Nanomaterial-based aptamer biosensors for ochratoxin A detection: a review

Sensitive microscale thermophoresis assay for rapid ochratoxin A detection with fluorescently labeled engineered aptamer

“…The detection limit of the aptamer MST assay for OTA is lower than or comparable to some of the reported aptamer-based methods, [40][41][42][43][44] but higher than those of the electrochemical sensors using strand-displacement polymerase reaction, 45 and of some methods using nanomaterials to achieve signal amplification (Table S5 †). 46,47 Compared with other methods, this aptamer MST assay shows many advantages. MST analysis requires only a simple mixing and measuring step without complex and time-consuming procedures of electrode and nanomaterial fabrication.…”

Fluorescent Sensor Based on Magnetic Separation and Strand Displacement Amplification for the Sensitive Detection of Ochratoxin A

“…Therefore, the application of magnetic Fe 3 O 4 nanomaterials can significantly improve the sensitivity and selectivity of detection assays. 41 …”

“…Therefore, the application of magnetic Fe 3 O 4 nanomaterials can significantly improve the sensitivity and selectivity of detection assays. 41 Common Apt-based FL signal amplification strategies mainly include rolling circle amplification (RCA), 42,43 polymerase chain reaction (PCR), 44 hybridization chain reaction(HCR), 28,45,46 strand displacement amplification (SDA), 47 and so forth comparing with RCA, PCR, and HCR, SDA does not require a specific temperature program to react and is an in vitro isothermal amplification technique. The DNA polymerization reaction of SDA involves the continuous replication of one strand of a DNA double-strand using a polymerase.…”

“…In particular, magnetic separation induces interferences from the complex sample matrix. Therefore, the application of magnetic Fe 3 O 4 nanomaterials can significantly improve the sensitivity and selectivity of detection assays …”

Design of a label-free aptasensor for electrochemical determination of hemoglobin: investigation of the peroxidase-like activity of hemoglobin for the sensing of different substrates

“…For this reason, various types of nanomaterials have been used to enhance the sensitivity and selectivity of biosensors. [17][18][19] In this work a hemoglobin-aptamer was immobilized on a screen printed carbon electrode (SPCE) via L-cysteine anchored on AuNPs. For that, gold nanoparticles have been electrodeposited on the SPCE surface, using HAuCl 4 and Na 2 SO 4 .…”