Nanoemulsion Formulation of <i>Ocimum gratissimum</i> Essential Oil and Its Antifungal Activity Against <i>Penicillium digitatum</i>

Mahajan, Rishabh; Tandon, Ritu; Kalia, Anu; Mahajan, B. V. C.

doi:10.1166/jnn.2021.19008

Cited by 15 publications

(5 citation statements)

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“…Insignificant surfactant diffusion and adsorption to the surface of newly formed droplets could have caused an insignificant reduction in MDD (Kentish et al, 2008). Among all ratios, 1:1 OSR was observed to be the most optimum and these findings were in agreement with those of Mahajan et al (2021) and Barzegar et al (2018).…”

Section: Mean Droplet Diameter (Mdd) and Pdi Of Gpeo-nesupporting

confidence: 75%

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Grapefruit peel essential oil nanoemulsions: preparation, characterisation, stability and antifungal potential against Penicillium digitatum of Kinnow

Dudeja

Tandon

Kalia

2023

Int J of Food Sci Tech

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Summary Hydrophobicity of essential oils poses a big hindrance for harnessing their diverse antimicrobial portfolio. The study was therefore planned to develop and explore grapefruit peel essential oil nanoemulsions (GPEO‐NEs) as an eco‐friendly hydrophilic alternate against Penicillium digitatum, a prominent postharvest fungus of kinnow. GPEO‐NEs were prepared by mixing GPEO and surfactant (Tween 80) in different ratios (v/v) viz 1:0.5–1:3 by bath and probe sonication. The 1:1 OSR GPEO‐NE (20 min) by probe sonication exhibited consistent droplet diameter (96.6 nm) and PDI (0.247). All GPEO‐NEs were evaluated for antifungal potential against P. digitatum using poisoned food technique. The GPEO‐NEs (1:1 OSR; 20 min) revealed the best efficacy (88.6%) as compared to pure GPEO (76.6%). Optical microscopy further revealed stronger suppressive activity of GPEO‐NEs for spore germination and hyphal distortion of P. digitatum than pure oil. The GPEO‐NEs can thus serve as a green alternative to synthetic fungicides for managing postharvest losses in kinnow by using these as coatings.

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Section: Mean Droplet Diameter (Mdd) and Pdi Of Gpeo-nesupporting

confidence: 75%

“…Among all ratios, 1:1 OSR was observed to be the most optimum and these findings were in agreement with those of Mahajan et al . (2021) and Barzegar et al . (2018).…”

Section: Resultsmentioning

confidence: 95%

Grapefruit peel essential oil nanoemulsions: preparation, characterisation, stability and antifungal potential against Penicillium digitatum of Kinnow

Dudeja

Tandon

Kalia

2023

Int J of Food Sci Tech

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“…have been used to improve the antimicrobial activity of the essential oil of Ocimum gratissimum and has been demonstrated in studies by Mahajan et al, (2021); Araujo-Silva et al, (2016); Ontao et al, (2021);and Okonkwo et al, (2020).…”

Section: Nanoemulsions Of Ocimum Gratissimummentioning

confidence: 99%

Ocimum Gratissimum essential oil: A review of extraction methods, phytochemical constituents, pharmacological uses and formulation approaches

Ikeotuonye,

Uronnachi,

Nwakile

et al. 2023

jcbr

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Ocimum gratissimum is a plant native to Africa. Its leaves have been used in local dishes over the years, with documented evidence of varying pharmacological uses of its essential oil. This review focuses on the extraction methods, phytochemical constituents, pharmacological uses and formulation approaches of Ocimum gratissimum essential oil. The traditional extraction methods that have been widely used include the solvent, cold press and hydrodistillation methods. However, newer methods such as steam distillation, ultrasound and microwave assisted hydrodistillation, supercritical fluid extraction, pressurized liquid extraction and pressurized hot water extraction methods have not been fully reported. These methods provide more yield of the essential oil compared with the traditional methods and their processes are less laborious. Furthermore, they are often limited to analytical applications as they cannot handle large quantities of samples. The phytochemical constituents of Ocimum gratissimum essential oil vary, based on location of the plant and the time of the season the plant parts were harvested for extraction. The African species have more thymol than eugenol. Other notable constituents are saponins, terpenes and flavonoids in both African species and others. The essential oil of Ocimum gratissimum bears the scent of the plant and other important properties such as protection of the plant from pests and to attract insects for cross-pollination. The essential oil of this plant has been noted to possess various pharmacological properties which explains its use in inflammatory conditions and the treatment of diarrhoeal disease, wound healing and cerebrovascular disorder, amongst others. The essential oil of Ocimum gratissimum has been embedded in gels, cream, silver nanoparticles and nanoemulsion to improve its pharmacological activity and bioavailability. This review article highlights the potentials of the essential oil of this ubiquitous plant as a therapeutic source for the treatment of different tropical diseases.

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“…Penicillium Species Mahajan et al (2021) tested Ocimum gratissimum EO and its nanoemulsions against P. digitatum of kinnow mandarin fruit by the poisoned food technique. The nanoemulsion exhibited stronger growth inhibition (1 × 10 4 CFU ml −1 , 96%) than unencapsulated oil (13 × 10 4 CFU ml −1 , 85%) on the 15 th day of incubation.…”

Section: Efficacy Of Essential Oil Nanoemulsion Againstmentioning

confidence: 99%

Essential Oil Nanoemulsion as Eco-Friendly and Safe Preservative: Bioefficacy Against Microbial Food Deterioration and Toxin Secretion, Mode of Action, and Future Opportunities

et al. 2021

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Microbes are the biggest shareholder for the quantitative and qualitative deterioration of food commodities at different stages of production, transportation, and storage, along with the secretion of toxic secondary metabolites. Indiscriminate application of synthetic preservatives may develop resistance in microbial strains and associated complications in human health with broad-spectrum environmental non-sustainability. The application of essential oils (EOs) as a natural antimicrobial and their efficacy for the preservation of foods has been of present interest and growing consumer demand in the current generation. However, the loss in bioactivity of EOs from fluctuating environmental conditions is a major limitation during their practical application, which could be overcome by encapsulating them in a suitable biodegradable and biocompatible polymer matrix with enhancement to their efficacy and stability. Among different nanoencapsulated systems, nanoemulsions effectively contribute to the practical applications of EOs by expanding their dispersibility and foster their controlled delivery in food systems. In line with the above background, this review aims to present the practical application of nanoemulsions (a) by addressing their direct and indirect (EO nanoemulsion coating leading to active packaging) consistent support in a real food system, (b) biochemical actions related to antimicrobial mechanisms, (c) effectiveness of nanoemulsion as bio-nanosensor with large scale practical applicability, (d) critical evaluation of toxicity, safety, and regulatory issues, and (e) market demand of nanoemulsion in pharmaceuticals and nutraceuticals along with the current challenges and future opportunities.

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Nanoemulsion Formulation of Ocimum gratissimum Essential Oil and Its Antifungal Activity Against Penicillium digitatum

Cited by 15 publications

References 0 publications

Grapefruit peel essential oil nanoemulsions: preparation, characterisation, stability and antifungal potential against Penicillium digitatum of Kinnow

Grapefruit peel essential oil nanoemulsions: preparation, characterisation, stability and antifungal potential against Penicillium digitatum of Kinnow

Ocimum Gratissimum essential oil: A review of extraction methods, phytochemical constituents, pharmacological uses and formulation approaches

Essential Oil Nanoemulsion as Eco-Friendly and Safe Preservative: Bioefficacy Against Microbial Food Deterioration and Toxin Secretion, Mode of Action, and Future Opportunities

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