Nanoengineered implant as a new platform for regenerative nanomedicine using 3D well-organized human cell spheroids

Keller, Laetitia; Idoux-Gillet, Ysia; Wagner, Quentin; Eap, Sandy; Brasse, David; Schwinté, Pascale; Arruebo, Manuel; Benkirane‐Jessel, Nadia

doi:10.2147/ijn.s116749

Cited by 18 publications

(14 citation statements)

References 53 publications

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“…Spheroids present a tool for targeted and directed colonization of defined scaffold areas by the desired cell type. Hence, a spheroid-based seeding has become a well-established method in regenerative medicine, not only with stem cells [44], endothelial cells [45] or osteoblasts [46] but also with ligament-and particularly ACL-derived fibroblasts, as previously shown [15,38]. The results after 14 days indicate that long-term cultivation is possible with both the dynamical and statical (spheroid-based) seeding approaches.…”

Section: Discussionmentioning

confidence: 73%

Enhanced Growth of Lapine Anterior Cruciate Ligament-Derived Fibroblasts on Scaffolds Embroidered from Poly(l-lactide-co-ε-caprolactone) and Polylactic Acid Threads Functionalized by Fluorination and Hexamethylene Diisocyanate Cross-Linked Collagen Foams

Gögele

Hahn

Elschner

et al. 2020

IJMS

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Reconstruction of ruptured anterior cruciate ligaments (ACLs) is limited by the availability and donor site morbidity of autografts. Hence, a tissue engineered graft could present an alternative in the future. This study was undertaken to determine the performance of lapine (L) ACL-derived fibroblasts on embroidered poly(l-lactide-co-ε-caprolactone) (P(LA-CL)) and polylactic acid (PLA) scaffolds in regard to a tissue engineering approach for ACL reconstruction. Surface modifications of P(LA-CL)/PLA by gas-phase fluorination and cross-linking of a collagen foam using either ethylcarbodiimide (EDC) or hexamethylene diisocyanate (HMDI) were tested regarding their influence on cell adhesion, growth and gene expression. The experiments were performed using embroidered P(LA-CL)/PLA scaffolds that were seeded dynamically or statically with LACL-derived fibroblasts. Scaffold cytocompatibility, cell survival, numbers, metabolic activity, ultrastructure and sulfated glycosaminoglycan (sGAG) synthesis were evaluated. Quantitative real-time polymerase chain reaction (QPCR) revealed gene expression of collagen type I (COL1A1), decorin (DCN), tenascin C (TNC), Mohawk (MKX) and tenomodulin (TNMD). All tested scaffolds were highly cytocompatible. A significantly higher cellularity and larger scaffold surface areas colonized by cells were detected in HMDI cross-linked and fluorinated scaffolds compared to those cross-linked with EDC or without any functionalization. By contrast, sGAG synthesis was higher in controls. Despite the fact that the significance level was not reached, gene expressions of ligament extracellular matrix components and differentiation markers were generally higher in fluorinated scaffolds with cross-linked collagen foams. LACL-derived fibroblasts maintained their differentiated phenotype on fluorinated scaffolds supplemented with a HMDI cross-linked collagen foam, making them a promising tool for ACL tissue engineering.

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Section: Discussionmentioning

confidence: 73%

Enhanced Growth of Lapine Anterior Cruciate Ligament-Derived Fibroblasts on Scaffolds Embroidered from Poly(l-lactide-co-ε-caprolactone) and Polylactic Acid Threads Functionalized by Fluorination and Hexamethylene Diisocyanate Cross-Linked Collagen Foams

Gögele

Hahn

Elschner

et al. 2020

IJMS

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“…(Visscher et al, 2017). Another novel tissue engineering application for the early-stage treatment of bone lesions presents living bone implants that combine nanofibrous PCL scaffold for cell colonization and 3D bone cell spheroid formation (OB bone cells) is highly recommended before the need for bone substitutes (Keller et al, 2017).…”

Section: D Bioprintingmentioning

confidence: 99%

“…A new approach for breast reconstruction postmastectomy is finding a fresh paradigm with implantation of PCL scaffolds embedding the patient's adipose derived stem cells (Visscher et al, 2017). Another novel tissue engineering application for the early‐stage treatment of bone lesions presents living bone implants that combine nanofibrous PCL scaffold for cell colonization and 3D bone cell spheroid formation (OB bone cells) is highly recommended before the need for bone substitutes (Keller et al, 2017).…”

