerating layer. As the results shown in Table S2, Supporting Information, the fluidity of the precursor solution for chondral layer hydrogel chondral layer hydrogel (CLH) decreased as the content of SAMA increased from 1.0% (w/w) to 2.5% (w/w), which could be understood by the high viscosity of alginate solution.…”

Section: Synthesis and Characterization Of Sama/gelma Hydrogelsmentioning

confidence: 99%

A One‐Stone‐Two‐Birds Strategy for Osteochondral Regeneration Based on a 3D Printable Biomimetic Scaffold with Kartogenin Biochemical Stimuli Gradient

Wei

Liu

Kang

et al. 2023

Adv Healthcare Materials

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Osteochondral defect (OCD) regeneration remains challenging because of the hierarchy of the native tissue including both the articular cartilage and the subchondral bone. Constructing an osteochondral scaffold with biomimetic composition, structure, and biological functionality is the key to achieve its high-quality repair. In the present study, an injectable and 3D printable bilayered osteochondral hydrogel based on compositional gradient of methacrylated sodium alginate, gelatin methacryloyl, and 𝜷-tricalcium phosphate (𝜷-TCP), as well as the biochemical gradient of kartogenin (KGN) in the two well-integrated zones of chondral layer hydrogel (CLH) and osseous layer hydrogel (OLH) is developed. In vitro and subcutaneous in vivo evaluations reveal that apart from the chondrogenesis of the embedded bone mesenchymal stem cells induced by CLH with a high concentration of KGN, a low concentration of KGN with 𝜷-TCP in the OLH synergistically achieves superior osteogenic differentiation by endochondral ossification, instead of the intramembranous ossification using OLH with only 𝜷-TCP. The biomimetic construct leveraging KGN as the only biochemical inducer can facilitate cartilage and subchondral bone restoration in the in vivo osteochondral defect. This one-stone-two-birds strategy opens up a new facile approach for OCD regeneration by exploiting the biological functions of the bioactive drug molecule KGN.

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Nano β-tricalcium phosphate/hydrogel encapsulated scaffolds promote osteogenic differentiation of bone marrow stromal cells through ATP metabolism

Cited by 19 publications

References 44 publications

Biomimetic Mineralization of Mn-Doped Biphasic Calcium Phosphate in the GelMa Hydrogel Acting as a Functional 3D Bioscaffold for Osteo Defect Repair

Biomimetic Mineralization of Mn-Doped Biphasic Calcium Phosphate in the GelMa Hydrogel Acting as a Functional 3D Bioscaffold for Osteo Defect Repair

TMT‐based proteomics analysis to screen potential biomarkers of Achyranthis Bidentatae Radix for osteoporosis in rats

A One‐Stone‐Two‐Birds Strategy for Osteochondral Regeneration Based on a 3D Printable Biomimetic Scaffold with Kartogenin Biochemical Stimuli Gradient

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