Nanomaterial–protein interactions: the case of pristine and functionalized carbon nanotubes and porcine gastric mucin

Barbero, Nadia; Marenchino, Marco; Campos-Olivas, Ramón; Oliaro‐Bosso, Simonetta; Bonandini, Luca; Boskovic, Jasminka; Viscardi, Guido; Visentin, Sonja

doi:10.1007/s11051-016-3388-z

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…Similar results were also reported for pig gastric mucins (PGM). 95,97 Although these studies point to the potential dangers of mucin-mediated cellular uptake of hydrocarbons, they also suggest that mucins could help to efficiently deliver hydrophobic therapeutics to cells. Similarly, the capacity of jellyfish mucus to strongly interact with gold nanoparticles implies that mucus could mediate the toxicity of nanoparticles but also points to the use of mucin-based materials for decontamination purposes.…”

Section: Exploiting the Complex Chemistry Of Mucinsmentioning

confidence: 99%

Mucins as multifunctional building blocks of biomaterials

2018

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Mucins are large glycoproteins that are ubiquitous in the animal kingdom. Mucins coat the surfaces of many cell types and can be secreted to form mucus gels that assume important physiological roles in many animals. Our growing understanding of the structure and function of mucin molecules and their functionalities has sparked interest in investigating the use of mucins as building blocks for innovative functional biomaterials. These pioneering studies have explored how new biomaterials can benefit from the barrier properties, hydration and lubrication properties, unique chemical diversity, and bioactivities of mucins. Owing to their multifunctionality, mucins have been used in a wide variety of applications, including as antifouling coatings, as selective filters, and artificial tears and saliva, as basis for cosmetics, as drug delivery materials, and as natural detergents. In this review, we summarize the current knowledge regarding key mucin properties and survey how they have been put to use. We offer a vision for how mucins could be used in the near future and what challenges await the field before biomaterials made of mucins and mucin-mimics can be translated into commercial products.

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Section: Exploiting the Complex Chemistry Of Mucinsmentioning

confidence: 99%

Mucins as multifunctional building blocks of biomaterials

2018

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“…Inhaled tobramycin associated with aztreonam lysine is usually the first line of treatment for early infections. The affinity of some drugs for mucin has been previously studied using spectroscopic methods by Pontremoli et al (Pontremoli et al, 2015), and by chromatographic methods by Gargano et al (Gargano et al, 2014), whereas Barbero et al have investigated the interactions of both carbon nanotubes (CNTs) and gold nanoparticles (GNPs) with mucin (Barbero et al, 2018(Barbero et al, , 2016). Yet, much needs still to be uncover about mucin-drugs interaction and the physico-chemical parameters that govern the affinity of drugs for mucin.…”

Section: Introductionmentioning

confidence: 99%

Mucin binding to therapeutic molecules: The case of antimicrobial agents used in cystic fibrosis

Butnarasu

Barbero

Pacheco

et al. 2019

International Journal of Pharmaceutics

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Mucin is a complex glycoprotein consisting of a wide variety of functional groups that can interact with exogenous agents. The binding to mucin plays a crucial role in drug pharmacokinetics especially in diseases, such as cystic fibrosis (CF), where mucin is overexpressed. In this study, we have investigated the interaction between mucin and several drugs used in CF therapy. Protein-drug interaction was carried out by UV-Vis and fluorescence spectroscopy; quenching mechanism, binding constants, number of binding sites, thermodynamic parameters and binding distance of the interaction were obtained.

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“…In the human body, GNPs have a good distribution and can easily get to the target cells, where they can carry out their action. Once GNPs enter the body, they are exposed to various molecules and can contact and adsorb many proteins such as ubiquitin, serum albumin, tumor necrosis factor (TNF), fibrinogen (Canoa et al, 2015) and cytochrome C. Many studies have investigated these interactions that can induce structural and physiological changes on the biomolecules (Dobrovolskaia et al, 2009); in particular, ubiquitous proteins are able to interact with GNPs and form a protein corona that influences the distribution of nanoparticles in the human body (Wang et al, 2015) In our previous paper we studied the interaction between CNTs and mucin from porcine stomach Type III (PGM) and it was demonstrated that the superficial functionalization of the material has a great influence on the binding (Barbero et al, 2016). Following these reasons, we studied the interaction between gold nanoparticles and mucin from two commercial mucins, porcine stomach Type III (PGM) and mucin from bovine submaxillary glands (BSM), in order to define their affinity with GNPs.…”

Section: Introductionmentioning

confidence: 99%

Exploring gold nanoparticles interaction with mucins: A spectroscopic-based study

Barbero

Coletti

Catalano

et al. 2018

International Journal of Pharmaceutics

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The interaction between two mucin types (mucin from porcine stomach - PGM and mucin from bovine submaxillary glands - BSM) and gold nanoparticles (GNPs) of various size (5, 20 and 40 nm) and functionalization (with cysteamine or thioglycolic acid) was studied under physiological conditions, in order to investigate the affinity of the nanoparticles to the proteins. Different methods are employed to monitor the interactions: UV-vis and fluorescence spectroscopy, fluorescence lifetime, circular dichroism and transmission electron microscopy. These studies have shown the formation of a complex between GNPs and both PGM and BSM. This aspect could be of great importance for the use of gold nanoparticles for biomedical purposes in those diseases where qualitative and quantitative mucin anomalies play an essential role in mucus composition and rheology.

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Nanomaterial–protein interactions: the case of pristine and functionalized carbon nanotubes and porcine gastric mucin

Cited by 7 publications

References 19 publications

Mucins as multifunctional building blocks of biomaterials

Mucins as multifunctional building blocks of biomaterials

Mucin binding to therapeutic molecules: The case of antimicrobial agents used in cystic fibrosis

Exploring gold nanoparticles interaction with mucins: A spectroscopic-based study

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