Nanoinjection of neurotransmitters into the prothoracic ganglion of female cricket <scp><i>Acheta domesticus</i></scp> changes phonotactic selectivity

Atkins, Gordon; Yoon, James; Lee, Kristin; Koo, Rachel; Chung, Kristin; Zdor, John; Magno, Darley; Cho, Eun Byeol; Kim, Cassie; Gonzalez‐Socoloske, Daniel; Navia, Benjamin

doi:10.1111/phen.12330

Cited by 2 publications

(2 citation statements)

References 49 publications

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“…The idea that OA may inhibit central networks involved in phonotactic behavior agrees with the recently reported OA-induced suppression of phonotaxis observed in a different species of cricket, Acheta domesticus [ 17 ]. In that study, OA was injected directly into the prothoracic ganglion, resulting in a reduced phonotactic response.…”

Section: Discussionsupporting

confidence: 89%

“…In particular, chlodimeform (CDM), an octopaminergic agonist that is able to cross the blood–brain barrier in insects, reproduces the activating effect of OA on the release of suppressed aggression in male losers, having the opposite effect on the avoidance behavior [ 12 , 15 ]. At the same time, the inhibitory effect of OA and its agonists on positive phonotaxis was shown in females of the house cricket, Acheta domesticus [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Monoaminergic Systems in Flight-Induced Potentiation of Phonotactic Behavior in Female Crickets Gryllus bimaculatus

Mezheritskiy,

Melnikova,

Dyakonova

et al. 2024

Insects

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We have recently shown that experience of flight remarkably enhanced subsequent terrestrial phonotaxis in females in response to the male calling song. Here, we elucidated the possible roles of octopamine and serotonin in the enhancing effect of flying on phonotactic behavior. Octopamine is known to be released into the hemolymph during flight in insects; however, the octopamine receptor antagonist epinastine did not abolish the effects of flight in our study. On the contrary, the drug significantly potentiated the influence of flying on phonotactic behavior. The octopamine receptor agonist chlordimeform, at a concentration of 2 mM, which was previously found to activate aggression in crickets, dramatically reduced the phonotactic response. However, at a 10-times-lower dose, chlordimeform produced a light but significant decrease in the time that females took to reach the source of the calling song. A similar effect was produced by octopamine itself, which hardly passes the blood–brain barrier in insects. The effect of flight was completely abolished in female crickets treated with alpha-methyl tryptophan (AMTP). AMPT suppresses the synthesis of serotonin, decreasing its content in the nervous systems of insects, including crickets. An activation of the serotonin synthesis with 5-hydroxytryptophan mimicked the effect of flight by increasing the number of visits to and the time spent in the zone near the source of the calling song. The 5-HT content in the third thoracic ganglion was significantly higher in flyers compared to the control group. In contrast, no changes in the octopamine level were observed in the third thoracic ganglion, which is known to play a crucial role in decision-making involved in intraspecific interactions. Therefore, the results suggest that although octopamine is known to be released into the hemolymph during flight, it is likely to inhibit rather than activate the central mechanisms related to phonotaxis. The weak facilitating effect of a low dose of chlordimeform can be attributed to the activation of peripheral octopaminergic receptors. Our results suggest that the serotoninergic system may contribute to the facilitation of female phonotactic behavior by flying. We suggest that both flying and serotonin enhance sexual motivation in females and, by these means, impact their behavioral response to the male calling song.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Monoaminergic Systems in Flight-Induced Potentiation of Phonotactic Behavior in Female Crickets Gryllus bimaculatus

Mezheritskiy,

Melnikova,

Dyakonova

et al. 2024

Insects

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H7 modulation of the L3 auditory neuron and phonotaxis in the cricket Acheta domesticus

Navia,

Widdicombe,

Kim

et al. 2024

Physiological Entomology

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Several studies have implicated the L3 auditory interneuron in the regulation of syllable period selective phonotaxis in female cricket Acheta domesticus. The L3's response to model calls of conspecific males comprises of an immediate and a prolonged response. The kinetics of activation of these electrical activities are consistent with sequential activation of ionotropic and metabotropic mechanisms. In this study, we used electrophysiological and pharmacological tools to investigate the cellular mechanisms underlying L3's response. Bath application of the synthetic protein kinase inhibitor 1‐(5‐isoquinolinesul‐fonyl)‐2‐methylpiperazine (H7), results in the suppression of L3's spiking response, and this effect can be reversed by saline wash. Additionally, when female A. domesticus that were previously nano‐injected with H7 were tested for phonotaxis on a non‐compensating treadmill, they demonstrated suppression of syllable period‐dependent phonotaxis. These findings implicate protein kinase in the regulation of L3's spiking rhythm and the associated phonotaxis in A. domesticus.

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Nanoinjection of neurotransmitters into the prothoracic ganglion of female cricket Acheta domesticus changes phonotactic selectivity

Cited by 2 publications

References 49 publications

Monoaminergic Systems in Flight-Induced Potentiation of Phonotactic Behavior in Female Crickets Gryllus bimaculatus

Monoaminergic Systems in Flight-Induced Potentiation of Phonotactic Behavior in Female Crickets Gryllus bimaculatus

H7 modulation of the L3 auditory neuron and phonotaxis in the cricket Acheta domesticus

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