Nanobiolistics: An Emerging Genetic Transformation Approach

Cunningham, F. J.; Demirer, Gözde S.; Goh, Natalie S.; Zhang, Huan; Landry, Markita P.

doi:10.1007/978-1-0716-0356-7_7

Cited by 9 publications

(5 citation statements)

References 96 publications

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“…The biolistic method delivers molecules into plant nuclei using accelerated nano-sized particles coated with nucleic acids or RNP complexes ( Figure 2 a) [ 76 ]. However, there are several disadvantages to this approach, including the random integration of the cargo DNA at multiple sites in the genome and the high cost of the equipment and reagents.…”

Section: Methods For Obtaining Gene-edited Plantsmentioning

confidence: 99%

Recent Advances in Tomato Gene Editing

Larriba,

Yaroshko,

Pérez-Pérez

2024

IJMS

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The use of gene-editing tools, such as zinc finger nucleases, TALEN, and CRISPR/Cas, allows for the modification of physiological, morphological, and other characteristics in a wide range of crops to mitigate the negative effects of stress caused by anthropogenic climate change or biotic stresses. Importantly, these tools have the potential to improve crop resilience and increase yields in response to challenging environmental conditions. This review provides an overview of gene-editing techniques used in plants, focusing on the cultivated tomatoes. Several dozen genes that have been successfully edited with the CRISPR/Cas system were selected for inclusion to illustrate the possibilities of this technology in improving fruit yield and quality, tolerance to pathogens, or responses to drought and soil salinity, among other factors. Examples are also given of how the domestication of wild species can be accelerated using CRISPR/Cas to generate new crops that are better adapted to the new climatic situation or suited to use in indoor agriculture.

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Section: Methods For Obtaining Gene-edited Plantsmentioning

confidence: 99%

Recent Advances in Tomato Gene Editing

Larriba,

Yaroshko,

Pérez-Pérez

2024

IJMS

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“…The absorption of passively absorbable NPs at the shooting surface is made possible by the micro-or nanoscale exclusion sizes of the natural plant apertures, such as the hydathodes, stomata, bark texture, and stigma. More knowledge of plant physiology and anatomy is required to completely understand the dynamics of plant-NP interactions [102]. On the surface of a plant's shoots, a cuticle consisting of biopolymers and associated waxes creates a lipophilic bar- Although ENMs may be applied to the roots or any other vegetative portion of the plant, they are most often seen in the leaves when given to plants.…”

Section: Plant-nanomaterials Interactionsmentioning

confidence: 99%

Nano-Biofertilizer Formulations for Agriculture: A Systematic Review on Recent Advances and Prospective Applications

Garg,

Sridhar,

Stephen Inbaraj

et al. 2023

Bioengineering

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In the twenty-first century, nanotechnology has emerged as a potentially game-changing innovation. Essential minerals are mostly unavailable in modern cropping systems without the application of synthetic fertilizers, which have a serious negative impact on the ecosystem. This review focuses on the coupling of nanoparticles with biofertilizers to function as nano-biofertilizers (NBFs), which may ensure world food security in the face of the rising population. The inoculation of plants with NBFs improves plant development and resistance to stress. Metallic nanoparticles as well as organic components comprising polysaccharide and chitosan may be encapsulated, utilizing microbe-based green synthesis to make NBFs, which circumvents the limitations of conventional chemical fertilizers. The application of NBFs is just getting started, and shows more promise than other approaches for changing conventional farming into high-tech “smart” farming. This study used bibliographic analysis using Web of Science to find relevant papers on “nano biofertilizers”, “plants”, and “agriculture”. These subjects have received a lot of attention in the literature, as shown by the co-citation patterns of these publications. The novel use of nanotechnology in agriculture is explored in this research work, which makes use of the unique characteristics of nanoscale materials to address urgent concerns including nutrient delivery, crop protection, and sustainable farming methods. This study attempts to fill in some of the gaps in our knowledge by discussing the formulation, fabrication, and characterization of NBFs, as well as elucidating the mechanisms by which NBFs interact with plants and how this benefits the ability of the plant to withstand biotic and abiotic stress brought about by climate change. This review also addresses recent developments and future directions in farming using NBF formulations in the field.

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“…Several alternative approaches for reagent delivery which were initially developed in animal cells, are being explored in plants ( Ghogare et al., 2021 ). For example, a biolistics approach using nanoparticles offers a less harmful delivery method compared to larger microparticles, which may reduce delivery damage, a common issue encountered in plants due to the presence of a cell wall ( Zhang et al., 2019 ; Cunningham et al., 2020 ). Most excitingly, delivery mediated by viral vectors can completely bypass the need for regeneration which is an extremely promising prospect for editing hard to regenerate CWRs, however this method is limited by its delivery capacity ( Shan-e-Ali Zaidi and Mansoor, 2017 ).…”

Section: Modern Breeding and Cwrsmentioning

confidence: 99%

Application of crop wild relatives in modern breeding: An overview of resources, experimental and computational methodologies

et al. 2022

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Global agricultural industries are under pressure to meet the future food demand; however, the existing crop genetic diversity might not be sufficient to meet this expectation. Advances in genome sequencing technologies and availability of reference genomes for over 300 plant species reveals the hidden genetic diversity in crop wild relatives (CWRs), which could have significant impacts in crop improvement. There are many ex-situ and in-situ resources around the world holding rare and valuable wild species, of which many carry agronomically important traits and it is crucial for users to be aware of their availability. Here we aim to explore the available ex-/in- situ resources such as genebanks, botanical gardens, national parks, conservation hotspots and inventories holding CWR accessions. In addition we highlight the advances in availability and use of CWR genomic resources, such as their contribution in pangenome construction and introducing novel genes into crops. We also discuss the potential and challenges of modern breeding experimental approaches (e.g. de novo domestication, genome editing and speed breeding) used in CWRs and the use of computational (e.g. machine learning) approaches that could speed up utilization of CWR species in breeding programs towards crop adaptability and yield improvement.

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Nanobiolistics: An Emerging Genetic Transformation Approach

Cited by 9 publications

References 96 publications

Recent Advances in Tomato Gene Editing

Recent Advances in Tomato Gene Editing

Nano-Biofertilizer Formulations for Agriculture: A Systematic Review on Recent Advances and Prospective Applications

Application of crop wild relatives in modern breeding: An overview of resources, experimental and computational methodologies

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