Nanomaterials - a New and Former Public Health Issue. The Case of Slovakia

Kuka, Stanislav; Hurbánková, Marta; Drličková, Martina; Baška, Tibor; Hudečková, Henrieta; Tatarková, Zuzana

doi:10.21101/cejph.a4872

Cited by 8 publications

(16 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These sequential molecular and cellular events are known to cause oxidative stress, followed by severe cellular genotoxicity and then programmed cell death. Both experimental and epidemiological studies have indicated that chronic inflammation is involved in and plays a critical role in several chronic diseases, including respiratory and cardiovascular diseases, and lung tumorigenesis [ 17 , 19 , 20 , 21 ]. However, there is no evidence that particles below 100 nm show any step-change in their hazard meaning that there is no evidence of any novel “nano-specific hazard”.…”

Section: Introductionmentioning

confidence: 99%

Markers of Oxidative Stress in the Exhaled Breath Condensate of Workers Handling Nanocomposites

et al. 2018

View full text Add to dashboard Cite

Researchers in nanocomposite processing may inhale a variety of chemical agents, including nanoparticles. This study investigated airway oxidative stress status in the exhaled breath condensate (EBC). Nineteen employees (42.4 ± 11.4 y/o), working in nanocomposites research for 18.0 ± 10.3 years were examined pre-shift and post-shift on a random workday, together with nineteen controls (45.5 ± 11.7 y/o). Panels of oxidative stress biomarkers derived from lipids, nucleic acids, and proteins were analyzed in the EBC. Aerosol exposures were monitored during three major nanoparticle generation operations: smelting and welding (workshop 1) and nanocomposite machining (workshop 2) using a suite of real-time and integrated instruments. Mass concentrations during these operations were 0.120, 1.840, and 0.804 mg/m3, respectively. Median particle number concentrations were 4.8 × 104, 1.3 × 105, and 5.4 × 105 particles/cm3, respectively. Nanoparticles accounted for 95, 40, and 61%, respectively, with prevailing Fe and Mn. All markers of nucleic acid and protein oxidation, malondialdehyde, and aldehydes C6–C13 were elevated, already in the pre-shift samples relative to controls in both workshops. Significant post-shift elevations were documented in lipid oxidation markers. Significant associations were found between working in nanocomposite synthesis and EBC biomarkers. More research is needed to understand the contribution of nanoparticles from nanocomposite processing in inducing oxidative stress, relative to other co-exposures generated during welding, smelting, and secondary oxidation processes, in these workshops.

show abstract

Section: Introductionmentioning

confidence: 99%

Markers of Oxidative Stress in the Exhaled Breath Condensate of Workers Handling Nanocomposites

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Solid carbon-13 NMR data was acquired using a VNMRS 400 widebore spectrometer operating at 399.9 MHz for 1 H and 100.5 MHz for 13 C in a 4 mm Varian Chemagnetics double-resonance probe.…”

Section: Solid-state Carbon-13 Nmrmentioning

confidence: 99%

“…The 1 H spectra (500 MHz, D2O) were acquired using a Varian VNMRS using a switchable broadband probe. 3000 scans were used for the 13 C spectrum and 16 for the proton,…”

Section: Liquid-state Hydrogen and Carbon-13 Nmrmentioning

confidence: 99%

pH-responsive polymer-based biomacromolecule nanosponges as biodegradable carriers for DOX delivery

