Nanogel-quantum dot hybrid nanoparticles for live cell imaging

Hasegawa, Urara; Nomura, Shin Ichiro M.; Kaul, Sunil C.; Hirano, Takashi; Akiyoshi, Kazunari

doi:10.1016/j.bbrc.2005.03.228

Cited by 201 publications

(115 citation statements)

References 27 publications

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“…1(a)) which is used extensively as a food additive, and has therefore been shown to be safe for human use. However, recently pullulan has also been investigated for its biomedical applications in areas such as targeted drug and gene delivery and diagnostic imaging using quantum dots [15,16], for the following reasons: (1) it is non-toxic, (2) its usefulness as a plasma expander, (3) its non-immunogenic properties, and (4) its non-antigenic properties. The pullulan modified magnetic nanoparticles reported previously in the literature were prepared by adding an aqueous solution of pullulan to magnetite particles dispersed in deoxygenated water [17].…”

Section: Introductionmentioning

confidence: 99%

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

et al. 2010

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Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity and cellular uptake of the magnetic nanoparticles were examined. The hyperthermic effect of the magnetic nanoparticles on tumor cells was evaluated. Transmission electron microscopy (TEM) showed that the PA coated magnetic nanoparticles (PAMNs) had spherical morphology. Dynamic light scattering (DLS) showed that the size distribution of PAMNs was unimodal,with an average diameter of 25.8 nm ± 6.1 nm. The presence of the adsorbed layer of PA on the magnetite surface was confirmed by Fourier transform infrared (FTIR) spectroscopy. Magnetic measurements revealed that the saturation magnetization of the PAMNs reached 51.9 emu/g and the nanoparticles were superparamagnetic. Thermogravimetric analysis (TGA) showed that the Fe 3 O 4 particles constituted 75 wt% of the PAMNs. The PAMNs had good heating properties in an alternating magnetic field. Cytotoxicity assay showed that PAMNs exhibited no significant cytotoxicity against L929 cells. TEM results showed that a large number of PAMNs were internalized into KB cells. PAMNs have good hyperthermia effect on KB cells in vitro by magnetic field induced hyperthermia. These novel magnetic nanoparticles have great potential as magnetic hyperthermia mediators.

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Section: Introductionmentioning

confidence: 99%

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

et al. 2010

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“…Pullulan (MW = 1.0 · 10 5 ) was substituted with 1.4 cholesterol moieties (CHP). CHPNH 2 was synthesized by conjugation of ethylene diamine with CHP [23]. CHPNH 2 with 12 amino groups per 100 glucose units was used in this study.…”

Section: Nanogelsmentioning

confidence: 99%

Inhibition of the formation of amyloid β‐protein fibrils using biocompatible nanogels as artificial chaperones

et al. 2006

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The formation of fibrils by amyloid b-protein (Ab) is considered as a key step in the pathology of Alzheimer's disease (AD). Inhibiting the aggregation of Ab is a promising approach for AD therapy. In this study, we used biocompatible nanogels composed of a polysaccharide pullulan backbone with hydrophobic cholesterol moieties (cholesterol-bearing pullulan, CHP) as artificial chaperones to inhibit the formation of Ab-(1-42) fibrils with marked amyloidgenic activity and cytotoxicity. The CHPnanogels incorporated up to 6-8 Ab-(1-42) molecules per particle and induced a change in the conformation of Ab from a random coil to a-helix-or b-sheet-rich structure. This structure was stable even after a 24-h incubation at 37°C and the aggregation of Ab-(1-42) was suppressed. Furthermore, the dissociation of the nanogels caused by the addition of methylb-cyclodextrin released monomeric Ab molecules. Nanogels composed of amino-group-modified CHP (CHPNH 2 ) with positive charges under physiological conditions had a greater inhibitory effect than CHP-nanogels, suggesting the importance of electrostatic interactions between CHPNH 2 and Ab for inhibiting the formation of fibrils. In addition, CHPNH 2 nanogels protected PC12 cells from Ab toxicity.

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“…Pullulan is biodegradable, impermeable to oxygen, nonhygroscopic, and nonreducing. Recently, the role of polysaccacharides in developing controlled drug delivery systems has increased significantly, and pullulan is gaining lot of attraction for this application [111]. Tissue engineering requires scaffolds or artificial extracellular matrix (ECM) that can accommodate cells and regulate their growth leading to three-dimensional tissue regeneration.…”

Section: Pullulanmentioning

confidence: 99%

Natural Products: A Minefield of Biomaterials

Ige

Umoru

Aribo

2012

ISRN Materials Science

157

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The development of natural biomaterials is not regarded as a new area of science, but has existed for centuries. The use of natural products as a biomaterial is currently undergoing a renaissance in the biomedical field. The major limitations of natural biomaterials are due to the immunogenic response that can occur following implantation and the lot-to-lot variability in molecular structure associated with animal sourcing. The chemical stability and biocompatibility of natural products in the body greatly accounts for their utilization in recent times. The paper succinctly defines biomaterials in terms of natural products and also that natural products as materials in biomedical fields are considerably versatile and promising. The various types of natural products and forms of biomaterials are highlighted. Three main areas of applications of natural products as materials in medicine are described, namely, wound management products, drug delivery systems, and tissue engineering. This paper presents a brief history of natural products as biomaterials, various types of natural biomaterials, properties, demand and economic importance, and the area of application of natural biomaterials in recent times.

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Nanogel-quantum dot hybrid nanoparticles for live cell imaging

Cited by 201 publications

References 27 publications

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

Inhibition of the formation of amyloid β‐protein fibrils using biocompatible nanogels as artificial chaperones

Natural Products: A Minefield of Biomaterials

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