Nanogrooved substrate promotes direct lineage reprogramming of fibroblasts to functional induced dopaminergic neurons

Yoo, Jang-Yong; Noh, Myungkyung; Kim, Hongnam; Jeon, Noo Li; Kim, Byung Soo; Kim, Jong-Pil

doi:10.1016/j.biomaterials.2014.12.049

Cited by 71 publications

(76 citation statements)

References 37 publications

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“…On the other hand, substrate topography and mechanical properties may modulate the efficiency of cell reprogramming. Literature demonstrated that cells grown on softer or nanogrooved substrates promoted the reprogramming of somatic cells into neurons [17,18]. Although the above substrates could promote cellular reprogramming, the preparation of these substrates was rather complicated.…”

Section: Discussionmentioning

confidence: 99%

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Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair

et al. 2016

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Section: Discussionmentioning

confidence: 99%

“…Biophysical factors such as mechanical properties and substrate topography also play important roles in cellular reprogramming [17]. Topographical cues may affect the direct reprogramming of fibroblasts into induced neurons [18].…”

Section: Introductionmentioning

confidence: 99%

Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair

et al. 2016

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“…As shown in Table 1, many groups independently identify various transcription factor combinations that can be used to convert fibroblasts to induced dopaminergic (iDA) neurons [8], [9], [15], [16], [31], [32], [33], [34], [35]. This is accomplished by overexpression of transcription factors involved in the specification of dopaminergic neurons during development, together with a combination of small molecule compounds and neurotrophic factors to facilitate the survival and maturation of converted iDA neurons.…”

Section: Direct Conversion Of Fibroblasts To Induced Dopaminergic Neumentioning

confidence: 99%

Induced dopaminergic neurons: A new promise for Parkinson’s disease

Xu¹,

Chu

Jiang

et al. 2017

Redox Biology

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Motor symptoms that define Parkinson’s disease (PD) are caused by the selective loss of nigral dopaminergic (DA) neurons. Cell replacement therapy for PD has been focused on midbrain DA neurons derived from human fetal mesencephalic tissue, human embryonic stem cells (hESC) or human induced pluripotent stem cells (iPSC). Recent development in the direct conversion of human fibroblasts to induced dopaminergic (iDA) neurons offers new opportunities for transplantation study and disease modeling in PD. The iDA neurons are generated directly from human fibroblasts in a short period of time, bypassing lengthy differentiation process from human pluripotent stem cells and the concern for potentially tumorigenic mitotic cells. They exhibit functional dopaminergic neurotransmission and relieve locomotor symptoms in animal models of Parkinson’s disease. In this review, we will discuss this recent development and its implications to Parkinson’s disease research and therapy.

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“…Specifically, the flat, microgrooved, and nanogrooved substates worked differently in terms of cell alignment. One group compared this by manufacturing the patterns using UV-assisted capillary force lithography and analyzed the direct reprogramming of somatic fibroblasts to neurons [70]. These passive topographical cues could offer a simple and effective possibility to enhance the efficiency of cell reprogramming.…”

Section: Introductionmentioning

confidence: 99%

Direct reprogramming and biomaterials for controlling cell fate

Kim

Tae

2016

Biomater Res

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Direct reprogramming which changes the fate of matured cell is a very useful technique with a great interest recently. This approach can eliminate the drawbacks of direct usage of stem cells and allow the patient specific treatment in regenerative medicine. Overexpression of diverse factors such as general reprogramming factors or lineage specific transcription factors can change the fate of already differentiated cells. On the other hand, biomaterials can provide physical and topographical cues or biochemical cues on cells, which can dictate or significantly affect the differentiation of stem cells. The role of biomaterials on direct reprogramming has not been elucidated much, but will be potentially significant to improve the efficiency or specificity of direct reprogramming. In this review, the strategies for general direct reprogramming and biomaterials-guided stem cell differentiation are summarized with the addition of the up-to-date progress on biomaterials for direct reprogramming.

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Nanogrooved substrate promotes direct lineage reprogramming of fibroblasts to functional induced dopaminergic neurons

Cited by 71 publications

References 37 publications

Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair

Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair

Induced dopaminergic neurons: A new promise for Parkinson’s disease

Direct reprogramming and biomaterials for controlling cell fate

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