Nanoflow liquid chromatography mass spectrometry method for quantitative analysis and target ion screening of pyrrolizidine alkaloids in honey, tea, herbal tinctures, and milk

Jansons, Mārtiņš; Fedorenko, Deniss; Pavlenko, Romans; Berzina, Zane; Bartkevičs, Vadims

doi:10.1016/j.chroma.2022.463269

Cited by 17 publications

(10 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The solvents used were LLE and SALLE, and the clean-up sorbents included anhydrous magnesium sulfate (MgSO 4 ), sodium chloride (NaCl), and primary-secondary amine (PSA). A QuEChERS-based extraction procedure involving acetonitrile, NaCl, MgSO 4 , sodium citrate, and sodium citrate tribasic clean-up sorbents has been applied for the extraction and analysis of PAs/PANOs in honey and oregano [39,56]. Izcara et al (2020) presented the development of a miniaturized strategy based on the QuEChERS method combined with UHPLC-MS/MS for the determination of 21 PAs suggested by the European Food Safety Authority.…”

Section: Pas Extraction and Detection Methodsmentioning

confidence: 99%

Levels, Toxic Effects, and Risk Assessment of Pyrrolizidine Alkaloids in Foods: A Review

Lu,

Qiu,

et al. 2024

Foods

View full text Add to dashboard Cite

Pyrrolizidine alkaloids (PAs) are naturally occurring secondary metabolites of plants. To date, more than 660 types of PAs have been identified from an estimated 6000 plants, and approximately 120 of these PAs are hepatotoxic. As a result of PAs being found in spices, herbal teas, honey, and milk, PAs are considered contaminants in foods, posing a potential risk to human health. Here, we summarize the chemical structure, toxic effects, levels, and regulation of PAs in different countries to provide a better understanding of their toxicity and risk assessment. With recent research on the risk assessment of PAs, this review also discusses the challenges facing this field, aiming to provide a scientific basis for PA toxicity research and safety assessment.

show abstract

Section: Pas Extraction and Detection Methodsmentioning

confidence: 99%

Levels, Toxic Effects, and Risk Assessment of Pyrrolizidine Alkaloids in Foods: A Review

Lu,

Qiu,

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

“…An unstable electrospray leads to decreased signal intensities and droplet formation at the emitter tip, which affects the sensitivity and applicability of the method. Several approaches to improve nano-electrospray stability have been described, such as post-column solvent addition [27,28], where a highly organic solvent is continuously added to the column eluate via a T-piece to reduce the aqueous phase content and facilitate electrospray, voltage regulation during analysis [29], and selection of the correct nano-ESI voltage. In this work, optimal nano-electrospray performance was achieved by manually selecting a suitable voltage in a range of 1.70-2.50 kV for both positive and negative modes under initial gradient conditions, and the optimal voltages that provided the most stable nano-electrospray were used.…”

Section: Optimisation Of the Orbitrap Ms Parametersmentioning

confidence: 99%

Dilute‐and‐shoot approach for determination of several biomarkers and pharmaceuticals in wastewater using nanoflow liquid chromatography – Orbitrap mass spectrometry

Fedorenko

Podjava

Prikulis

et al. 2023

J of Separation Science

Self Cite

View full text Add to dashboard Cite

A new method for quantitative analysis of several biomarkers and pharmaceutical compounds in wastewater has been developed employing nanoflow liquid chromatography with Orbitrap mass spectrometry. An easy dilute‐and‐shoot approach has been used for sample preparation with a dilution factor of 5. Improved retention of ionic and highly polar compounds has been achieved by the addition of tetrabutylammonium bromide as an ion pair reagent into the final diluted sample. The new nanoflow liquid chromatography method has demonstrated low matrix effects (70%–111%), high sensitivity in terms of limits of quantification (0.005 to 0.3 μg/L), low injection volume (70 nl) and solvent consumption, and the ability to analyze diverse polar and ionic analytes within one run using a single reversed‐phase nanoflow liquid chromatography column. Wastewater samples (n = 116) from the wastewater treatment plants of different cities in Latvia were analyzed using the developed method. The observed concentrations of biomarkers were in line with the literature data.

show abstract

“…It is essential to define national food resource characteristics. First, we must be aware of whether the composition comes with health benefits [49] or risks [50]. Second, product screening at the national level is significant if the characteristics are outstanding in the international context; put simply-if the food is outstanding.…”

Section: Polyfloral Honey Element Chemical Profilementioning

confidence: 99%

Multi-Element Profile Characterization of Monofloral and Polyfloral Honey from Latvia

Labsvards,

Rudovica,

Borisova

et al. 2023

Foods

View full text Add to dashboard Cite

Honey is of scientific interest mainly due to its health-promoting and antibacterial properties, which are also associated with its floral origins. However, the methods for confirming honey floral origins are quite limited and require improvements. One method suggested in the search for a multi-method approach to evaluating the floral origins of Latvian honey is inductively coupled plasma mass spectrometry (ICP-MS). This study investigated the multi-element profile of 83 honey samples of well-specified floral origins. The main findings included using Ba, Ca, Cs, Fe, and Rb as indicator elements for heather honey. The chemometric evaluation supported the use of ICP-MS for distinguishing heather honey from other types of honey. The Latvian polyfloral honey multi-element profile was defined and compared to honey samples with other geographical origins. Additionally, the multi-element profiles of buckwheat, clover, and polyfloral honey proteins were investigated to clarify whether the majority of elements were bound with proteins or not. Preliminary results indicated that Ca, K, Mg, Mn, Na, and Sr were mainly found in non-protein-bound forms, while the majority of Al, Cu, Ni, and Zn were in the form of large chemical structures (>10 kDa).

show abstract

Nanoflow liquid chromatography mass spectrometry method for quantitative analysis and target ion screening of pyrrolizidine alkaloids in honey, tea, herbal tinctures, and milk

Cited by 17 publications

References 23 publications

Levels, Toxic Effects, and Risk Assessment of Pyrrolizidine Alkaloids in Foods: A Review

Levels, Toxic Effects, and Risk Assessment of Pyrrolizidine Alkaloids in Foods: A Review

Dilute‐and‐shoot approach for determination of several biomarkers and pharmaceuticals in wastewater using nanoflow liquid chromatography – Orbitrap mass spectrometry

Multi-Element Profile Characterization of Monofloral and Polyfloral Honey from Latvia

Contact Info

Product

Resources

About