Nanoformulated copper/zinc superoxide dismutase attenuates vascular cell activation and aortic inflammation in obesity

Saraswathi, Viswanathan; Ganesan, Murali; Perriotte-Olson, Curtis; Manickam, Devika S.; Westwood, Rachel A.; Zimmerman, Matthew C.; Ahmad, Iman M.; Desouza, Cyrus; Kabanov, Alexander V.

doi:10.1016/j.bbrc.2015.12.027

Cited by 19 publications

(8 citation statements)

References 38 publications

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“…We previously reported that Nano preferentially delivers SOD1 to endothelial cells compared to native unmodified SOD1 [29]. To determine whether Nano effectively delivers SOD1 to myocytes, we performed in vitro studies in cultured C2C12 myocytes.…”

Section: Resultsmentioning

confidence: 99%

Nanoformulated copper/zinc superoxide dismutase exerts differential effects on glucose vs lipid homeostasis depending on the diet composition possibly via altered AMPK signaling

Gopalakrishnan

Perriotte-Olson

Bhinderwala

et al. 2017

Translational Research

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Evidence suggests that superoxide dismutase 1 (SOD1) promotes glucose versus lipid metabolism depending on the diet type. We recently reported that nanoformulated SOD1 (Nano) improved lipid metabolism without altering glucose homeostasis in high fat (HF) diet-fed mice. Here, we sought to determine the effects and potential mechanisms of Nano in modulating glucose and lipid homeostasis in mice fed a normal chow diet (CD) versus HF diet. Mice were fed a CD or a HF diet (45%) for 10 wk and injected with Nano once every two days for fifteen days. The fasting glucose level was lower (P<0.05) in CD+Nano-treated mice compared to control. Conversely, blood glucose was not altered but serum triglycerides were lower in HF+Nano-treated mice. Genes involved in fatty acid synthesis were reduced by Nano in the skeletal muscle of CD but not of HF diet-fed mice. AMPK, which promotes both glucose and lipid metabolism depending on the fuel availability, is activated by Nano in CD-fed mice. Moreover, Nano increased phosphorylation of ACC, a downstream target of AMPK, in both CD and HF diet-fed mice. Nano increased mitochondrial respiration in C2C12 myocytes in the presence of glucose or fatty acid and this effect is inhibited by Compound C, an AMPK inhibitor. Our data suggest that Nano promotes glucose and lipid metabolism in CD and HF diet-fed mice, respectively, and this effect is mediated partly via AMPK signaling.

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Section: Resultsmentioning

confidence: 99%

Nanoformulated copper/zinc superoxide dismutase exerts differential effects on glucose vs lipid homeostasis depending on the diet composition possibly via altered AMPK signaling

Gopalakrishnan

Perriotte-Olson

Bhinderwala

et al. 2017

Translational Research

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“…The antioxidant effect of SOD at the cardiovascular level has also been demonstrated in human aortic endothelial cells (HAEC), in which nano-SOD decreased linoleic acid-induced oxidative stress, as demonstrated by the in vivo assessment of nano-SOD in vascular-cell activation in a mouse model of diet-induced obesity. Nano-SOD caused a significant decrease in vascular-cell activation in the thoracic aorta, in heart inflammation and in MMP expression in the aorta and ventricles [ 191 ].…”

Section: Sod As a Pharmacological Agentmentioning

confidence: 99%

Superoxide Dismutase Administration: A Review of Proposed Human Uses

et al. 2021

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Superoxide dismutases (SODs) are metalloenzymes that play a major role in antioxidant defense against oxidative stress in the body. SOD supplementation may therefore trigger the endogenous antioxidant machinery for the neutralization of free-radical excess and be used in a variety of pathological settings. This paper aimed to provide an extensive review of the possible uses of SODs in a range of pathological settings, as well as describe the current pitfalls and the delivery strategies that are in development to solve bioavailability issues. We carried out a PubMed query, using the keywords “SOD”, “SOD mimetics”, “SOD supplementation”, which included papers published in the English language, between 2012 and 2020, on the potential therapeutic applications of SODs, including detoxification strategies. As highlighted in this paper, it can be argued that the generic antioxidant effects of SODs are beneficial under all tested conditions, from ocular and cardiovascular diseases to neurodegenerative disorders and metabolic diseases, including diabetes and its complications and obesity. However, it must be underlined that clinical evidence for its efficacy is limited and consequently, this efficacy is currently far from being demonstrated.

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“…In particular, Zn supplementation significantly increased GPx activity through modulation of Se status [ 53 ]. It has also been demonstrated that nanoformulated Cu,Zn-SOD is capable of decreasing adipose tissue [ 54 ] and vascular [ 55 ] inflammation in obese mice.…”

Section: Introductionmentioning

confidence: 99%

Zinc status is associated with inflammation, oxidative stress, lipid, and glucose metabolism

et al. 2017

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A number of studies have reported that zinc plays a substantial role in the development of metabolic syndrome, taking part in the regulation of cytokine expression, suppressing inflammation, and is also required to activate antioxidant enzymes that scavenge reactive oxygen species, reducing oxidative stress. Zinc also plays a role in the correct functioning of lipid and glucose metabolism, regulating and forming the expression of insulin. In numerous studies, zinc supplementation has been found to improve blood pressure, glucose, and LDL cholesterol serum level. Deeper knowledge of zinc’s properties may help in treating metabolic syndrome, thus protecting against stroke and angina pectoris, and ultimately against death.

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Nanoformulated copper/zinc superoxide dismutase attenuates vascular cell activation and aortic inflammation in obesity

Cited by 19 publications

References 38 publications

Nanoformulated copper/zinc superoxide dismutase exerts differential effects on glucose vs lipid homeostasis depending on the diet composition possibly via altered AMPK signaling

Nanoformulated copper/zinc superoxide dismutase exerts differential effects on glucose vs lipid homeostasis depending on the diet composition possibly via altered AMPK signaling

Superoxide Dismutase Administration: A Review of Proposed Human Uses

Zinc status is associated with inflammation, oxidative stress, lipid, and glucose metabolism

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