Nanomechanical vibration profiling of oocytes

Peng, Yongpei; Zhang, Junhui; Xue, Weiwei; Wu, Wenjie; Wang, Yu; Mei, Kainan; Chen, Ye; Rao, Depeng; Yan, Tianhao; Wang, Qianqian; Cao, Yunxia; Wu, Shangquan; Zhang, Qingchuan

doi:10.1007/s12274-022-4439-7

Cited by 8 publications

(7 citation statements)

References 39 publications

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“…It has been shown that nanovibrations detected by microcantilevers were related to cell viability in some studies. − We first treated the cells with TGF-β1 for 24, 48, and 72 h and then performed cell viability assays on the cells (Figure S23). The results showed that TGF-β1 treatment did not bring about changes in cell viability.…”

Section: Results and Discussionmentioning

confidence: 99%

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Deep-Learning-Based Nanomechanical Vibration for Rapid and Label-Free Assay of Epithelial Mesenchymal Transition

Wu,

Peng,

et al. 2024

ACS Nano

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Cancer is a profound danger to our life and health. The classification and related studies of epithelial and mesenchymal phenotypes of cancer cells are key scientific questions in cancer research. Here, we investigated cancer cell colonies from a mechanical perspective and developed an assay for classifying epithelial/mesenchymal cancer cell colonies using the biomechanical fingerprint in the form of "nanovibration" in combination with deep learning. The classification method requires only 1 s of vibration data and has a classification accuracy of nearly 92.5%. The method has also been validated for the screening of anticancer drugs. Compared with traditional methods, the method has the advantages of being nondestructive, label-free, and highly sensitive. Furthermore, we proposed a perspective that subcellular structure influences the amplitude and spectrum of nanovibrations and demonstrated it using experiments and numerical simulation. These findings allow internal changes in the cell colony to be manifested by nanovibrations. This work provides a perspective and an ancillary method for cancer cell phenotype diagnosis and promotes the study of biomechanical mechanisms of cancer progression.

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Section: Results and Discussionmentioning

confidence: 99%

“…In recent years, microcantilever has been used to characterize the viability of cells, bacteria, etc. by measuring their active motility. − However, cell viability may not be the only factor affecting nanovibration. Cell colonies consist of multiple single cells, each of which may generate nanovibrations.…”

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confidence: 99%

Deep-Learning-Based Nanomechanical Vibration for Rapid and Label-Free Assay of Epithelial Mesenchymal Transition

Wu,

Peng,

et al. 2024

ACS Nano

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“…AFM can examine samples of different stiffness, regardless of their electrical conductivity, in different environments, from liquid to ultra-high vacuum. This wide range of operating conditions offers the possibility of measuring an equally wide range of samples such as cells [17][18][19], bacteria [20][21][22][23], extracellular vesicles [24,25], self-assembled molecular films [26][27][28][29][30][31][32][33][34][35][36][37][38], Langmuir-Blodgett films [39][40][41][42], supported lipid bilayers (SLB) [43][44][45][46][47][48][49], polymers [50][51][52][53], oxides [54][55][56][57], 2D materials [58][59][60][61][62], and other various materials [63]…”

Section: Introduction On Atomic Force Microscopementioning

confidence: 99%

“…Due to these characteristics, AFM is also well-suited to the study of biomaterials and hydrated biological specimens, as scanning can be carried out in a fluid environment, and specimens require no freezing, fixing, or staining and suffer no dehydration [18,[79][80][81]. The AFM imaging capability is certainly useful, but its overall value is primarily related to the low perturbative nature of the probe/sample interaction, providing a unique insight into the heterogeneous nature of soft samples [82].…”

Section: Introduction On Atomic Force Microscopementioning

confidence: 99%

Morphological Investigation of Protein Crystals by Atomic Force Microscopy

et al. 2023

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In this review, we discuss the progress in the investigation of macromolecular crystals obtained through the use of atomic force microscopy (AFM), a powerful tool for imaging surfaces and specimens at high resolution. AFM enables the visualization of soft samples at the nanoscale and can provide precise visual details over a wide size range, from the molecular level up to hundreds of micrometers. The nonperturbative nature, the ability to scan in a liquid environment, and the lack of need for freezing, fixing, or staining make AFM a well-suited tool for studying fragile samples such as macromolecular crystals. Starting from the first morphological investigations revealing the surface morphology of protein crystals, this review discusses the achievements of AFM in understanding the crystal growth processes, both at the micro- and nanoscale. The capability of AFM to investigate the sample structure at the single molecular level is analyzed considering in-depth the structure of S-layers. Lastly, high-speed atomic force microscopy (HS-AFM) is discussed as the evolution to overcome the limitations of low imaging speed, allowing for the observation of molecular dynamics and weakly adsorbed, diffusing molecules. HS-AFM has provided intuitive views and directly visualized phenomena that were previously described indirectly, answering questions that were challenging to address using other characterization methods.

