Nanomaterial-based fluorescent biosensors for the detection of antibiotics in foodstuffs: A review

Singh, Harpreet; Thakur, Bandana; Bhardwaj, Sanjeev K.; Khatri, Madhu; Kim, Ki‐Hyun; Bhardwaj, Neha

doi:10.1016/j.foodchem.2023.136657

Cited by 48 publications

(5 citation statements)

References 108 publications

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“…The effectiveness of antibiotic detection methods varies widely, as noted by Singh et al [ 68 ], ranging from microbiological approaches to the use of biosensors with fluorescent nanomaterials. Notably, nanomaterials in biosensors offer a promising alternative to conventional techniques, allowing specific and quantitative detection.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-Resistant Bacteria Isolated from Street Foods: A Systematic Review

Fusaro,

Miranda-Madera,

Serrano-Silva

et al. 2024

Antibiotics

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Street food may be a vehicle of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) to humans. Foods contaminated with ARB entail serious problems or challenges in the fields of medical care, animal husbandry, food industry, and public health worldwide. The objectives of this systematic review were to identify and evaluate scientific reports associated with ARB isolated from various street foods. “Preferred reporting items for systematic reviews and meta-analysis” (PRISMA) guidelines were followed. The bibliographic material covers a period from January 2015 to April 2024. Six electronic scientific databases were searched individually for full-text articles; only those papers that met the inclusion and exclusion criteria were selected. Seventeen papers were included in this systematic review. This study highlighted the wide distribution of ARB resistant to β-lactams and other antibiotics, posing significant health risks to consumers. High resistance levels were observed for antibiotics such as ampicillin, ceftriaxone, and tetracycline, while some antibiotics, such as ceftazidime, clavulanic acid, cefoperazone, cotrimoxazole, doxycycline, doripenem, fosfomycin, vancomycin, and piperacillin-tazobactam, demonstrated 100% susceptibility. The prevalence of ARB in street foods varied between 5.2% and 70.8% among different countries. The multiple resistance of various bacteria, including Escherichia coli, Staphylococcus, Salmonella, and Klebsiella, to multiple classes of antibiotics, as well as environmental factors contributing to the spread of antibiotic resistance (AR), emphasize the urgent need for comprehensive approaches and coordinated efforts to confront antimicrobial resistance (AMR) under the “One Health” paradigm.

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Section: Discussionmentioning

confidence: 99%

Antibiotic-Resistant Bacteria Isolated from Street Foods: A Systematic Review

Fusaro,

Miranda-Madera,

Serrano-Silva

et al. 2024

Antibiotics

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“…Also, regarding the effects of the excessive use of antibiotics, Singh et al report that the direct effects are related to the emergence of resistance in human intestinal flora, associated with the consumption of meat products containing antibiotics. The indirect and long-term effects of the indiscriminate use of antibiotics in humans are carcinogenicity, damage to the reproductive system, and teratogenicity [40].…”

Section: Antibiotics and Electrochemical Detectionmentioning

confidence: 99%

Relevant Aspects in the Development of Electrochemical Aptasensors for the Determination of Antibiotics—A Review

da Silva,

Pereira

2023

Electrochem

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Aptamers are three-dimensional structures of DNA or RNA that present high affinity and selectivity to specific targets, obtained through in vitro screening. Aptamers are used as biological recognizers in electrochemical biosensors, the so-called aptasensors, providing greater specificity in recognizing the most diverse analytes. Electrochemical aptasensors have extremely relevant characteristics, such as high sensitivity, low cost compared to other biorecognizers such as antibodies, and excellent compatibility, being considered one of the most promising alternative methods in several areas, such as biomedical diagnosis and monitoring environmental contaminants. In this sense, the present work reviews the relevant aspects of methodologies based on electrochemical aptasensors and their applications in determining antibiotics, seeking to foster innovation in electrochemical biosensors.

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“…[ 18 ]. Compared with traditional detection methods, biosensors are highly sensitive, highly specific, rapid, cost-effective, easy to use, and portable [ 19 ]. Recently, electrochemical sensors, fluorescent sensors, surface plasma sensors, etc., have been used for the detection of antibiotic residues of animal origin [ 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Based Sensor for Rapid and Sensitive Detection of Ofloxacin in Meat Products

Wang,

Bao,

Liu

et al. 2024

Sensors

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Ofloxacin (OFL) is widely used in animal husbandry and aquaculture due to its low price and broad spectrum of bacterial inhibition, etc. However, it is difficult to degrade and is retained in animal-derived food products, which are hazardous to human health. In this study, a simple and efficient method was developed for the detection of OFL residues in meat products. OFL coupled with amino magnetic beads by an amination reaction was used as a stationary phase. Aptamer AWO-06, which showed high affinity and specificity for OFL, was screened using the exponential enrichment (SELEX) technique. A fluorescent biosensor was developed by using AWO-06 as a probe and graphene oxide (GO) as a quencher. The OFL detection results could be obtained within 6 min. The linear range was observed in the range of 10–300 nM of the OFL concentration, and the limit of the detection of the sensor was 0.61 nM. Furthermore, the biosensor was stored at room temperature for more than 2 months, and its performance did not change. The developed biosensor in this study is easy to operate and rapid in response, and it is suitable for on-site detection. This study provided a novel method for the detection of OFL residues in meat products.

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Nanomaterial-based fluorescent biosensors for the detection of antibiotics in foodstuffs: A review

Cited by 48 publications

References 108 publications

Antibiotic-Resistant Bacteria Isolated from Street Foods: A Systematic Review

Antibiotic-Resistant Bacteria Isolated from Street Foods: A Systematic Review

Relevant Aspects in the Development of Electrochemical Aptasensors for the Determination of Antibiotics—A Review

Aptamer-Based Sensor for Rapid and Sensitive Detection of Ofloxacin in Meat Products

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