Nanomechanical mapping of a high curvature polymer brush grafted from a rigid nanoparticle

Dunér, Gunnar; Thormann, Esben; Dédinaité, Andra; Claesson, Per M.; Matyjaszewski, Krzysztof; Tilton, Robert D.

doi:10.1039/c2sm26086g

Cited by 32 publications

(31 citation statements)

References 34 publications

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“…This set-up enables simultaneous mapping of both topography and sample nanomechanical properties in liquid. [31][32][33] In the PeakForce ® measurement, the scanner oscillates at a low frequency (1-3 kHz), resulting in a tip-sample interaction with every cycle. The maximum force on the tip is predetermined and maintained by a feed-back loop during the scan, which provides the opportunity for non-destructive imaging.…”

Section: Microgel Synthesismentioning

confidence: 99%

Factors Affecting Peptide Interactions with Surface-Bound Microgels

et al. 2016

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Effects of electrostatics and peptide size on peptide interactions with surfacebound microgels were investigated with ellipsometry, confocal microscopy, and atomic force microscopy (AFM). Results show that binding of cationic poly-L-lysine (pLys) to anionic, covalently immobilized, poly(ethyl acrylate-co-methacrylic acid) microgels increased with increasing peptide net charge and microgel charge density. Furthermore, peptide release was facilitated by decreasing either microgel or peptide charge density. Analogously, increasing ionic strength facilitated peptide release for short peptides. As a result of peptide binding, the surface-bound microgels displayed pronounced deswelling and increased mechanical rigidity, the latter quantified by quantitative nanomechanical mapping. While short pLys was found to penetrate the entire microgel network and to result in almost complete charge neutralization, larger peptides were partially excluded from the microgel network, forming an outer peptide layer on the microgels. As a result of this difference, microgel flattening was more influenced by the lower Mw peptide than the higher. Peptide-induced deswelling was found to be lower for higher Mw pLys, the latter effect not observed for the corresponding microgels in the dispersed state. While the effects of electrostatics on peptide loading and release were similar to those observed for dispersed microgels, there were thus considerable effects of the underlying surface on peptide-induced microgel deswelling, which need to be considered in the design of surface-bound microgels as carriers of peptide loads, e.g., in drug delivery or in functionalized biomaterials.

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Section: Microgel Synthesismentioning

confidence: 99%

Factors Affecting Peptide Interactions with Surface-Bound Microgels

et al. 2016

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“…[89] AFM analysis AFM is complementary to TEM, as it yields the topography of the sample. [90] It is preferable to SEM in this case, because the resolving power is higher and the samples can be measured without pretreatment. [91] Since it was impossible to use the TEM specimens for the AFM measurements as well, new specimens were prepared by spin-coating the nanohybrids on glass disks from solution in methanol.…”

Section: Grafting Of Polymers With Single Trithiocarbonate Groups To mentioning

confidence: 99%

Nanohybrids of Gold Particles

Ebeling

2015

Springer Theses

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The design and the characterization of the substrate components for this chapter, gold nanocrystals from the ex-situ two-phase Brust-Schiffrin synthesis and from reduction with citrate ions ci Au ν-3 , were described in Section 5.1. In the following, it will be outlined how these AuNPs can be functionalized with non-polymeric ligands of different types for stabilization and introduction of functional groups, especially hydroxyl and azido moieties, to which RAFT agents with complementary functionalities could then be coupled, and how they can be coated with the NIPAm RAFT polymers prepared in Section 5.4. Hereby, the binding motifs of ligands bearing trithiocarbonate groups will be the particular focus. Different ligands on AuNPs Functionalization of Brust-Schiffrin gold nanocrystalsAuNPs prepared by the ex-situ two-phase Brust-Schiffrin method were presented and their ligand-independent properties were characterized in Section 5.1.3.3 (also see Table 5.2, Section 5.1.5). These nanocrystals are very well suited for functionalization reactions for three major reasons:• The weakly bound tetraoctylammonium bromide is readily replaced by all ligands that form stronger bonds to the gold surface.• The sodium borohydride can be completely washed off prior to the addition of the ligand, so that even very reduction-sensitive molecules can be used.• The arising particle size and shape characteristics are independent of the employed ligand, enabling comparative experiments with different functionalization agents.

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“…In PeakForce Ò , the sample surface is moved towards the tip, in contrast to conventional tapping mode AFM. This technique is explained in detail in other publications [13,14,20,21]. Briefly, the surface is moved in a sinusoidal manner with the amplitude of approximately 150 nm at a frequency in the range 1-2 kHz.…”

Section: In-situ Imagingmentioning

confidence: 99%

Role of microstructure on corrosion initiation of an experimental tool alloy: A Quantitative Nanomechanical Property Mapping study

et al. 2014

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a b s t r a c tThe adhesion properties of a FeCrVN experimental tool alloy immersed in pure water and sodium chloride solution have been studied by Quantitative Nanomechanical Property Mapping to understand the influence of microstructure on corrosion initiation of this alloy. The approach used here allows early observation and identification of pre-pitting events that may lead to passivity breakdown of the alloy. Adhesion provides a good distinction between the different regions and we ascribe this to their vanadium and nitrogen contents. Finally, the prepitting is characterized by generation of small particles in specific regions of the surface with low chromium content.

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Nanomechanical mapping of a high curvature polymer brush grafted from a rigid nanoparticle

Cited by 32 publications

References 34 publications

Factors Affecting Peptide Interactions with Surface-Bound Microgels

Factors Affecting Peptide Interactions with Surface-Bound Microgels

Nanohybrids of Gold Particles

Role of microstructure on corrosion initiation of an experimental tool alloy: A Quantitative Nanomechanical Property Mapping study

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