Nanoengineered shear-thinning and bioprintable hydrogel as a versatile platform for biomedical applications

Zandi, Nooshin; Sani, Ehsan Shirzaei; Mostafavi, Ebrahim; Ibrahim, Dina M.; Saleh, Bahram; Shokrgozar, Mohammad Ali; Tamjid, Elnaz; Weiss, Paul S.; Simchi, Abdolreza; Annabi, Nasim

doi:10.1016/j.biomaterials.2020.120476

Cited by 100 publications

(51 citation statements)

References 60 publications

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“…45 The gels showed a high flow consistency index of 47.95, demonstrating strong crosslinking and structural integrity and a power law index of -0.6 which confirmed their shear thinning nature. 46 The gels showed good structural integrity at 25°C as well as at 37°C with a decreasing trend in the storage modulus with increasing temperature. However, at 45°C the storage modulus dropped sharply below the loss modulus indicating loss of structural integrity presumably due to the denaturation of the peptide crosslinks (Figure 4b).…”

Section: Rheological Characterizationmentioning

confidence: 90%

Coiled Coil Crosslinked Alginate Hydrogels Dampen Macrophage-Driven Inflammation

Clapacs

O'Neill

Shrimali

et al. 2021

Preprint

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Alginate hydrogels are widely used for tissue engineering and regenerative medicine due to their excellent biocompatibility. A facile and commonly used strategy to crosslink alginate is the addition of Ca2+ that leads to hydrogelation. However, extracellular Ca2+ is a secondary messenger in activating inflammasome pathways following physical injury or pathogenic insult leading to persistent inflammation and scaffold rejection. Here we present graft copolymers of charge complementary heterodimeric coiled coil (CC) peptides and alginate that undergo supramolecular self-assembly to form Ca2+ free alginate hydrogels. The formation of heterodimeric CCs was confirmed using circular dichroism spectroscopy and scanning electron microscopy revealed a significant difference in pore size between Ca2+ and CC crosslinked gels. The resulting hydrogels were self-supporting and display shear-thinning and shear-recovery properties. In response to lipopolysaccharide (LPS) stimulation, peritoneal macrophages and bone marrow derived dendritic cells cultured in the CC crosslinked gels exhibited a 10-fold reduction in secretion of the proinflammatory cytokine IL-1β compared to Ca2+ crosslinked gels. A similar respose was also observed in vivo upon peritoneal delivery of Ca2+ or CC crosslinked gels. Analysis of peritoneal lavage showed that macrophages in mice injected with Ca2+ crosslinked gels display a more inflammatory phenotype compared to macrophages from mice injected with CC crosslinked gels. These results suggest that CC peptides by virtue of their tunable sequence-structure-function relationship and mild gelation conditions are promising alternative crosslinkers for alginate and other biopolymer scaffolds used in tissue engineering.

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Section: Rheological Characterizationmentioning

confidence: 90%

Coiled Coil Crosslinked Alginate Hydrogels Dampen Macrophage-Driven Inflammation

Clapacs

O'Neill

Shrimali

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…As a next step, since injectable hydrogels have gained increasing amounts of attention in the fields of biomedicine and bioprinting, and in the delivery of drugs, cells, biomolecules, and growth factors due to their minimally invasive delivery method [43], in order to evaluate the propensity of BT13-based hydrogel to recover its initial viscosity after injection, we performed thixotropy tests. In this test, the injection conditions were simulated through a series of constant shear rate tests (see Materials and Methods for further details).…”

Section: Mechanical Properties Of Lupin-derived Hydrogelmentioning

confidence: 99%

Nanostructure, Self-Assembly, Mechanical Properties, and Antioxidant Activity of a Lupin-Derived Peptide Hydrogel

