Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells

Liu, Li; Yoshioka, Mieko; Nakajima, Minoru; Ogasawara, Arata; Li, Jun; Hasegawa, Kouichi; Li, Sisi; Zou, Jianli; Nakatsuji, Norio; Kamei, Ken‐ichiro; Chen, Yong

doi:10.1016/j.biomaterials.2014.04.024

Cited by 53 publications

(60 citation statements)

References 34 publications

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“…The gelatin nanofibers were electrospun on the PEGDA frame 6 . For more uniform collection of nanofibers, the PEGDA frame was coated with a 10 nm thick Au and placed on a silicon wafer as collector.…”

Section: Fabrication Of the Culture Patchmentioning

confidence: 99%

Induction and differentiation of human induced pluripotent stem cells into functional cardiomyocytes on a compartmented monolayer of gelatin nanofibers

Tang

Liu

et al. 2016

Nanoscale

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Extensive efforts have been devoted to develop new substrates for culture and differentiation of human induced pluripotent stem cells (hiPSCs) toward cardiac cell-based assays. A more exciting prospect is the construction of cardiac tissue for robust drug screening and cardiac tissue repairing. Here, we developed a patch method by electrospinning and crosslinking of monolayer gelatin nanofibers on a honeycomb frame made of poly(ethylene glycol) diacrylate (PEGDA). The monolayer of the nanofibrous structure can support cells with minimal exogenous contact and a maximal efficiency of cell-medium exchange whereas a single hiPSC colony can be uniformly formed in each of the honeycomb compartments. By modulating the treatment time of the ROCK inhibitor Y-27632, the shape of the hiPSC colony could be controlled from a flat layer to a hemisphere. Afterwards, the induction and differentiation of hiPSCs were achieved on the same patch, leading to a uniform cardiac layer with homogeneous contraction. This cardiac layer could then be used for extracellular recording with a commercial multi-electrode array, showing representative field potential waveforms of matured cardiac tissues with appropriate drug responses.

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Section: Fabrication Of the Culture Patchmentioning

confidence: 99%

Induction and differentiation of human induced pluripotent stem cells into functional cardiomyocytes on a compartmented monolayer of gelatin nanofibers

Tang

Liu

et al. 2016

Nanoscale

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“…On the other hand, natural bio-polymers such as gelatin, which is produced by hydrolyzing collagen, should be more relevant than synthetic polymers in terms of biocompatibility [13]. Previously, gelatin nanofibers could be used for long term expansion of human induced pluripotent stem cells (hiPSCs) [14]. Most recently, we proposed a culture patch method by using monolayer gelatin nanofibers for hiPSCs differentiation towards mature motor neurons and cardiomyocytes [15,16].…”

Section: Introductionmentioning

confidence: 99%

Patch method for culture of primary hippocampal neurons

Tang

Severino

Iseppon

et al. 2017

Microelectronic Engineering

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In vitro culture of primary neurons, especially hippocampal neurons, is important for understanding cellular mechanisms in neurobiology. Actually, this is achieved by using culture dish or glass slide with surface coated proteins. Here, we proposed a patch method to culture primary neurons on a monolayer of gelatin nanofibers, electrospun and crosslinked on a microfabricated honeycomb frame of poly (ethylene glycol) diacrylate (PEGDA). By using such a patch method, neural networks could be formed with a minimal cell-exogenous materials contact and a maximal exposure of the cells to the medium. Interestingly, hippocampal cells, especially astrocytes, showed in-vivo like morphology and most of neurons were found in the porous areas inside the honeycomb compartments although the nanofibers were deposited everywhere of the frame. Finally, calcium imaging showed that primary neurons have a higher degree of neural activity on the patch than on glass.

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“…The nanofiber size ranged from 70 to 500 nm by altering the gelatin concentration while the gelatin density ranged from 0.1 to 5.8 lg/cm 2 by changing the amount of gelatin incorporated. Results revealed that the nanofiber with intermediate size (240 nm) and high gelatin density (4.8 lg/cm 2 ) was optimal in cultivating human ESCs (H9 and H1) for long-term periods without sacrificing a self-renewal capacity [194] (Fig. 4.16c and d).…”

Section: D Geometrical Scaffoldsmentioning

confidence: 94%

“…Table 4.1 summarizes the representative biomaterials that have been used to culture PSCs that maintain the pluripotency and self-renewal capacity of the PSCs. [191,194]. …”

Section: D Geometrical Scaffoldsmentioning

confidence: 98%