Nanobubble-Enhanced Antimicrobial Agents: A Promising Approach for Regenerative Endodontics

Shawli, Hassan; Iohara, Koichiro; Tarrosh, Mohamed; Huang, George T.‐J.; Nakashima, Mitsuyoshi; Azim, Adham A.

doi:10.1016/j.joen.2020.06.002

Cited by 20 publications

(16 citation statements)

References 33 publications

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“…Residual microorganisms in root canal systems may lead to unsuccessful treatment; thus, safer and more efficient disinfection strategies, such as nanobubble-enhanced antimicrobial agents, are needed in patients with severe periapical lesions rather than routine disinfection [149].…”

Section: Challenge #2: Specific Therapeutic Procedures With Different Indicationsmentioning

confidence: 99%

Functional Dental Pulp Regeneration: Basic Research and Clinical Translation

Xie

Shen

Zhan

et al. 2021

IJMS

107

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Pulpal and periapical diseases account for a large proportion of dental visits, the current treatments for which are root canal therapy (RCT) and pulp revascularisation. Despite the clinical signs of full recovery and histological reconstruction, true regeneration of pulp tissues is still far from being achieved. The goal of regenerative endodontics is to promote normal pulp function recovery in inflamed or necrotic teeth that would result in true regeneration of the pulpodentinal complex. Recently, rapid progress has been made related to tissue engineering-mediated pulp regeneration, which combines stem cells, biomaterials, and growth factors. Since the successful isolation and characterisation of dental pulp stem cells (DPSCs) and other applicable dental mesenchymal stem cells, basic research and preclinical exploration of stem cell-mediated functional pulp regeneration via cell transplantation and cell homing have received considerably more attention. Some of this effort has translated into clinical therapeutic applications, bringing a ground-breaking revolution and a new perspective to the endodontic field. In this article, we retrospectively examined the current treatment status and clinical goals of pulpal and periapical diseases and scrutinized biological studies of functional pulp regeneration with a focus on DPSCs, biomaterials, and growth factors. Then, we reviewed preclinical experiments based on various animal models and research strategies. Finally, we summarised the current challenges encountered in preclinical or clinical regenerative applications and suggested promising solutions to address these challenges to guide tissue engineering-mediated clinical translation in the future.

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Section: Challenge #2: Specific Therapeutic Procedures With Different Indicationsmentioning

confidence: 99%

Functional Dental Pulp Regeneration: Basic Research and Clinical Translation

Xie

Shen

Zhan

et al. 2021

IJMS

107

View full text Add to dashboard Cite

show abstract

“…SEM and CLSM are frequently used to evaluate dentinal tubule penetration of root canal irrigation solutions, intracanal medicaments and root canal sealers in endodontics [20]. In order to obtain an image in SEM, dentin discs must be dried in the oven and exposed to alcohol and high vacuum applications.…”

Section: Discussionmentioning

confidence: 99%

Effect of different final irrigation agitation techniques on root canal dentin tubule penetration of nanoparticle calcium hydroxide dressing

2023

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This study is aimed to assess the efficacy of different final irrigation agitation techniques on dentin tubule penetration of nanoparticle calcium hydroxide (NCH) dressing. Ninety‐six extracted upper incisors were shaped up to #40file. Then, Four experimental groups were formed according to the final irrigation procedure: conventional needle irrigation (CNI), manual dynamic agitation (MDA), sonic agitation (SA) and ultrasonic irrigant agitation (UIA). According to the intracanal drug used, these groups were divided into two subgroups: calcium hydroxide (CH) and NCH. Prepared CH preparations were labelled with Rhodamine B, and CH or NCH were placed in root canals. In the UIA group, both CH and NCH had the highest penetration depth and percentage compared to the other groups (p < 0.05). The penetration depth and percentage of NCH in the UIA and SA groups were significantly higher than in the CH groups (p < 0.05). UIA is more effective than other groups in increasing dentinal tubule penetration of CH and NCH.

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“…After the first screening (Step 1), 55 articles were selected and submitted to a full-text review (Step 2). Then, 19 studies were excluded, in which 13 (Arslan et al, 2014;Bosaid et al, 2020;Buldur et al, 2019;Ivica et al, 2020;Kandemir Demirci et al, 2020;Nagata et al, 2014;Nerness et al, 2016;Saghiri et al, 2016;Shawli et al, 2020;Ustun et al, 2018;Widbiller et al, 2018;Yassen et al, 2014Yassen et al, , 2015 were outside of the proposed theme, and 6 had no control group (Ferreira et al, 2020;Galler et al, 2011;Graham et al, 2006;Hristov et al, 2018;Tomson et al, 2007;Trevino et al, 2011; Figure 1). A total of 36 studies were included in the qualitative analysis and are presented in Tables 1, 2 and 3. The assessed Cohen's kappa coefficient for interinvestigator agreement during the studies' selection was 0.843 for PubMed, 0.970 for Scopus, 1.000 for the Cochrane Library and OpenGrey, 0.925 for the Web of Science and 0.876 for the Embase.…”

Section: Study Selectionmentioning

confidence: 99%

Influence of ethylenediaminetetraacetic acid on regenerative endodontics: A systematic review

et al. 2022

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Background The effects of ethylenediaminetetraacetic acid (EDTA) on regenerative endodontic procedures (REPs) are controversial, because, despite releasing growth factors from dentine, some studies show negative effects on cell behaviour. Objectives The aim of the study was to investigate the influence of the use of EDTA in REP on the growth factors’ release, cell behaviour and tissue regeneration. Methods A systematic search was conducted (PubMed/Medline, Scopus, Cochrane Library, Web of Science, Embase, OpenGrey and reference lists) up to February 2021. Only in vivo and in vitro studies evaluating the effects of EDTA on the biological factors of dentine, pulp/periapical tissues and cell behaviour were eligible. Studies without a control group or available full text were excluded. The growth factors’ release was the primary outcome. Risk of bias in the in vitro and in vivo studies was performed according to Joanna Briggs Institute's Checklist and SYRCLE’s RoB tool, respectively. Results Of the 1848 articles retrieved, 36 were selected. Amongst these, 32 were in vitro, three animal studies and one with both models. The EDTA concentrations ranged from 3% to 15%, at different times. Regarding growth factors’ release (17 studies), 15 studies found significant transforming growth factor (TGF)‐β release after dentine conditioning with EDTA, and most found no influence on vascular endothelial growth factor release. Regarding cell behaviour (26 studies), eight studies showed no influence of EDTA‐treated dentine on cell viability, whereas, five, nine and six studies showed higher cell migration, adhesion and differentiation respectively. No influence of EDTA conditioning was observed in animal studies. In vitro studies had a low risk of bias, whereas animal studies had high risk of bias. Meta‐analysis was unfeasible. Discussion This review found that EDTA increased TGF‐β release and improved cell activity. However, well‐designed histological analyses using immature teeth models are needed. Conclusions High‐quality in vitro evidence suggests that EDTA‐treated dentine positively influences TGF‐β release, cell migration, attachment and differentiation; further research to evaluate its influence on tissue regeneration is necessary due to low methodological quality of the animal studies.

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Nanobubble-Enhanced Antimicrobial Agents: A Promising Approach for Regenerative Endodontics

Cited by 20 publications

References 33 publications

Functional Dental Pulp Regeneration: Basic Research and Clinical Translation

Functional Dental Pulp Regeneration: Basic Research and Clinical Translation

Effect of different final irrigation agitation techniques on root canal dentin tubule penetration of nanoparticle calcium hydroxide dressing

Influence of ethylenediaminetetraacetic acid on regenerative endodontics: A systematic review

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