Nanoemulsified <i>Eucalyptus globulus</i> Essential Oil Against Mosquito <i>Aedes aegypti</i> (L.)

Abstract

In the present study, nanoemulsified essential oil of Eucalyptus globulus was analysed for its chemical composition and larvicidal activity against mosquito Aedes aegypti. GC-MS analysis revealed presence of total 22 compounds, of which the major ones were 1, 8-cineole (42.16%) and P-cymene (14.81%). Non polar fractions of oil were sonicated by mixing it in water with Tween 20 in different ratios, and 1:2 ratio was found to be the most stable. This was characterized using TEM and found to have spherical droplets of 20-40 nm. Nanoemulsified oil was found to be effective at 70 ppm with LC50 and LC90 of 51.95 and 64.21 ppm, respectively. SEM studies showed significant shrinkage and disruption of various larval body parts post treatment.