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“…[24] It has rapidly become a current research hotspot in the field of sensors with very good application prospects. [25][26][27][28][29][30][31] The microcantilever detection technology is based on the optical lever method to read out the displacement of the laser on the position-sensitive detector, which has 10 3 orders of magnitude amplification as compared to the real deflection of the microcantilever tip. In addition, due to its relatively soft nature, the detection sensitivity will be further improved.…”

Section: Introductionmentioning

confidence: 99%

Magneto‐Nanomechanical Array Biosensor for Ultrasensitive Detection of Oncogenic Exosomes for Early Diagnosis of Cancers

Mei

Yan

Wang

et al. 2022

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The Advisory Committee on Cancer for the World Health Organization suggests that "a third of cancers are curable if diagnosed early". [3] Traditional methods of cancer diagnosis are based primarily on histo-pathological examination and imaging of the tumor tissue, which are the current gold standards. [4] However, these methods tend to detect specific lesions only when they are formed in the middle and later stages of cancer development, and are not suitable for screening early cancers in a large population. In the early stage of cancer development, before the formation of specific lesions, cancer cells in the body begin to release a few cancer markers related to cancer formation and metastasis. [5] At this time, if a highly sensitive detection method can be available to detect the very low concentration of markers in body fluids, it will bring great potential for the early detection and diagnosis of cancer. In addition, traditional methods are invasive, and inaccurate biopsy positioning can cause sampling errors. X-ray screening and histo-pathological evaluation rely heavily on the individual's expertise, which causes discrepancies between individual and laboratory-reported results. [6] All these limitations indicate the need for highly sensitive and minimally invasive techniques for early diagnosis and treatment of cancers. In comparison, liquid biopsies can offer early discovery and a non-invasive approach.In recent years, cancer markers in body fluids, such as circulating tumor-derived proteins (carcinoembryonic antigen, prostate-specific antigen), circulating tumor cells, and circulating tumor DNA, [7] have been the most common biomarkers for cancer diagnosis and assessment of treatment efficacy. However, these biomarkers have several shortcomings in early-stage cancer diagnosis. Circulating tumor-derived protein markers are compromised by high false positive rates, which can lead to overdiagnosis and, in some cases, to unnecessary anticancer treatment. [8] The fraction of circulating tumor DNA in total circulating free DNA is usually very low and often < 0.01%; therefore, this can complicate early detection. [9] Circulating tumor cells are tumor cells that have detached from the primary tumor, with extremely low quantities. Exosomes are a class of nanoscale vesicles secreted by cells, which contain abundant information closely related to parental cells. The ultrasensitive detection of cancer-derived exosomes is highly significant for early noninvasive diagnosis of cancer.Here, an ultrasensitive nanomechanical sensor is reported, which uses a magnetic-driven microcantilever array to selectively detect oncogenic exosomes. A magnetic force, which can produce a far greater deflection of microcantilever than that produced by the intermolecular interaction force even with very low concentrations of target substances, is introduced. This method reduced the detection limit to less than 10 exosomes mL −1 . Direct detection of exosomes in the serum of patients with breast cancer and in healthy people showed a signifi...

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Nanomechanical vibration profiling of oocytes

Cited by 8 publications

References 39 publications

Deep-Learning-Based Nanomechanical Vibration for Rapid and Label-Free Assay of Epithelial Mesenchymal Transition

Deep-Learning-Based Nanomechanical Vibration for Rapid and Label-Free Assay of Epithelial Mesenchymal Transition

Morphological Investigation of Protein Crystals by Atomic Force Microscopy

Magneto‐Nanomechanical Array Biosensor for Ultrasensitive Detection of Oncogenic Exosomes for Early Diagnosis of Cancers

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