Nia

Ashkar

Munguia-Lopez

et al. 2021

Preprint

View full text Add to dashboard Cite

Cellulose is the most abundant renewable biomaterial on earth and beta-cyclodextrin (BCD) is among the most commonly used biocompatible drug encapsulation agents. Combining these bio-organic materials is a very powerful approach to greatly enhance the bioavailability of many drugs. These systems also allow for optimal selective drug release profiles, high biocompatibility, as well as “green nanomedicine” approaches that are eco-friendly in their synthesis and have minimal ecological toxicity. herein, we designed a new type of green and biopolymer-based nanosponge drug carriers which is polymerized by crosslinking beta-cyclodextrin ethylene diamine (βCD-EDA) with bifunctional hairy nanocellulose (BHNC). BHNC contains, besides aldehyde groups, carboxyl groups which can react with amino groups in βCD-EDA. Firstly, the crosslinker βCD-EDA was obtained through a simple nucleophilic substitution reaction between beta-cyclodextrin carbonyl imidazole (βCD-CI) and ethylene diamine (EDA). Secondly, BHNC was functionalized with the crosslinker βCD-EDA through a facile nucleophilic substitution crosslinking reaction of the BHNC activated carboxyl groups by the amines of βCD-EDA. We refer to the polymerized highly crosslinked BHNC-βCD-EDA network as BBE. Various ratios of βCD-EDA and BHNC were polymerized with the help of DMTMM as an activator, which resulted in different morphological shapes of BBE, and thus in different release profiles and pH-responsiveness. Unlike other polymer-based βCDs and nanosponges, these new types of crosslinked polymer were prepared in a green and safe solvent (water) and with very short reaction times and at low temperatures. Finally, the BBE polymers were tested as biocompatible nanocarriers for controllable doxorubicin (DOX) delivery. These hyper crosslinked polymers show a high capacity for loading DOX with extended drug release. Furthermore, breast cancer cell cultures show lower cell viability when DOX was loaded in various BBEs than control samples or DOX alone, indicating that our DOX-BBE drug delivery systems are better anticancer agents than DOX alone.

show abstract

“…The European Commission defines nanomaterial as a natural, incidental or manufactured material containing particles in an unbound state or as an aggregate or agglomerate and where, generally for 50% or more of the particles in the number size distribution, one or more external dimensions range from 1-100 nm in size. They can be spherical, tubular, or irregularly shaped and can exist in fused, aggregated or agglomerated forms (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

Comparison of respiratory toxicity of TiO2 and Fe3O4 nanoparticles after intravenous instillation: an experimental study

Hurbánková

Romančíková

Volkovová

et al. 2020

Cent Eur J Public Health

Self Cite

View full text Add to dashboard Cite

Objective: Nanomaterials consist of particles smaller than 100 nm -nanoparticles (NPs). Their nano dimensions allow them to penetrate through various membranes and enter into the bloodstream and disseminate into different body organs. Massive expansion of nanotechnologies together with production of new nanoparticles which have not yet been in contact with living organisms may pose a potential health problem. It is therefore necessary to investigate the health impact of NPs after experimental exposure. Comparison of the effect of TiO 2 and NPs Fe 3 O 4 in Wistar rats at time intervals 1, 7, 14 and 28 days was performed by studying the cytotoxic effect in the isolated inflammatory cells from bronchoalveolar lavage (BAL).Methods: Wistar rats were intravenously (i.v.) given a suspension of NPs TiO 2 or Fe 3 O 4 (coated by sodium oleate) via the tail vein. After time intervals of 1, 7, 14 and 28 days, we sacrificed the animals under anaesthesia, performed BAL and isolated the cells. The number of animals in the individual groups was 7-8. We examined the differential count of BAL cells (alveolar macrophages -AM, polymorphonuclear leukocytes -PMN, lymphocytes -Ly); viability and phagocytic activity of AM; the proportion of immature and polynuclear cells and enzymes -cathepsin D -CAT D, lactate dehydrogenase -LDH and acid phosphatase -ACP.Results: We found that TiO 2 NPs are relatively inert -without induction of inflammatory and cytotoxic response. Exposure to nanoparticles Fe 3 O 4 induced -under the same experimental conditions -in comparison with the control and TiO 2 a more extensive inflammatory and cytotoxic response, albeit only at 1, 7 and 14 days after injection.Conclusions: The results suggest that TiO 2 and Fe 3 O 4 nanoparticles used in our study were transferred from the bloodstream to the respiratory tract, but this effect was not observed at 28 days after i.v. injection, probably due to their removal from the respiratory tract.

show abstract

Nanomaterials - a New and Former Public Health Issue. The Case of Slovakia

Cited by 8 publications

References 14 publications

Markers of Oxidative Stress in the Exhaled Breath Condensate of Workers Handling Nanocomposites

Markers of Oxidative Stress in the Exhaled Breath Condensate of Workers Handling Nanocomposites

pH-responsive polymer-based biomacromolecule nanosponges as biodegradable carriers for DOX delivery

Comparison of respiratory toxicity of TiO<sub>2</sub> and Fe<sub>3</sub>O<sub>4</sub> nanoparticles after intravenous instillation: an experimental study

Contact Info

Product

Resources

About