Nanocomposite membrane integrated phage enrichment process for the enhancement of high rate phage infection and productivity

Anam, Giridhar Babu; Yadav, Seema; Ayyaru, Sivasankaran; Ahn, Young‐Ho

doi:10.1016/j.bej.2020.107740

Cited by 5 publications

(3 citation statements)

References 35 publications

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Section: Enhancing Bactericidal Efficiencymentioning

confidence: 99%

“…108 A lactonase enzyme encoding gene was inserted into T7 phage genome, which endows the engineered phage capability of inhibiting biofilm formation of both P. aeruginosa and E. coli since this enzyme could quench QS system. 50 In addition, synthetic M13 phagemid could be used as a delivery vector to upload genes for antimicrobial peptides (AMPs), or toxin proteins. 109 Recombinant phagemid DNA is first transformed into a production strain harboring a helper plasmid to amplify abundant functional phagemids.…”

Section: Enhancing Bactericidal Efficiencymentioning

confidence: 99%

“…49 Phages can be modified by chemical modification to improve the efficiency of sterilization or bacteria detection. 50,51 Phage can be visualized through labeling its genome with fluorescence dye, such as YOYO, Cl-YO, Cl-YO-Et, Cl-YO-Bu, and the labeled phages are observed by flow cytometry and microscope. 52,53 Since fluorescence dye may affect the ejection of the phage genome, dye selection is important.…”

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confidence: 99%

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Advance on Engineering of Bacteriophages by Synthetic Biology

Sun¹,

Shen²,

Zhang³

et al. 2023

IDR

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Since bacteriophages (phages) were firstly reported at the beginning of the 20th century, the study on them experiences booming-fading-emerging with discovery and overuse of antibiotics. Although they are the hotspots for therapy of antibiotic-resistant strains nowadays, natural phage applications encounter some challenges such as limited host range and bacterial resistance to phages. Synthetic biology, one of the most dramatic directions in the recent 20-years study of microbiology, has generated numerous methods and tools and has contributed a lot to understanding phage evolution, engineering modification, and controlling phage-bacteria interactions. In order to better modify and apply phages by using synthetic biology techniques in the future, in this review, we comprehensively introduce various strategies on engineering or modification of phage genome and rebooting of recombinant phages, summarize the recent researches and potential directions of phage synthetic biology, and outline the current application of engineered phages in practice.

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Section: Enhancing Bactericidal Efficiencymentioning

confidence: 99%