Nanoassisted Laser Desorption-Ionization-MS Imaging of Tumors

Tata, Alessandra; Fernandes, Anna Maria A. P.; Santos, Vanessa G.; Alberici, Rosana M.; Araldi, Dionéia; Parada, Carlos Amílcar; Braguini, Welligton Luciano; Veronez, Luciana Chain; Bisson, Gabriela Silva; Reis, Fernando M. C. V.; Alberici, Luciane C.; Eberlin, Marcos N.

doi:10.1021/ac301202q

Cited by 39 publications

(32 citation statements)

References 40 publications

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“…Eberlin and coworkers reported application of NALDI‐MS to image tumors. The technique could successfully map several phospholipid biomarkers in a murine model of melanoma tumor …”

Section: Use Of Inorganic Materials As New Matricesmentioning

confidence: 99%

“…The technique could successfully map several phospholipid biomarkers in a murine model of melanoma tumor. 30 3 | USE OF SELF-ASSEMBLED SURFACES The LDI-MS of self-assembled monolayers was reported in a US patent by Han et al 31 The method involves a simple pretreatment of analyte samples in the form of mono layers formed on a surface with cationic solution, and analysis of resulting positively charged self-assembled mono layers by laser desorption/ionization. 31 No matrix was needed for the desorption of the monolayers.…”

Section: Graphenementioning

confidence: 99%

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Laser desorption ionization mass spectrometry: Recent progress in matrix‐free and label‐assisted techniques

Mandal

Singha

Addy

et al. 2017

Mass Spectrometry Reviews

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The MALDI-based mass spectrometry, over the last three decades, has become an important analytical tool. It is a gentle ionization technique, usually applicable to detect and characterize analytes with high molecular weights like proteins and other macromolecules. The earlier difficulty of detection of analytes with low molecular weights like small organic molecules and metal ion complexes with this technique arose due to the cluster of peaks in the low molecular weight region generated from the matrix. To detect such molecules and metal ion complexes, a four-prong strategy has been developed. These include use of alternate matrix materials, employment of new surface materials that require no matrix, use of metabolites that directly absorb the laser light, and the laser-absorbing label-assisted LDI-MS (popularly known as LALDI-MS). This review will highlight the developments with all these strategies with a special emphasis on LALDI-MS.

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“…Eberlin and coworkers reported application of NALDI‐MS to image tumors. The technique could successfully map several phospholipid biomarkers in a murine model of melanoma tumor …”

Section: Use Of Inorganic Materials As New Matricesmentioning

confidence: 99%

Section: Graphenementioning

confidence: 99%

Laser desorption ionization mass spectrometry: Recent progress in matrix‐free and label‐assisted techniques

Mandal

Singha

Addy

et al. 2017

Mass Spectrometry Reviews

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“…Fifty-six SN and fifty-eight SMM specimens were used to create and validate an algorithm classification model that could discriminate SN from SMM with 97% sensitivity and 90% specificity. Melanoma was also studied using nano-assisted laser desorption ionization (NALDI) MSI [149]. In this study, the authors searched for the changes in the lipidomic content of a murine model of melanoma treated by synthetic supplement containing phosphoethanolamine (PHO-S).…”

Section: Melanomamentioning

confidence: 99%

MALDI mass spectrometry imaging: A cutting‐edge tool for fundamental and clinical histopathology

Longuespée

Casadonte²,

Kriegsmann

et al. 2016

Proteomics Clinical Apps

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Histopathological diagnoses have been done in the last century based on hematoxylin and eosin staining. These methods were complemented by histochemistry, electron microscopy, immunohistochemistry (IHC), and molecular techniques. Mass spectrometry (MS) methods allow the thorough examination of various biocompounds in extracts and tissue sections. Today, mass spectrometry imaging (MSI), and especially matrix-assisted laser desorption ionization (MALDI) imaging links classical histology and molecular analyses. Direct mapping is a major advantage of the combination of molecular profiling and imaging. MSI can be considered as a cutting edge approach for molecular detection of proteins, peptides, carbohydrates, lipids, and small molecules in tissues. This review covers the detection of various biomolecules in histopathological sections by MSI. Proteomic methods will be introduced into clinical histopathology within the next few years.

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“…As an example, NALDI-MS images of tumors through lipid analysis was demonstrated to be an encouraging technology for biomarker discovery [79].…”

Section: Imaging Msmentioning

confidence: 99%

Direct Mass Spectrometry-Based Approaches in Metabolomics

Ibáñez

García‐Cañas

Valdés

et al. 2014

Fundamentals of Advanced Omics Technologies: From Genes to Metabolites

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Metabolomics is one of the newest omics technologies concerned with the identification and quantification of small molecules in a high-throughput manner. Considering the number of different types of metabolites present in a wide dynamic range of concentrations in any single living system, still actual analytical technologies can only capture a part of the metabolome.Currently MS-based approaches yield a higher sensitivity than NMR when analyzing minimal amounts of complex mixtures. Most MS-based approaches in metabolomics involve a physical/chemical purification/fractionation prior to MS analysis, to avoid sample matrix effects, at the expenses of low high-throughput performance. In the quest to achieve the maximum highthroughput production of metabolite information in the largest possible number of samples an extensive array of direct ionization or desorption/ionization techniques have been developed and combined. In the present Chapter an overview of the main desorption/ionization techniques coupled to MS applied to direct metabolite profiling or fingerprinting is presented.

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Nanoassisted Laser Desorption-Ionization-MS Imaging of Tumors

Cited by 39 publications

References 40 publications

Laser desorption ionization mass spectrometry: Recent progress in matrix‐free and label‐assisted techniques

Laser desorption ionization mass spectrometry: Recent progress in matrix‐free and label‐assisted techniques

MALDI mass spectrometry imaging: A cutting‐edge tool for fundamental and clinical histopathology

Direct Mass Spectrometry-Based Approaches in Metabolomics

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