Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells

Vocetková, Karolína; Buzgo, Matěj; Sovková, Věra; Bezděková, Dagmar; Kneppo, Peter; Amler, Evžen

doi:10.1111/cpr.12276

Cited by 25 publications

(28 citation statements)

References 37 publications

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“…According to the data, PVA nanofibers are not suitable for cell culture regardless of their functionalization with platelets. The cell adhesion properties of PVA nanofibers could be improved using other polymers, such as chitosan [20], gelatin [21] or hyaluronic acid [22]. On the other hand, PCL was shown to be effective in fostering the culture of melanocyte, which is in accordance with previously published data [14,18].…”

Section: Discussionsupporting

confidence: 88%

Functionalization of Polymeric Nanofibers Using Platelets for Melanocyte Culture

et al. 2020

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Tissue engineering is an interdisciplinary field that uses a combination of cells, suitable biomaterials and bioactive molecules to engineer the desired tissue and restore lost function. These principles have quickly begun to spread to the therapy of multiple diseases, including depigmentation disorders. The most common depigmentation disorder is vitiligo, a disease with deep psychosocial implications. Thanks to their unique properties, electrospun polymeric nanofibers represent a material suitable for tissue engineering applications. Furthermore, they may be functionalized with platelets, cells that contain a wide spectrum of growth factors and chemokines. The aim of this paper was to evaluate the functionalization of polymeric nanofibers with platelets and their effects in melanocyte culture. The scaffolds were visualized using scanning electron microscopy, the metabolic activity and proliferation of melanocytes was determined using MTS assay and dsDNA quantification, respectively. Furthermore, the melanocytes were stained and visualized using confocal microscopy. The acquired data showed that poly-ε-caprolactone functionalized with platelets promoted the viability and proliferation of melanocytes. According to the results, such a functionalized scaffold combining nanofibers and platelets may be suitable for melanocyte culture.

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Section: Discussionsupporting

confidence: 88%

Functionalization of Polymeric Nanofibers Using Platelets for Melanocyte Culture

et al. 2020

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“…The performed SEM analysis demonstrated the presence of a fine fibrin network on the surface of the fibrous samples, functionalized with the highest concentrations of platelets. A similar fibrin network was observed in other studies, evaluating the positive effect of combining platelets and nanofibrous scaffolds [ 25 , 44 , 45 ]. It has been shown that the structure of the fibrin network, in terms of diameter, branching and density, may be influenced by various factors including pH and calcium, thrombin or fibrinogen concentration [ 46 ].…”

Section: Discussionsupporting

confidence: 85%

“…The best results were obtained in the centrifugally spun samples with the highest concentration of platelets (the highest tested concentration of platelet-derived molecules, C10). Nanofibers made of PCL, using electrospinning, were successfully used for the melanocyte culture [ 45 , 57 , 58 ]. Savkovic et al showed PCL nanofibers to be superior to a 2D cell culture substrate, in terms of melanin synthesis [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…Savkovic et al showed PCL nanofibers to be superior to a 2D cell culture substrate, in terms of melanin synthesis [ 57 ]. The functionalization of PCL nanofibers with platelets led to improvements of cell metabolic activity and proliferation, not only in melanocytes but also in keratinocytes and fibroblasts [ 45 ]. Such pleotropic effects of PRP may be very useful in vitiligo therapy, as keratinocytes and fibroblasts were also implicated in its etiopathogenesis [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

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A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment

et al. 2020

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Vitiligo is the most common depigmentation disorder of the skin. Currently, its therapy focuses on the halting of the immune response and stimulation of the regenerative processes, leading to the restoration of normal melanocyte function. Platelet-rich plasma (PRP) represents a safe and cheap regenerative therapy option, as it delivers a wide spectrum of native growth factors, cytokines and other bioactive molecules. The aim of this study was to develop a simple delivery system to prolong the effects of the bioactive molecules released from platelets. The surface of electrospun and centrifugally spun poly-ε-caprolactone (PCL) fibrous scaffolds was functionalized with various concentrations of platelets; the influence of the morphology of the scaffolds and the concentration of the released platelet-derived bioactive molecules on melanocytes, was then assessed. An almost two-fold increase in the amount of the released bioactive molecules was detected on the centrifugally spun vs. electrospun scaffolds, and a sustained 14-day release of the bioactive molecules was demonstrated. A strong concentration-dependent response of melanocyte to the bioactive molecules was observed; higher concentrations of bioactive molecules resulted in improved metabolic activity and proliferation of melanocytes. This simple system improves melanocyte viability, offers on-site preparation and is suitable for prolonged topical PRP administration.

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“…The emulsion of PF-68 in PCL reduced the surface tension of the polymeric solution and led to improved electrospinning efficiency. The needleless emulsion electrospinning resulted in the formation of nanofibres with nano−/micro-morphology typical for PCL electrospun from a chloroform/ethanol solvent system (Plencner et al, 2014;Vocetkova et al, 2016). The nano−/ micro-morphology was shown as a suitable morphology for the cultivation of chondrocytes (Jakubova et al, 2011) and fibroblasts (Plencner et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Needleless emulsion electrospinning for the regulated delivery of susceptible proteins

Buzgo

Filová

Staffa

et al. 2017

J Tissue Eng Regen Med

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In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process. The nanofibres are based on poly-ε-caprolactone (PCL) as a continuous phase combined with a droplet phase based on Pluronic F-68 (PF-68). The PCL-PF-68 nanofibres show a time-regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5-(4,6-dichlorotriazinyl) aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor-β and basic fibroblast growth factor]. In addition, the PF-68 facilitates the preservation of the bioactivity of delivered proteins. The system's potential was highlighted by an improvement in the metabolic activity and proliferation of mesenchymal stem cells. The developed system has the potential to deliver susceptible molecules in tissue-engineering applications.

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Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells

Abstract: Such complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but also in broader applications in regenerative medicine.

Cited by 25 publications

References 37 publications

Functionalization of Polymeric Nanofibers Using Platelets for Melanocyte Culture

Functionalization of Polymeric Nanofibers Using Platelets for Melanocyte Culture

A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment

Needleless emulsion electrospinning for the regulated delivery of susceptible proteins

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