Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

Lu, Minghua; Yang, Xueqing; Yang, Yixin; Qin, Peige; Wu, Xiuru; Cai, Zongwei

doi:10.3390/nano7040087

Cited by 87 publications

(48 citation statements)

References 124 publications

(132 reference statements)

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“…Because the amount of energy absorbed is related to the type and the number of atoms in the light path, the sample mass concentration can be quantified by comparing the signal with calibration standards at known concentrations. One of the techniques used to determine the chemical composition of a single particle is time-of-flight mass spectrometry (TOFMS) that consists in ionizing small to large organic analytes into the gas phase with minimal fragmentation and their subsequent separation/detection using a time-of-flight mass analyzer [68]. The arrangement of elemental atoms in a nanoparticle may be organized into a crystal structure or it may be amorphous.…”

Section: Chemical Composition and Crystal Structurementioning

confidence: 99%

Polymeric Nanoparticles: Production, Characterization, Toxicology and Ecotoxicology

et al. 2020

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Polymeric nanoparticles (NPs) are particles within the size range from 1 to 1000 nm and can be loaded with active compounds entrapped within or surface-adsorbed onto the polymeric core. The term “nanoparticle” stands for both nanocapsules and nanospheres, which are distinguished by the morphological structure. Polymeric NPs have shown great potential for targeted delivery of drugs for the treatment of several diseases. In this review, we discuss the most commonly used methods for the production and characterization of polymeric NPs, the association efficiency of the active compound to the polymeric core, and the in vitro release mechanisms. As the safety of nanoparticles is a high priority, we also discuss the toxicology and ecotoxicology of nanoparticles to humans and to the environment.

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Section: Chemical Composition and Crystal Structurementioning

confidence: 99%

Polymeric Nanoparticles: Production, Characterization, Toxicology and Ecotoxicology

et al. 2020

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“…Since then, a large number of different materials have been reported as SALDI substrates with varying degrees of performance. 10,11 Back in 1988, Tanaka et al 9 have already defined characteristics of the material suitable to assist LDI, such as strong absorption in the UV range, low heat capacity and large surface area per volume unit. Hence, metal-based materials, comprising chemically stable metal oxides showed promising performance for SALDI-MS. 12 Particularly, TiO 2 powder characterized by its strong UV absorption, mixed with a liquid medium showed the lowest background noise and the highest intensity during the analysis of low-molecular-weight polar and non-polar analytes.…”

Section: Introductionmentioning

confidence: 99%

Functionalized Titanium Oxide Nanowire Substrate for Surface-Assisted Laser Desorption/ionization Imaging Mass Spectrometry

Ep¹,

H²,

Hsu³

et al. 2019

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Imaging mass spectrometry (IMS) is a powerful technique that enables analysis of various molecular species at a high spatial resolution with low detection limits. In contrast to the standard matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) approach, surface-assisted laser desorption/ionization (SALDI) is more effective in the detection of small molecules due to the absence of interfering background signals in low m/z ranges. We developed a functionalized TiO2 nanowire as a solid substrate for IMS of low-molecular-weight species in biological specimens. We prepared TiO2 nanowires using the inexpensive modified hydrothermal process and subsequently functionalized it chemically with various silane analogs to overcome the problem of superhydrophilicity of the substrate. Chemical modification changed the selectivity of imprinting of samples deposited on the surface of the plate and thus improved the detection limits. Due to the enhanced performance, the functionalized TiO2 nanowire substrate could be successfully used for imaging of complex native samples. We applied our new substrate to image distribution of the secondary metabolites in (1) petal of the medicinal plant Catharanthus roseus and (2) microbial co-culture of Burkholderia cenocepacia 869T2 vs Phellinus noxius. We observed that secondary metabolites are distributed heterogeneously in a petal, which is consistent with previous results reported for the C. roseus plant leaf and stem. We verified the semi-quantitative capabilities of the imprinting/imaging approach by comparing results using standard LC-MS analysis of the plant extracts. Several bacteria-related metabolites produced by B. cenocepacia 869T2 in presence of P. noxius, which were unable to be detected by MALDI-MS approach, were revealed by our newly developed approach. This suggested that the functionalized TiO2 nanowire substrates-based SALDI is a powerful technique complementary to MALDI-MS.

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“…The recent evolution of NPs as MALDI matrices has significantly contributed to small molecule analysis by MALDI-MS [10]. Most NPs have UV absorption and can be homogeneously applied, which is useful for imaging applications and to minimize spot-to-spot variation.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle microarray for high-throughput microbiome metabolomics using matrix-assisted laser desorption ionization mass spectrometry

et al. 2018

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A high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI)-MS-based metabolomics platform was developed using a prefabricated microarray of nanoparticles and organic matrices. Selected organic matrices, inorganic nanoparticle (NP) suspensions, and sputter coated metal NPs, as well as various additives, were tested for metabolomics analysis of the turkey gut microbiome. Four NPs and one organic matrix were selected as the optimal matrix set: α-cyano-4-hydroycinnamic acid, Fe3O4 and Au NPs in positive ion mode with 10 mM sodium acetate, and Cu and Ag NPs in negative ion mode with no additive. Using this set of five matrices, over two thousand unique metabolite features were reproducibly detected across intestinal samples from turkeys fed a diet amended with therapeutic or sub-therapeutic antibiotics (200 g/ton or 50 g/ton bacitracin methylene disalicylate (BMD), respectively), or non-amended feed. Among the thousands of unique features, 56 of them were chemically identified using MALDI-MS/MS, with the help of in-parallel liquid chromatography (LC)-MS/MS analysis. Lastly, as a proof of concept application, this protocol was applied to 52 turkey cecal samples at three different time points from the antibiotic feed trial. Statistical analysis indicated variations in the metabolome of turkeys with different ages or treatments.

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Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

Cited by 87 publications

References 124 publications

Polymeric Nanoparticles: Production, Characterization, Toxicology and Ecotoxicology

Polymeric Nanoparticles: Production, Characterization, Toxicology and Ecotoxicology

Functionalized Titanium Oxide Nanowire Substrate for Surface-Assisted Laser Desorption/ionization Imaging Mass Spectrometry

Nanoparticle microarray for high-throughput microbiome metabolomics using matrix-assisted laser desorption ionization mass spectrometry

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