Nanomaterials driven CRISPR/Cas-based biosensing strategies

Huang, Ying; Wen, Qian; Xiong, Ying; Chen, Yanni; Li, Wang; Ren, Jiali; Zhong, Haiyan

doi:10.1016/j.cej.2023.145615

Cited by 4 publications

(3 citation statements)

References 82 publications

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“…Importantly, in practical applications, nanomaterials often fulfill multiple roles through integration rather than functioning in isolation, highlighting their versatility and adaptability in biosensing applications. 56 The use of nanostructured materials in biosensing technologies with transducers for signal amplification in immunosensors has also resulted in a number of appealing platforms. Over time, significant advances in immunosensors have been reported, including a larger surface area, functional interface for biomolecule immobilization, adsorption capabilities, electron-transfer kinetics, and spatial arrangement.…”

Section: Diagnosticsmentioning

confidence: 99%

“…Additionally, they can act as catalysts, exhibiting peroxidase or oxidase properties, as conductors, facilitating electron transfer on electrode surfaces through conductive nanomaterials, and as reporters, possessing fluorescent, Raman, or electrochemical properties. Importantly, in practical applications, nanomaterials often fulfill multiple roles through integration rather than functioning in isolation, highlighting their versatility and adaptability in biosensing applications …”

Section: Non-nucleic Acid (Nna) Target Detectionmentioning

confidence: 99%

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Exploiting the Specificity of CRISPR/Cas System for Nucleic Acids Amplification-Free Disease Diagnostics in the Point-of-Care

Yee,

Ali,

Mohd-Naim

et al. 2024

Chem Bio Eng.

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Rapid and reliable molecular diagnostics employing target nucleic acids and small biomarkers are crucial strategies required for the precise detection of numerous diseases. Although diagnoses based on nucleic acid recognition are some of the most efficient and precise procedures, these tests often require expensive equipment and skilled professionals. Recent advancements in diagnostic innovations, particularly those based on clustered regularly interspaced short palindromic repeats (CRISPR), aim to provide thorough screening at homes, in clinics, and in the field. In comparison to traditional molecular techniques like PCR, CRISPR/Cas-based detection, using the single-stranded nucleic acid trans-cleavage abilities of Cas12 or Cas13, shows significant potential as a molecular diagnostic tool. It offers benefits such as attomolar-level sensitivity, single-base precision, and rapid turnover rates. Both Cas enzymes demonstrate exceptional specificity and sensitivity, holding substantial promise in disease diagnostics and beyond. Consequently, various amplification-free CRISPR/Cas-based detection methods have emerged, aiming to maintain sensitivity despite the absence of pre-amplification. This allows for the detection of non-nucleic acid targets and facilitates integration into point-of-care settings. This Review highlights current advances in amplification-free CRISPR/Cas detection systems in disease diagnostics and investigates their utility in point-of-care settings. Furthermore, the mechanisms of alternative CRISPR-based amplification-free detection of other small molecules, aside from nucleic acids, for disease diagnosis will also be briefly discussed.

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Section: Diagnosticsmentioning

confidence: 99%

Section: Non-nucleic Acid (Nna) Target Detectionmentioning

confidence: 99%

Exploiting the Specificity of CRISPR/Cas System for Nucleic Acids Amplification-Free Disease Diagnostics in the Point-of-Care

Yee,

Ali,

Mohd-Naim

et al. 2024

Chem Bio Eng.

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“…Faced with the oil crisis in the 1970s and growing environmental concerns, research has explored the partial or total replacement of phenolic adhesives with bioadhesives that exhibit comparable or superior physical-mechanical characteristics to conventional ones (Kumar andLeggate 2022, Gu et al 2019). Several studies support the feasibility of reducing the use of synthetic adhesives through the incorporation of nanoscale reinforcing structures (Zidanes et al 2023, Nicolao et al 2022, Huang et al 2023, Shirmohammadli et al 2018.…”

Section: Introductionmentioning

confidence: 98%

Utilization of nanotalc modified adhesives in plywood panels

Lourenço,

Aparecida dos Santos,

Corrêa Furtini

et al. 2024

Maderas-Cienc Tecnol

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As an alternative for the partial replacement of synthetic adhesives are the modifications that occur still during their synthesis, to improve and adhere new properties. Among the possible materials used in nanoscale, talc is a mineral material of natural origin and a promising raw material due to its low cost, lightness, natural hydrophobicity. Due to the scarcity of studies with the insertion of nanotalc in adhesives, this study aimed to produce plywood panels bonded with phenol-formaldehyde adhesive nanomodified with talc to evaluate its physical-mechanical resistance when compared to conventional synthetic adhesives. To carry out this study, three Pinus oocarpa trees with 28 years of age were used. Different concentrations of talc were used in the formulation with the phenol-formaldehyde adhesive, being the treatments 0; 0,05; 1; 1,5 and 2 % of talc (mass/mass) in relation to the adhesive, with three panels per treatment, totaling 15 panels. The pressing time was 8 minutes with temperature of 160 ºC and pressure of 1 MPa. The physical-chemical characteristics of the lignocellulosic material and of the plywood panels were determined. The quality of the adhesive produced was demonstrated by its resistance to water absorption with the insertion of up to 2 % talc to the adhesive, reducing it considerably. From 1 % talc inserted, the plywood panels had their mechanical characteristics superior to those glued with pure phenol-formaldehyde adhesive. However, as the study is innovative in nature, further research should focus on the application and quality evaluation of other talc nanomodified adhesives on different types of wood panels.

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