Nanomechanics of Blood Clot and Thrombus Formation

Domingues, Marco M.; Carvalho, Filomena A.; Santos, Nuno C.

doi:10.1146/annurev-biophys-111821-072110

Cited by 8 publications

(6 citation statements)

References 148 publications

(178 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relationship between thrombotic disorders and clot mechanical properties has been consistently demonstrated. 3,6,[34][35][36][37] For example, studies have reported lower stiffness of in vitro clots of patients with bleeding conditions, whereas higher stiffness was observed in clots made from plasma of patients in thrombotic states. 3,36 Furthermore, plasma clots express unexpectedly low toughness for a ductile material 38 and are less resilient to rupture than other biological hydrogels, such as collagen, cartilage, and others, despite being extremely deformable.…”

Section: Discussionmentioning

confidence: 99%

Mechanics and microstructure of blood plasma clots in shear driven rupture

Ramanujam,

Garyfallogiannis,

Litvinov

et al. 2024

Soft Matter

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Mechanics and microstructure of blood plasma clots in shear driven rupture

Ramanujam,

Garyfallogiannis,

Litvinov

et al. 2024

Soft Matter

View full text Add to dashboard Cite

show abstract

“…This result was credible since good stability and resistance to enzymatic cleavage and shear forces have been demonstrated for fully coagulated BCC. [21][22][23] Therefore, centrifugation performed after BCC formation resulted in a slight impact on leukocyte distribution. The lowest DNA yield was 12.30 μg/g BCC in one upper segment with a leukocyte concentration of 5.27 × 10 6 cells/mL, meaning that adequate DNA for further application could be obtained even with 0.25 g BCC.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of DNA extracted from residual blood clots after serological testing

Zhang,

Cheng,

Yang

et al. 2024

Cell Biochemistry & Function

View full text Add to dashboard Cite

DNA quality is of paramount importance for molecular biology research. This study aimed to assess the DNA extracted from residual blood clots after serological testing, focusing on the impact of blood clot segments, extraction kits, temporary storage durations (TSDs), and thawing methods on DNA quality. We divided the residual blood clot column (BCC) from healthy donors into three segments and utilized two different extraction kits. The BCCs were subjected to four TSDs at 4°C (7 days, 10 days, 1 month, and 2 months) and three thawing methods (4°C, room temperature, and 37°C). We found that the TIANamp Blood Clot DNA Kit yielded consistently high‐quality DNA from each segment with stable A260/280 and A260/230 ratios. The DNA yield showed a strong positive correlation with leukocyte concentration, and a satisfactory median DNA yield of 28.79 μg/g BCC was obtained across all segments. DNA integrity, as measured by the DNA integrity number and DNA fragment peak size, decreased with increasing TSD at 4°C, with a notable decrease after 10 days of storage. Thawing at 37°C resulted in the lowest DNA fragment peak size. In conclusion, BCC could be an ideal DNA source with satisfactory yield and purity. A prolonged TSD at 4°C leads to an obvious decrease in DNA integrity, and thawing the frozen BCC at 37°C decreases DNA fragment sizes. To maintain DNA integrity, BCCs should be cryopreserved as soon as possible after short TSDs at 4°C and thawed at 4°C.

show abstract

“…Further studies should be performed to dissect the role of autophagy in hemostasis-osteogenesis interplay, a recently identified vital part of bone regeneration. It is noteworthy that research on blood clots has gradually emerged in the field of bone regeneration, especially in terms of the kinetics of the contribution of fibrin, tissue factor, and platelets [ 176 ]. Consequently, future studies may need to focus on the relationship between autophagy and the structure/function of clots, whether an interaction between autophagy and the progress of inflammation or the function of cells or platelets, which affect the differences in the structure of thrombi and kinetics of formation as a consequence of bone injury.…”

Section: Therapeutic Approaches For Bone Diseases By Modulating Autop...mentioning

confidence: 99%

The role of autophagy in bone metabolism and clinical significance

et al. 2023

View full text Add to dashboard Cite

The skeletal system is the basis of the vertebral body composition, which affords stabilization sites for muscle attachment, protects vital organs, stores mineral ions, supplies places to the hematopoietic system, and participates in complex endocrine and immune system. Not surprisingly, bones are constantly reabsorbed, formed, and remodeled under physiological conditions. Once bone metabolic homeostasis is interrupted (including inflammation, tumors, fractures, and bone metabolic diseases), the body rapidly initiates bone regeneration to maintain bone tissue structure and quality. Macroautophagy/autophagy is an essential metabolic process in eukaryotic cells, which maintains metabolic energy homeostasis and plays a vital role in bone regeneration by controlling molecular degradation and organelle renewal. One relatively new observation is that mesenchymal cells, osteoblasts, osteoclasts, osteocytes, chondrocytes, and vascularization process exhibit autophagy, and the molecular mechanisms and targets involved are being explored and updated. The role of autophagy is also emerging in degenerative diseases (intervertebral disc degeneration [IVDD], osteoarthritis [OA], etc.) and bone metabolic diseases (osteoporosis [OP], osteitis deformans, osteosclerosis). The use of autophagy regulators to modulate autophagy has benefited bone regeneration, including MTOR (mechanistic target of rapamycin kinase) inhibitors, AMPK activators, and emerging phytochemicals. The application of biomaterials (especially nanomaterials) to trigger autophagy is also an attractive research direction, which can exert superior therapeutic properties from the material-loaded molecules/drugs or the material’s properties such as shape, roughness, surface chemistry, etc. All of these have essential clinical significance with the discovery of autophagy associated signals, pathways, mechanisms, and treatments in bone diseases in the future. Abbreviations: Δψm: mitochondrial transmembrane potential AMPK: AMP-activated protein kinase ARO: autosomal recessive osteosclerosis ATF4: activating transcription factor 4 ATG: autophagy-related β-ECD: β-ecdysone BMSC: bone marrow mesenchymal stem cell ER: endoplasmic reticulum FOXO: forkhead box O GC: glucocorticoid HIF1A/HIF-1α: hypoxia inducible factor 1 subunit alpha HSC: hematopoietic stem cell HSP: heat shock protein IGF1: insulin like growth factor 1 IL1B/IL-1β: interleukin 1 beta IVDD: intervertebral disc degradation LPS: lipopolysaccharide MAPK: mitogen-activated protein kinase MSC: mesenchymal stem cell MTOR: mechanistic target of rapamycin kinase NP: nucleus pulposus NPWT: negative pressure wound therapy OA: osteoarthritis OP: osteoporosis PTH: parathyroid hormone ROS: reactive oxygen species SIRT1: sirtuin 1 SIRT3: sirtuin 3 SQSTM1/p62: sequestosome 1 TNFRSF11B/OPG: TNF receptor superfamily member 11b TNFRSF11A/RANK: tumor necrosis factor receptor superfamily, member 11a TNFSF11/RANKL: tumor necrosis factor (ligand) superfamily, member 11 TSC1: tuberou...

show abstract

Nanomechanics of Blood Clot and Thrombus Formation

Cited by 8 publications

References 148 publications

Mechanics and microstructure of blood plasma clots in shear driven rupture

Mechanics and microstructure of blood plasma clots in shear driven rupture

Evaluation of DNA extracted from residual blood clots after serological testing

The role of autophagy in bone metabolism and clinical significance

Contact Info

Product

Resources

About