Nanoinjection system for precise direct delivery of biomolecules into single cells

Yun, Chang-Koo; Hwang, Jung Wook; Kwak, Tae Joon; Chang, Woo-Jin; Ha, Sungjae; Han, Kyutae; Lee, Sang‐Hyun; Choi, Yong‐Soo

doi:10.1039/c8lc00709h

Cited by 14 publications

(12 citation statements)

References 49 publications

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“…Physical methods such as microinjection, 68,69 microfluidic, 70,71 sonication, 72,73 centrifugation, 74 cellular deformation, 75 laser irradiation 76,77 or electroporation 78,79 induce or facilitate cell entry of vectors by mechanical force, mainly through disrupting the plasma membrane. They are commonly applied in non-viral gene delivery because they transport the genetic information easily without the need for carriers and the otherwise low infectivity.…”

Section: Physical Methodsmentioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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Section: Physical Methodsmentioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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“…With the assistance of an electric field to form pores in the membrane, the red fluorescent protein (RFP) was manually injected into the trapped cell with a nanochannel (Figure 9d). [ 124 ] RFP was controllably delivered this way into several different cell types.…”

Section: Micro‐ and Nanoinjectionmentioning

confidence: 99%

Intracellular Labeling with Extrinsic Probes: Delivery Strategies and Applications

Liu

Fraire

Smedt

et al. 2020

Small

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Extrinsic probes have outstanding properties for intracellular labeling to visualize dynamic processes in and of living cells, both in vitro and in vivo. Since extrinsic probes are in many cases cell‐impermeable, different biochemical, and physical approaches have been used to break the cell membrane barrier for direct delivery into the cytoplasm. In this Review, these intracellular delivery strategies are discussed, briefly explaining the mechanisms and how they are used for live‐cell labeling applications. Methods that are discussed include three biochemical agents that are used for this purpose—purpose‐different nanocarriers, cell penetrating peptides and the pore‐foraming bacterial toxin streptolysin O. Most successful intracellular label delivery methods are, however, based on physical principles to permeabilize the membrane and include electroporation, laser‐induced photoporation, micro‐ and nanoinjection, nanoneedles or nanostraws, microfluidics, and nanomachines. The strengths and weaknesses of each strategy are discussed with a systematic comparison provided. Finally, the extrinsic probes that are reported for intracellular labeling so‐far are summarized, together with the delivery strategies that are used and their performance. This combined information should provide for a useful guide for choosing the most suitable delivery method for the desired probes.

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“…A multiple channel design allows parallel single cell electroporation (Khine et al, 2005). Lee, Choi and coworkers reported a nanoinjection system for the delivery of biomolecules into single suspended cells (Yun et al, 2019). The system contained a hybrid (PDMS/glass) microfluidic chip, with microfluidic channel on PDMS and nanoinjection tip on solid glass.…”

Section: Microfluidic Chip For Single-cell Samplingmentioning

confidence: 99%

The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling

Jia

Chen

2020

Front. Chem.

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The research in endogenous biomolecules from a single cell has grown rapidly in recent years since it is critical for dissecting and scrutinizing the complexity of heterogeneous tissues, especially under pathological conditions, and it is also of key importance to understand the biological processes and cellular responses to various perturbations without the limitation of population averaging. Although conventional techniques, such as micromanipulation or cell sorting methods, are already used along with subsequent molecular examinations, it remains a big challenge to develop new approaches to manipulate and directly extract small quantities of cytosol from single living cells. In this sense, nanostructure or nanomaterial may play a critical role in overcoming these challenges in cellular manipulation and extraction of very small quantities of cells, and provide a powerful alternative to conventional techniques. Since the nanostructures or nanomaterial could build channels between intracellular and extracellular components across cell membrane, through which cytosol could be pumped out and transferred to downstream analyses. In this review, we will first brief the traditional methods for single cell analyses, and then shift our focus to some most promising methods for single-cell sampling with nanostructures, such as glass nanopipette, nanostraw, carbon nanotube probes and other nanomaterial. In this context, particular attentions will be paid to their principles, preparations, operations, superiorities and drawbacks, and meanwhile the great potential of nano-materials for single-cell sampling will also be highlighted and prospected.

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Nanoinjection system for precise direct delivery of biomolecules into single cells

Abstract: We report a three-dimensional nanoinjection system for direct injection of biomolecules into a single cell.

Cited by 14 publications

References 49 publications

Materials promoting viral gene delivery

Materials promoting viral gene delivery

Intracellular Labeling with Extrinsic Probes: Delivery Strategies and Applications

The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling

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