2021

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Naturally occurring food peptides are frequently used in the life sciences due to their beneficial effects through their impact on specific biochemical pathways. Furthermore, they are often leveraged for applications in areas as diverse as bioengineering, medicine, agriculture, and even fashion. However, progress toward understanding their self-assembling properties as functional materials are often hindered by their long aromatic and charged residue-enriched sequences encrypted in the parent protein sequence. In this study, we elucidate the nanostructure and the hierarchical self-assembly propensity of a lupin-derived peptide which belongs to the α-conglutin (11S globulin, legumin-like protein), with a straightforward N-terminal biotinylated oligoglycine tag-based methodology for controlling the nanostructures, biomechanics, and biological features. Extensive characterization was performed via Circular Dichroism (CD) spectroscopy, Fourier Transform Infrared spectroscopy (FT-IR), rheological measurements, and Atomic Force Microscopy (AFM) analyses. By using the biotin tag, we obtained a thixotropic lupin-derived peptide hydrogel (named BT13) with tunable mechanical properties (from 2 to 11 kPa), without impairing its spontaneous formation of β-sheet secondary structures. Lastly, we demonstrated that this hydrogel has antioxidant activity. Altogether, our findings address multiple challenges associated with the development of naturally occurring food peptide-based hydrogels, offering a new tool to both fine tune the mechanical properties and tailor the antioxidant activities, providing new research directions across food chemistry, biochemistry, and bioengineering.

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“…However complex processing and mechanical characterisation techniques has limited use within biological research [ 71 , 92 ]. Fluid gels display similar self-healing properties following displacement, lending both materials to injectable systems, such as bioprinting [ 93 ]. Bioprinting is a bottom-up approach that enables geometrically controlled assembly of complex 3D structures, with inclusion of functional elements producing scaffolds that encompass mechanically and biologically relevant cues [ 69 , 94 ].…”

Section: Biomaterialsmentioning

confidence: 99%

“…Bioinks for neural bioprinting are continually evolving, exploiting iPSC technology and an increased understanding of biochemical features of the CNS, to create specialised formulations capable of recapitulating a myriad of environmental cues [ 71 , 95 , 96 ]. Fluid gels show particular promise as bioinks, protecting cells from shear forces during the printing process that would otherwise reduce viability [ 69 , 93 , 97 ]. 3D bioprinting of hydrogels allows reliable production of spatially defined macrostructures, including vascular components, with the use of sacrificial inks suggested as a means to create complex vascular networks [ 70 , 98 , 99 ].…”

Section: Biomaterialsmentioning

confidence: 99%

Modelling the central nervous system: tissue engineering of the cellular microenvironment

Walczak

Esteban

Bassett

et al. 2021

Emerging Topics in Life Sciences

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With the increasing prevalence of neurodegenerative diseases, improved models of the central nervous system (CNS) will improve our understanding of neurophysiology and pathogenesis, whilst enabling exploration of novel therapeutics. Studies of brain physiology have largely been carried out using in vivo models, ex vivo brain slices or primary cell culture from rodents. Whilst these models have provided great insight into complex interactions between brain cell types, key differences remain between human and rodent brains, such as degree of cortical complexity. Unfortunately, comparative models of human brain tissue are lacking. The development of induced Pluripotent Stem Cells (iPSCs) has accelerated advancement within the field of in vitro tissue modelling. However, despite generating accurate cellular representations of cortical development and disease, two-dimensional (2D) iPSC-derived cultures lack an entire dimension of environmental information on structure, migration, polarity, neuronal circuitry and spatiotemporal organisation of cells. As such, researchers look to tissue engineering in order to develop advanced biomaterials and culture systems capable of providing necessary cues for guiding cell fates, to construct in vitro model systems with increased biological relevance. This review highlights experimental methods for engineering of in vitro culture systems to recapitulate the complexity of the CNS with consideration given to previously unexploited biophysical cues within the cellular microenvironment.

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Nanoengineered shear-thinning and bioprintable hydrogel as a versatile platform for biomedical applications

Cited by 100 publications

References 60 publications

Coiled Coil Crosslinked Alginate Hydrogels Dampen Macrophage-Driven Inflammation

Coiled Coil Crosslinked Alginate Hydrogels Dampen Macrophage-Driven Inflammation

Nanostructure, Self-Assembly, Mechanical Properties, and Antioxidant Activity of a Lupin-Derived Peptide Hydrogel

Modelling the central nervous system: tissue engineering of the cellular microenvironment

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