Nanocellulose-collagen-apatite composite associated with osteogenic growth peptide for bone regeneration

Abstract

Despite advances in the field of biomaterials for bone repair/regeneration, some challenges for developing an ideal bone substitute need to be overcome. Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes. The BC-COL nanocomposites were successfully obtained by carbodiimide-mediated coupling as demonstrated by spectroscopy analysis. SEM, FTIR and P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite. The OGP-containing (BC-COL)-Ap stimulated the early development of the osteoblastic phenotype. Additionally, the association among collagen, apatite, and OGP peptides enhanced cell growth compared with OGP-containing BC-Ap. Furthermore, none of the nanocomposites showed cytotoxic, genotoxic or mutagenic effects. These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications.

Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration

“…Similar results were obtained in other studies stating that the incorporation of OGP to bacterial cellulose enhanced the cell growth and proliferation. 63 Another study has also reported that OGP immobilized on the gradient substrate synthesized via click chemistry can enhance the MC3T3-E1 osteoblast proliferation. 64 Moreover, ALP activity analysis and immunouorescence staining suggested that OGP could signicantly promote the osteogenic differentiation of the hMSCs.…”

Nanocellulose, a Versatile Green Platform: From Biosources to Materials and Their Applications

“…Bacterial cellulose has emerged as an effective biopolymer and extensively used as a matrix for the synthesis of biomaterials for tissue engineering and regenerative medicine applications due to its high mechanical properties, bio and cyto compatibilities, and low cytotoxicity 62,67,375 . Recentely, Saska and co-workers showed that a nanocellulose-collagen-apatite composite associated with osteogenic growth peptide can be effectively used for bone regeneration 376 .…”

“…The products obtained by Granja, et al were crystalline monoesters, which swelled in water, and which could be sterilized with gamma irradiation without being significantly damaged. In another study, a hybrid composite for bone regeneration was constructed through the covalent attachment of collagen to a bacterial cellulose matrix376 . Carbonate apatite and osteogenic growth peptide were incorporated into this composite in order to form a bone-like material capable of encouraging supported cell growth.Wounds present a risk of infection and thus promoting healing can avoid more serious health problems.…”

Cellulose Nanomaterials—Binding Properties and Applications: A Review

“…There are important properties for a better bone regeneration process but facilitating phosphate and calcium deposition are among the most important ones. Saska et al [ 178 ], used BC and collagen to produce a nanocomposite with good binding properties as a suitable option for attaching the collagen to apatite for bone restoration. Results showed that BC-COL-apatite nanocomposite helped the regeneration process with no evidence of cytotoxic, genotoxic, or mutagenic effects.…”