In situ transmission electron microscopy was used to observe the electromigration-driven sliding behavior of twinned face-centered cubic-Co fillers sliding inside multi-walled carbon nanotubes with an inner-diameter step. Each filler contained a twin boundary located at the nanotube inner-diameter step. Under application of an electric current, the twinned Co fillers moved across the steps and changed their diameter, and the position of the twin boundary was maintained at the nanotube inner-diameter step.