Section: Application Of 3d Culture Techniquesmentioning

confidence: 99%

Fabrication techniques of biomimetic scaffolds in three‐dimensional cell culture: A review

Badekila

Kini

Jaiswal

2020

Journal Cellular Physiology

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In the last four decades, several researchers worldwide have routinely and meticulously exercised cell culture experiments in two-dimensional (2D) platforms. Using traditionally existing 2D models, the therapeutic efficacy of drugs has been inappropriately validated due to the failure in generating the precise therapeutic response. Fortunately, a 3D model addresses the foregoing limitations by recapitulating the in vivo environment. In this context, one has to contemplate the design of an appropriate scaffold for favoring the organization of cell microenvironment. Instituting pertinent model on the platter will pave way for a precise mimicking of in vivo conditions. It is because animal cells in scaffolds oblige spontaneous formation of 3D colonies that molecularly, phenotypically, and histologically resemble the native environment. The 3D culture provides insight into the biochemical aspects of cell-cell communication, plasticity, cell division, cytoskeletal reorganization, signaling mechanisms, differentiation, and cell death. Focusing on these criteria, this paper discusses in detail, the diversification of polymeric scaffolds based on their available resources. The paper also reviews the well-founded and latest techniques of scaffold fabrication, and their applications pertaining to tissue engineering, drug screening, and tumor model development.

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“…Moreover, the regeneration of bone tissue was found to be enhanced with 3D spheroids as compared to single cells. As opposed to the classical tissue engineering technique, which uses single cells on biomaterials the 3D strategy initiates robust bone regeneration and accelerates faster mineralization (Figure ) . An interesting study shows the trans‐differentiation of MSCs on Poly(L‐lactic acid)‐ co ‐poly‐(ϵ‐caprolactone)/collagen electrospun scaffolds to generate 3D hepatic constructs.…”

Section: Recent Advancesmentioning

confidence: 99%

Electrospun Fibers for Recruitment and Differentiation of Stem Cells in Regenerative Medicine

Sankar

Sharma

Rath

et al. 2017

Biotechnology Journal

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Electrospinning is a popular technique used to mimic the natural sub-micron features of the native tissue. The ultra-fine fibers provide a favorable extracellular matrix-like environment for regulation of cellular functions. This article summarizes and reviews the current advances in electrospun fiber application and focuses on the novel strategies applied for tissue regeneration and repair. It explores the different factors affecting the attachment and proliferation of mesenchymal stem cells (MSCs) on the electrospun substrates. The influence of different features of electrospun fibers in the differentiation of MSCs into specific lineages (bone, cartilage, tendon/ligament, and nerves) has been elaborated. In addition, the different techniques to mimic the hierarchical features of tissues and its effect on cellular functions are reviewed. Additionally, the new developments like three-dimensional (3D) electrospinning, 3D spheroid double strategy and the comparative analysis of dynamic and static culture on electrospun scaffolds are discussed. With the intricate understanding of the interaction between the cells and the electrospun fiber matrix we can aim to combine the newer strategies to overcome the existing challenges and improve the potential application of electrospun fibers in the field of tissue regeneration and repair.

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Nanoengineered implant as a new platform for regenerative nanomedicine using 3D well-organized human cell spheroids

Cited by 18 publications

References 53 publications

Enhanced Growth of Lapine Anterior Cruciate Ligament-Derived Fibroblasts on Scaffolds Embroidered from Poly(l-lactide-co-ε-caprolactone) and Polylactic Acid Threads Functionalized by Fluorination and Hexamethylene Diisocyanate Cross-Linked Collagen Foams

Enhanced Growth of Lapine Anterior Cruciate Ligament-Derived Fibroblasts on Scaffolds Embroidered from Poly(l-lactide-co-ε-caprolactone) and Polylactic Acid Threads Functionalized by Fluorination and Hexamethylene Diisocyanate Cross-Linked Collagen Foams

Fabrication techniques of biomimetic scaffolds in three‐dimensional cell culture: A review

Electrospun Fibers for Recruitment and Differentiation of Stem Cells in Regenerative Medicine

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