Nanofiber size-dependent sensitivity of fibroblast directionality to the methodology for scaffold alignment

Chaurey, Vasudha; Block, Frank E.; Su, Yi‐Hsuan; Chiang, Pen‐Chi; Botchwey, Edward A.; Chou, Chia-Fu; Swami, Nathan S.

doi:10.1016/j.actbio.2012.06.041

Cited by 54 publications

(53 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main goal of tissue engineering is to reproduce the topography of the physiological extracellular matrix at the nanoscale, providing to cells an optimal structure for their arrangement. The literature reports the use of microor nanopatterned scaffolds capable to align growing cells helping the wound healing, obtained in the form of membranes [98,99] or fibers [100] by nanoimprinting soft-lithography. As a further improvement of this approach, MI scaffolds can be studied to increase the biomimicry of the device.…”

Section: Membranes: Smart Separation Of Single Molecules and Tissue Engmentioning

confidence: 99%

Molecularly Imprinted Polymeric Micro- and Nano-Particles for the Targeted Delivery of Active Molecules

Gagliardi

Mazzolai

2015

Future Med. Chem.

View full text Add to dashboard Cite

Molecular imprinting (MI) represents a strategy to introduce a 'molecular memory' in a polymeric system obtaining materials with specific recognition properties. MI particles can be used as drug delivery systems providing a targeted release and thus reducing the side effects. The introduction of molecular recognition properties on a polymeric drug carrier represents a challenge in the development of targeted delivery systems to increase their efficiency. This review will summarize the limited number of drug delivery MI particles described in the literature along with an overview of potential solutions for a larger exploitation of MI particles as targeted drug delivery carriers. Molecularly imprinted drug carriers can be considered interesting candidates to significantly improve the efficiency of a controlled drug treatment.

show abstract

Section: Membranes: Smart Separation Of Single Molecules and Tissue Engmentioning

confidence: 99%

Molecularly Imprinted Polymeric Micro- and Nano-Particles for the Targeted Delivery of Active Molecules

Gagliardi

Mazzolai

2015

Future Med. Chem.

View full text Add to dashboard Cite

show abstract

“…Recently, some properties of the 3D bioengineered scaffold including strength, porosity, and configuration have been examined to improve diverse ECM platforms . In particular, there have been numerous reports on cell growth and related behavior of 3D scaffolds which possessed aligned biotopographical cues due to the regular organization of most of the structures of native tissues . For example, skeletal muscle tissue is composed of aligned myofibers, and blood vessel is made up of aligned vascular endothelial cells along the axial direction.…”

Section: Introductionmentioning

confidence: 99%

Controllable Aligned Nanofiber Hybrid Yarns with Enhanced Bioproperties for Tissue Engineering

Liu

Mao

et al. 2019

Macro Materials & Eng

View full text Add to dashboard Cite

Electrospun nanofibers have large surface area, high porosity, and controllable orientation while conventional microfibers have appropriate mechanical properties such as stiffness, strength, and elasticity. Therefore, the combination of nanofibers and microfibers can provide building elements to engineer biomimetic scaffolds for tissue engineering. In this study, a core–shell structured fibrous structure with controllable surface topography is created by electrospinning polycaprolactone (PCL) nanofibers onto polyglycolic acid (PGA) microfibers. The surface morphology, surface wettability, and mechanical properties of the resultant core–shell structure are characterized. FE‐SEM images reveal that the orientation of PCL nanofibers on the yarn surface can be tuned by a fiber collector and rotating disks. Benefiting from the introduction of a shell of aligned PCL nanofibers on the core of PGA yarn, the uniaxially aligned PCL nanofiber–covered yarns (A‐PCLs) exhibit higher hydrophilicity, porosity, and mechanical properties than the core PGA yarns. Moreover, A‐PCLs promote the adhesion and proliferation of BALB/3T3 (mouse embryonic fibroblast cell line), and guide cell growth along the biotopographic cues of the PCL nanofibers with controllable alignment. The developed core–shell yarn having both the desired surface topography of PCL nanofibers and mechanical properties of PGA microfibers demonstrates great potential in constructing various tissue scaffolds.

show abstract

“…The topography of the environment has been also identified as a potent guidance cue for growing axons and migrating cells through a process called contact guidance [41,42]. To dissect out the role of such physical cues, several engineered topographical substrates, such as grooves [43,44], pillars [45,46,47], pits [48,49] or synthetic fibers [50,51], were used to demonstrate in vitro the influence of topography on the migration of different cell types. A few studies employed micro-channels as a culture system for migrating cortical interneurons [52,53].…”

Section: Introductionmentioning

confidence: 99%

Topographical cuescontrol the morphology and dynamics of migrating cortical interneurons

Leclech

Renner

Villard

et al. 2018

Preprint

View full text Add to dashboard Cite

In mammalian embryos, cortical interneurons travel long distances among complex threedimensional tissues before integrating into cortical circuits. Several molecular guiding cues involved in this migration process have been identified, but the influence of physical parameters remains poorly understood. In the present study, we have investigated in vitro the influence of the topography of the microenvironment on the migration of primary cortical interneurons released from mouse embryonic explants.We found that arrays of 10 µm-sized PDMS micro-pillars, either round or square, influenced both the morphology and the migratory behavior of interneurons. Strikingly, most interneurons exhibited a single and long leading process oriented along the diagonals of the square pillared array, whereas leading processes of interneurons migrating in-between round pillars were shorter, often branched and oriented in all available directions. Accordingly, dynamic studies revealed that growth cone divisions were twice more frequent in round than in square pillars. Both soma and leading process tips presented forward directed movements within square pillars, contrasting with the erratic trajectories and more dynamic movements observed among round pillars. In support of these observations, long interneurons migrating in square pillars displayed tight bundles of stable microtubules aligned in the direction of migration.Overall, our results show that micron-sized topography provides global spatial constraints promoting the establishment of two different morphological and migratory states.Remarkably, both states belong to the natural range of migratory behaviors of cortical interneurons, highlighting the potential importance of topographical cues in the guidance of these embryonic neurons, and more generally in brain development.In this study, we investigated this question using microstructured substrates and demonstrated the high sensitivity of cortical interneurons to the architecture of their environment. Our results revealed that topographical constraints efficiently modulate the migratory behavior of interneurons by controlling cell morphology, branching dynamics and cytoskeletal organization. In particular, by using two shapes of micron-sized pillars, we have been able to select and stabilize in vitro two main migratory behaviors observed in vivo, associated with specific morphologies: non-branched cells with long leading processes showed a directional movement among square pillars, and branched cells with short leading processes displayed a fast, exploratory migration among round pillars. This study therefore provides new insights into the guidance and the biology of these cells. The in vitro model described in this study thus emerges as a new promising tool to control and study interneuron migratory behaviors in physiological or pathological conditions.

show abstract

Nanofiber size-dependent sensitivity of fibroblast directionality to the methodology for scaffold alignment

Cited by 54 publications

References 30 publications

Molecularly Imprinted Polymeric Micro- and Nano-Particles for the Targeted Delivery of Active Molecules

Molecularly Imprinted Polymeric Micro- and Nano-Particles for the Targeted Delivery of Active Molecules

Controllable Aligned Nanofiber Hybrid Yarns with Enhanced Bioproperties for Tissue Engineering

Topographical cuescontrol the morphology and dynamics of migrating cortical interneurons

Contact Info

Product

Resources

About