Nanomonitor: A Miniature Electronic Biosensor for Glycan Biomarker Detection

Nagaraj, Vinay J.; Aithal, Srivatsa; Eaton, Seron; Bothara, Manish; Wiktor, Peter; Prasad, Shalini

doi:10.2217/nnm.10.11

Cited by 63 publications

(68 citation statements)

References 31 publications

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“…[25][26][27]30 In contrast, a linear increase from baseline impedance was observed when the SNA lectin biosensor was incubated with an increasing concentration of fetuin expressing a correct glycan epitope. [25][26][27]30 Apart from showing very high binding specicity towards the glycan epitope, these SNA lectin biosensors have demonstrated sensitivity for glycoprotein detection (e.g. fetuin) up to attomolar concentration.…”

Section: Glycoprotein Capture and Detectionmentioning

confidence: 93%

“…29 In fact, glycan structure specic interactions of a glycoprotein with lectins are very well demonstrated in the recent past mainly using sialic acid binding lectin biosensors. [25][26][27]30 Incubation of the SNA lectin biosensor with asialofetuin, a non-sialic acid expressing glycoform variant of glycoprotein fetuin, showed very minimal change in the baseline impedance. [25][26][27]30 In contrast, a linear increase from baseline impedance was observed when the SNA lectin biosensor was incubated with an increasing concentration of fetuin expressing a correct glycan epitope.…”

Section: Glycoprotein Capture and Detectionmentioning

confidence: 99%

“…[25][26][27]30 Incubation of the SNA lectin biosensor with asialofetuin, a non-sialic acid expressing glycoform variant of glycoprotein fetuin, showed very minimal change in the baseline impedance. [25][26][27]30 In contrast, a linear increase from baseline impedance was observed when the SNA lectin biosensor was incubated with an increasing concentration of fetuin expressing a correct glycan epitope. [25][26][27]30 Apart from showing very high binding specicity towards the glycan epitope, these SNA lectin biosensors have demonstrated sensitivity for glycoprotein detection (e.g.…”

Section: Glycoprotein Capture and Detectionmentioning

confidence: 99%

“…25,27 This 10 pg mL À1 lower limit of detection in a 1000 fold diluted serum sample will actually translate into 10 ng mL À1 in the actual undiluted sample.…”

mentioning

confidence: 99%

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Electrochemical detection of glycan and protein epitopes of glycoproteins in serum

Shah

Hill

Shiddiky

et al. 2014

Analyst

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Aberrant protein glycosylation is associated with a range of pathological conditions including cancer and possesses diagnostic importance. Translation of glycoprotein biomarkers will be facilitated by the development of a rapid and sensitive analytical platform that simultaneously interrogates both the glycan and protein epitopes of glycoproteins in body fluids such as serum or saliva. To this end, we developed an electrochemical biosensor based on the immobilization of a lectin on the gold electrode surface to recognize/capture a target glycan epitope conjugated to glycoproteins, followed by detection of the protein epitope using a target protein-specific antibody. Electrochemical signals are generated by labelfree voltammetric or impedimetric interrogation of a ferro/ferricyanide redox couple (e.g. [Fe(CN) 6 ] 3À/4À ) on the sensing surface, where the change in voltammetric current or interfacial electron transfer resistance was measured. The detection system was demonstrated using the model glycoprotein chicken ovalbumin with Sambucus nigra agglutinin type I (SNA lectin), and exhibits femtomolar sensitivity in the background of diluted human serum. The results obtained in this proof-of-concept study demonstrate the possibility of using electrochemical detection for developing cheap point-of-care diagnostics with high specificity and sensitivity for blood glycoprotein biomarkers.

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Section: Glycoprotein Capture and Detectionmentioning

confidence: 93%

Section: Glycoprotein Capture and Detectionmentioning

confidence: 99%

Section: Glycoprotein Capture and Detectionmentioning

confidence: 99%

“…25,27 This 10 pg mL À1 lower limit of detection in a 1000 fold diluted serum sample will actually translate into 10 ng mL À1 in the actual undiluted sample.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical detection of glycan and protein epitopes of glycoproteins in serum

Shah

Hill

Shiddiky

et al. 2014

Analyst

View full text Add to dashboard Cite

show abstract

“…For this experiment, we exploited a newly developed technology that enables to significantly reduce the volume of probe required for genes/proteins arrays [25][26][27]. This technology relies upon the precise, non-contact printing capabilities of a piezoelectric inkjet printer.…”

Section: Activepipette Piezoelectric Inkjet Technologymentioning

confidence: 99%

Nanoprobe NAPPA Arrays for the Nanoconductimetric Analysis of Ultra-Low-Volume Protein Samples Using Piezoelectric Liquid Dispensing Technology

Pechkova¹,

Wiktor²,

Bragazzi³

et al. 2015

NanoWorld J

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In the last years, the evolution and the advances of the nanobiotechnologies applied to the systematic study of proteins, namely proteomics, both structural and functional, and specifically the development of more sophisticated and largescale protein arrays, have enabled scientists to investigate protein interactions and functions with an unforeseeable precision and wealth of details. Here, we present a further advancement of our previously introduced and described Nucleic Acid Programmable Protein Arrays (NAPPA)-based nanoconductometric sensor. We coupled Quartz Crystal Microbalance with Dissipation factor Monitoring (QCM_D) with piezoelectric inkjet printing technology (namely, the newly developed ActivePipette), which enables to significantly reduce the volume of probe required for genes/proteins arrays. We performed a negative control (with master mix, or MM) and a positive control (MM_p53 plus MDM2). We performed this experiment both in static and in flow, computing the apparent dissociation constant of p53-MDM2 complex (130 nM, in excellent agreement with the published literature). We compared the results obtained with the ActivePipette printing and dispensing technology vs. pin spotting. Without the ActivePipette, after MDM2 addition the shift in frequency (Δf ) was 7575 Hz and the corresponding adsorbed mass was 32.9 µg. With the ActivePipette technology, after MDM2 addition Δf was 7740 Hz and the corresponding adsorbed mass was 33.6 µg. With this experiment, we confirmed the sensing potential of our device, being able to discriminate each gene and protein as well as their interactions, showing for each one of them a unique conductance curve. Moreover, we obtained a better yield with the ActivePipette technology.

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Sensor Ecosystems for Health Self‐Monitoring: Focus on Diabetes Management

Ganguly,

Churcher,

Upasham

et al. 2023

Organic and Inorganic Materials Based Sensors

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Nanomonitor: A Miniature Electronic Biosensor for Glycan Biomarker Detection

Abstract: Based on its performance metrics, the NanoMonitor has excellent potential for development as a point-of-care handheld electronic biosensor device for routine detection of glycan biomarkers from clinical samples.

Cited by 63 publications

References 31 publications

Electrochemical detection of glycan and protein epitopes of glycoproteins in serum

Electrochemical detection of glycan and protein epitopes of glycoproteins in serum

Nanoprobe NAPPA Arrays for the Nanoconductimetric Analysis of Ultra-Low-Volume Protein Samples Using Piezoelectric Liquid Dispensing Technology

Sensor Ecosystems for Health Self‐Monitoring: Focus on Diabetes Management

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