Nanocellulose films with multiple functional nanoparticles in confined spatial distribution

Abstract

Laminated multifunctional bacterial cellulose nanocomposite with a millefeuille topographic distribution of inorganic nanoparticles.

Bacterial nanocellulose as a corneal bandage material: a comparison with amniotic membrane

“…Bacterial nanocellulose films were obtained following our established protocol detailed elsewhere. 57 Briefly, a commercial Komagataeibacter xylinus (K. xylinus) strain (NCIMB 5346 from CECT, Valencia, Spain) was inoculated on 6 mL of Hestrin-Schramm (HS) medium (1.15 g citric acid, 6.8 g Na 2 HPO 4 •12H 2 O, 5 g peptone, 5 g yeast, and 20 g dextrose for 1 L of Milli-Q water) and incubated statically for 7 days at 30°C. This bacterial culture was further diluted 1 : 15 with fresh HS medium and cultured on 24-well plates (2 mL per well) during 3 days.…”

Self‐Assembly of Mechanoplasmonic Bacterial Cellulose–Metal Nanoparticle Composites

“…[2,20] The porous structure of the fibrillar BC network enables diffusion of metal ions and reducing agents into the material where the ether oxygen and hydroxyl groups of cellulose can sequester the ions and stabilize the resulting metal NPs. [21] Although the cellulose can also contribute to the reduction of the metal ions, [2,22] reducing agents such as borhydride, hydrazine, hydroxylamine, ascorbic acid, [23] and triethanolamine [24] are widely used, as well as microwave [25] and UVradiation. [26] In order to prevent NP aggregation after synthesis, various polymers can be added to stabilize the NPs.…”

In vivosoft tissue reinforcement with bacterial nanocellulose

“…BNC films were obtained as previously described in Roig-Sanchez S. et al 27 In brief, Komagataeibacter xylinus (K.…”

“…To obtain the dry and flat BNC films used in the mechanical and in vivo tests, BNC hydrogels were placed between two Teflon papers at 60°C and with a 2 kg weight on top for 12 h as previously described. 27 Systems with two and three BNC layers were prepared by drying size-matched BNC pellicles in close contact following the same procedure. To achieve a smooth interface between the BNC films, the superficial water was removed by blotting BNC with filter paper and air bubble formation was avoided by applying manual pressure.…”

“…The multilayers do not suffer delamination even when immersed in a liquid solution. 27 Accordingly, laminated patches were created by assembling 2 or 3 wet BNC films and subjected to the same mechanical characterization as the single BNC layers. Fig.…”