Nanomanipulation of single DNA molecules and its applications

Hu, Jun; Zhang, Y.; Li, B.; Gao, H. B.; Hartmann, Uwe; Li, M. Q.

doi:10.1002/sia.1666

Cited by 25 publications

(11 citation statements)

References 17 publications

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“…Examples of AFM applications in imaging and nanomanipulation include the extraction of chromosomal DNA for genetic analysis, the disruption of antibody-antigen bonds, the dissection of biological membranes, and the nano-dissection of protein complexes (Fotiadis et al, 2002). Hu et al (2004) and Lu (2004) investigate the use of an AFM to manipulate single DNA molecules on a substrate. For both works, the DNA samples were initially prepared with a process known as "molecular combing" to remove any random coiling of the DNA.…”

Section: Manipulation Of Biological Materialsmentioning

confidence: 99%

“…Lu (2004) investigates the use of the AFM tip to kink, cut, and displace parts of a DNA strand. Hu et al (2004) demonstrates the ability to spell out the letters "DNA" with the DNA strands through nanomanipulation. In addition to this, folding of the DNA strands was stimulated by pushing near the ends of the DNA with the AFM tip.…”

Section: Manipulation Of Biological Materialsmentioning

confidence: 99%

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A review of research in the field of nanorobotics.

Sierra¹,

Weir²,

Jones³

2005

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This report highlights the findings of an extensive review of the literature in the area of nanorobotics. The main goal of this midyear LDRD effort is to survey and identify accomplishments and advancements that have been made in this relatively new and emerging field. As a result, it may be determined what routes in the area of nanorobotics are scientifically plausible and technically useful so that the Intelligent Systems and Robotics Center can position itself to play a role in the future development of nanotechnology. 4This page intentionally left blank 5

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Section: Manipulation Of Biological Materialsmentioning

confidence: 99%

Section: Manipulation Of Biological Materialsmentioning

confidence: 99%

A review of research in the field of nanorobotics.

Sierra¹,

Weir²,

Jones³

2005

View full text Add to dashboard Cite

show abstract

“…However, with our single molecule method, we isolate and sequence only a single contiguous, comparatively long DNA molecule. As a result, this nanomanipulative method can complement existing sequencing methods by verifying existing assemblies and filling in the ‘gaps’ in the sequenced genome that are particularly recalcitrant with existing technologies, such as the repetitive sequences (Hu et al , 2004; Lü et al , 2004, 2006; An et al ., 2007).…”

Section: Introductionmentioning

confidence: 99%

Optimizing single DNA molecules manipulation by AFM

Long

Lü

Zhang

et al. 2011

Journal of Microscopy

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SummaryRecent progress in single DNA manipulation with atomic force microscope has proved its potential in analysing genetic information at the molecular level instead of macro ensemble approach. However, current manipulation of DNA is mainly carried out by manual operations, which is labour-intensive and time-consuming, thus limits the further applications in other fields. In this paper, an optimized DNA manipulation method is successfully established. An image correlation technique is introduced to realize automatic thermal drift compensation. Combined with elaborately designed tip movement control for different manipulation purposes, accurate and efficient DNA manipulations such as dissection, folding and picking are realized. The efficiency has been improved for an order of magnitude compare to manual manipulations, whereas the performance is demonstrated to be the same. This newly developed method has shed light on high-efficiency nanomanipulation of small molecules with complex structures, and thus provides the possibility of deeply understanding the intrinsic properties of single biomolecules.

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“…As an important application domain, AFM based nanomanipulation made a great progress in recent years. Various AFM based nanomanipulation systems and manipulation schemes have been developed [2][3][4][5][6][7]. In order to facilitate the nanomanipulation, haptic devices and virtual reality interfaces were introduced into the AFM based nanomanipulation systems [8,9], thereby enabling an operator to directly interact with the real nano-world.…”

Section: Introductionmentioning

confidence: 99%

Calibration and nonlinearity compensation for force application in AFM based nanomanipulation

Xie

Vitard

Haliyo

et al. 2008

2008 IEEE/RSJ International Conference on Intelligent Robots and Systems

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Abstract-Both the extent and accuracy of force application in atomic force microscope (AFM) nanomanipulation are significantly limited by the nonlinearity of the commonly used optical lever with a nonlinear position-sensitive detector (PSD). In order to compensate the nonlinearity of the optical lever, a nonlinear calibration method is presented. This method applies the nonlinear curve fit to a full-range position-voltage response of the photodiode, obtaining a continuous function of its voltagerelated sensitivity. Thus, Interaction forces can be defined as integrals of this sensitivity function between any two responses of photodiode voltage outputs, instead of rough transformation with a single conversion factor. The lateral position-voltage response of the photodiode, a universally acknowledged puzzle, was directly characterized by an accurately calibrated force sensor composed of a tippless piezoresistive force sensor, regardless of any knowledge of the cantilevers and laser measuring system. Experiments using a rectangular cantilever (normal force constant 0.24 N/m) demonstrated that the proposed nonlinear calibration method restrained the sensitivity error of normal position-voltage responses to 3.6% and extended the force application range.

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Nanomanipulation of single DNA molecules and its applications

Cited by 25 publications

References 17 publications

A review of research in the field of nanorobotics.

A review of research in the field of nanorobotics.

Optimizing single DNA molecules manipulation by AFM

Calibration and nonlinearity compensation for force application in AFM based nanomanipulation

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