Nanocapsules Loaded with Iron-Saturated Bovine Lactoferrin have Antimicrobial Therapeutic Potential and Maintain Calcium, Zinc and Iron Metabolism

Abstract

The study concludes that bLf and nanoformulated Fe-bLf are more effective in the treatment of Salmonella-infected mice than ciprofloxacin.

Lactoferrin: Balancing Ups and Downs of Inflammation Due to Microbial Infections

“…The latter was evidenced by up- and down-modulation of cytokines involved in innate and adaptive immune responses as well on hematopoietic cytokines, with a key role in the generation of both granulocyte (PMN neutrophils) or agranulocyte (monocytes/macrophages) phagocytes [6]. Thus, iron-loaded bLf nanocapsules seem to evoke the convergence of innate and adaptive immune responses of pro- and anti-inflammatory cytokines resulting in the protection toward typhoid infection and concomitant intestinal inflammation.…”

“…The biotechnological development of formulations of Lf as nanoparticles for potential clinical use, in addition to Lf-hydrolysate, and native Lf alone or in combination with probiotics, may have application for the control of infections and inflammation [6,74,130,184,190,191]. …”

“…This review is focused on the modulatory impact of Lf on the inflammatory response induced by infectious microorganisms, mainly in the gastrointestinal and respiratory tracts. The study of modulatory properties of Lf on inflammatory response has impacted in the biotechnological development of nanoparticle Lf formulations of potential clinical implications [6]. …”

LNA aptamer based multi-modal, Fe 3 O 4 -saturated lactoferrin (Fe 3 O 4 -bLf) nanocarriers for triple positive (EpCAM, CD133, CD44) colon tumor targeting and NIR, MRI and CT imaging

“…Different microRNAs (miRNAs) responsible for iron regulation in different organs were identified and it was found that the NCs inhibited survivin and its different isoforms to induce apoptosis via both extrinsic as well as intrinsic pathways [10]. We have delivered drug (Taxol) and FebLf to treat colon [9,10] and breast xenograft cancers [11] and Salmonella typhimurium infected mice [12] using these NCs, without inducing any cytotoxicity. More recently, we validated the efficacy and safety of these nanocapsules to deliver anti-inflammatory Fe-bLf [13], and Indian herbal bioactive from Lakshadi Guggul glycoprotein (LG-GP) [14] to inflamed joints in collagen-induced DBA/1 arthritis (CIA) model.…”

“…We have elaborately validated and deciphered the internalization and the anti-cancer activity of these NCs in both in vitro and in vivo models of breast/colon cancers [19,21], microbial infections [12] and osteoarthritis [13]. We also compared the number of RBCs in the treatment groups and in control mice and found that nanolactoferrin treated mice were healthy with improved blood cell counts [9,10,11].…”