Nanoarray‐Surfaces by Reconstitution of the Porin MspA into Stabilized Long‐Chain‐Lipid‐Monolayers at a Gold‐Surface

Wörner, Michael; Niebler, Sandra; Gogritchiani, Eliso; Egner, Nicole; Braun, André M.; Niederweis, Michael; Bossmann, Stefan H.

doi:10.1002/elan.200603636

Cited by 8 publications

(10 citation statements)

References 54 publications

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“…REM of an A 3 SAM on gold in the presence and absence of reconstituted MspA has been reported in an earlier paper 33. However, at that time only images with a considerably low resolution were available.…”

Section: Resultsmentioning

confidence: 76%

“…Note that the electrodes were thoroughly rinsed with ultrapure water between all of the steps described above. Our methodology for recording and interpreting electrochemical impedance spectra has been described in detail in an earlier paper 33. The electrochemical impedance spectra were recorded at −0.4 V (corresponding to a cathodic polarization of 450 to 500 mV with respect to the open‐circuit potential) in order to support the permeation of water and solvated ions through the SAM (see Figure 6).…”

Section: Resultsmentioning

confidence: 99%

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Characterization of Nanostructured Surfaces Generated by Reconstitution of the Porin MspA from Mycobacterium smegmatis

Wörner

Lioubashevski

Basel

et al. 2007

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Nanostructures with long-term stability at the surface of gold electrodes are generated by reconstituting the porin MspA from Mycobacterium smegmatis into a specially designed monolayer of long-chain lipid surfactant on gold. Tailored surface coverage of gold electrodes with long-chain surfactants is achieved by electrochemically assisted deposition of organic thiosulfates (Bunte salts). The subsequent reconstitution of the octameric-pore MspA is guided by its extraordinary self-assembling properties. Importantly, electrochemical reduction of copper(II) yields copper nanoparticles within the MspA nanopores. Electrochemical impedance spectroscopy, reflection electron microscopy, and atomic force microscopy (AFM) show that: 1) the MspA pores within the self-assembled monolayer (SAM) are monodisperse and electrochemically active, 2) MspA reconstitutes in SAMs and with a 10-nm thickness, 3) AFM is a suitable method to detect pores within SAMs, and 4) the electrochemical reduction of Cu2+ to Cu0 under overpotential conditions starts within the MspA pores.

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Section: Resultsmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Characterization of Nanostructured Surfaces Generated by Reconstitution of the Porin MspA from Mycobacterium smegmatis

Wörner

Lioubashevski

Basel

et al. 2007

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“…Sulfur-centered radicals are intrinsically more stable 16,17 and prone to interact with metal surfaces. [18][19][20][21] In order to prove this hypothesis, spectroelectrochemical experiments were performed with DFTBut in the absence of Au, Ag or Pd ions. As can be seen in figure 2 the differential spectra recorded at different applied potentials only show a decrease of the DTFBut concentration on the electrode surface (decrease in the absorption band at 454 nm) leading to the formation of oxidized species, DTF ox , which show absorption bands at around 400 and 485 nm.…”

Section: Resultsmentioning

confidence: 99%

Remarkable effect of the dithiafulvene structures on their capacity as reducing agents: Influence of conjugated thiocarbonyl group

Lanterna

Torresán

Iglesias

et al. 2019

Applied Surface Science

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The use of dithiafulvene (DTF) derivative compounds having strong electron donating properties as reducing agents to obtain noble metal nanoparticles (PdNP, AgNP, AuNP) is demonstrated. The resulting oxidized DTF product was characterized by spectroelectrochemistry. The stability of the colloidal dispersions are explained considering that the oxidized DTF layer have a delocalized positive charge providing both steric and electrostatic stabilization, which allows that the generated nanoparticles remain in a colloidal state for six months or more. Our results suggest that the presence of a thiocarbonyl group in DTF moeity increases the stability of the oxidized DTF (radical cation DTF 2 +• ), avoiding the formation of the tetrathiafulvene (TTF) as occurs with other derivatives reported in literature, which do not have a conjugated thiocarbonyl group in their structure. It has also been shown that the capacity as a reductive agent of DTF strongly depends on the substitution pattern of the base structure.

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“…The fitting procedure shows that a better agreement between theoretical and experimental data. After the protein is added to the solution, the recorded EIS spectrum are fitted to an electrical equivalent circuit comprised of two time constants in parallel, as shown in Figure d (inset), which is characteristic of an insulating layer over a conducting surface and yield the best fit among several other circuits. The Equivalent circuit (Inset in Figure d) is consisting of a electrolyte resistance ( R s ), an adsorbed protein resistance ( R f ), an adsorbed protein capacitance as a constant phase element (CPE 1 ), a double‐layer capacitance as a constant phase element (CPE 2 ), a charge transfer resistance ( R ct ) and a Warburg impedance ( Z w ).…”

Section: Resultsmentioning

confidence: 99%

Comparative Electrochemical Study of N‐, C‐terminal and Integral Centrin on Adsorption and Metal‐Binding Properties

et al. 2017

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The adsorption behavior of apo‐ciliate Euplotes octocarinatus centrin (EoCen) and N(C)‐terminal domain of EoCen (N(C)‐EoCen) at a glassy carbon (GC) electrode is studied by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Interestingly, the adsorption isotherms of C‐EoCen, N‐EoCen and EoCen at a GC surface differ from one another. It is considered to be associated with the different aggregation properties of three proteins. Furthermore, we analyze the metal‐binding properties of centrin and the followed changes in protein structure upon metal‐binding. Corresponding to the four binding sites of EoCen, it shows four no‐equiv signals of CV or EIS change. It indicates that the four different binding sites of EoCen can be discriminated by the EIS titration curves, which is in contrast with conventional use of spectral method. According to the electrostatic potential at the molecular surface of proteins, the favored orientation of N‐EoCen and C‐EoCen on the GC surface is modeled. These models can well explain the results of titration curves of Eu3+ to N(C)‐EoCen. This work has established the electrochemical methodology which can be used to measure metal‐binding sites involved and reveal the contribution of each metal site to the whole protein conformational change.

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Nanoarray‐Surfaces by Reconstitution of the Porin MspA into Stabilized Long‐Chain‐Lipid‐Monolayers at a Gold‐Surface

Cited by 8 publications

References 54 publications

Characterization of Nanostructured Surfaces Generated by Reconstitution of the Porin MspA from Mycobacterium smegmatis

Characterization of Nanostructured Surfaces Generated by Reconstitution of the Porin MspA from Mycobacterium smegmatis

Remarkable effect of the dithiafulvene structures on their capacity as reducing agents: Influence of conjugated thiocarbonyl group

Comparative Electrochemical Study of N‐, C‐terminal and Integral Centrin on Adsorption and Metal‐Binding Properties

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