Nanofiber technology: Designing the next generation of tissue engineering scaffolds

Barnes, Catherine P.; Sell, Scott A.; Boland, Eugene D.; Simpson, David G.; Bowlin, Gary L.

doi:10.1016/j.addr.2007.04.022

Cited by 1,038 publications

(789 citation statements)

References 64 publications

Supporting

Mentioning

758

Contrasting

Unclassified

Order By: Relevance

“…ECM is a dynamic 3D microenvironment, where signals are transmitted between cells and ECM, which enables cell communication, adhesion, proliferation and differentiation. Bone matrix forms a nanometer‐sized composite of organic and inorganic materials with proteins in the range of 50–500 nm 30, 31. Electrospinning has been widely used to fabricate nanoscale substrates 10, 11, 12, 17, 18, 22, 28.…”

Section: Discussionmentioning

confidence: 99%

Hydrostatic pressure in combination with topographical cues affects the fate of bone marrow‐derived human mesenchymal stem cells for bone tissue regeneration

Reinwald

Haj

2017

J Biomedical Materials Res

View full text Add to dashboard Cite

Topographical and mechanical cues are vital for cell fate, tissue development in vivo, and to mimic the native cell growth environment in vitro. To date, the combinatory effect of mechanical and topographical cues as not been thoroughly investigated. This study investigates the effect of PCL nanofiber alignment and hydrostatic pressure on stem cell differentiation for bone tissue regeneration. Bone marrow‐derived human mesenchymal stem cells were seeded onto standard tissue culture plastic and electrospun random and aligned nanofibers. These substrates were either cultured statically or subjected to intermittent hydrostatic pressure at 270 kPa, 1 Hz for 60 min daily over 21 days in osteogenic medium. Data revealed higher cell metabolic activities for all mechanically stimulated cell culture formats compared with non‐stimulated controls; and random fibers compared with aligned fibers. Fiber orientation influenced cell morphology and patterns of calcium deposition. Significant up‐regulation of Collagen‐I, ALP, and Runx‐2 were observed for random and aligned fibers following mechanical stimulation; highest levels of osteogenic markers were expressed when hydrostatic pressure was applied to random fibers. These results indicate that fiber alignment and hydrostatic pressure direct stem cell fate and are important stimulus for tissue regeneration. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: A: 629–640, 2018.

show abstract

Section: Discussionmentioning

confidence: 99%

Hydrostatic pressure in combination with topographical cues affects the fate of bone marrow‐derived human mesenchymal stem cells for bone tissue regeneration

Reinwald

Haj

2017

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…1 In the last few years, the electrospinning technique has been recognized as an efficient technology, compared to others such as self-assembly, 2 phase separation, 3 drawing, 4 and template-directed synthesis, 5 that allows creation of polymer nanofibers, in the form of nonwoven mats, and that can be scaled up from laboratory to industrial production. In biomedical engineering, electrospun nanofibers exhibit a lot of advantages, particularly to produce scaffolds whose physicochemical properties can closely resemble the naturally occurring properties of the extracellular matrix (ECM) components found in tissues.…”

Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone)/keratin-based composite nanofibers for biomedical applications

Edwards

Jarvis

Hopkins

et al. 2014

J. Biomed. Mater. Res.

114

View full text Add to dashboard Cite

Keratin-based composite nanofibers have been fabricated by an electrospinning technique. Aqueous soluble keratin extracted from human hair was successfully blended with poly(e-caprolactone) (PCL) in different ratios and transformed into nanofibrous membranes. Toward the potential use of this nanofibrous membrane in tissue engineering, its physicochemical properties, such as morphology, mechanical strength, crystallinity, chemical structure, and integrity in aqueous medium were studied and its cellular compatibility was determined. Nanofibrous membranes with PCL/keratin ratios from 100/00 to 70/30 showed good uniformity in fiber morphology and suitable mechanical properties, and retained the integrity of their fibrous structure in buffered solutions.Experimental results, using cell viability assays and scanning electron microscopy imaging, showed that the nanofibrous membranes supported 3T3 cell viability. The ability to produce blended nanofibers from protein and synthetic polymers represents a significant advancement in development of composite materials with structural and material properties that will support biomedical applications. This provides new nanofibrous materials for applications in tissue engineering and regenerative medicine.

show abstract

“…One approach is to blend proteins with other natural and synthetic polymers, which are more water stable. For example, collagen was blended with poly(ethylene oxide) (PEO) [19] or polydioxanone (POD) [20] to produce electrospun fibers. The collagen-PEO/PDO fiber blends displayed improved mechanical properties and better shape retention.…”

Section: Introductionmentioning

confidence: 99%

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

2010

View full text Add to dashboard Cite

Nanofiber technology: Designing the next generation of tissue engineering scaffolds

Cited by 1,038 publications

References 64 publications

Hydrostatic pressure in combination with topographical cues affects the fate of bone marrow‐derived human mesenchymal stem cells for bone tissue regeneration

Hydrostatic pressure in combination with topographical cues affects the fate of bone marrow‐derived human mesenchymal stem cells for bone tissue regeneration

Poly(ε-caprolactone)/keratin-based composite nanofibers for biomedical applications

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

Contact Info

Product

Resources

About