Nanonized black soybean enhances immune response in senescence-accelerated mice

Abstract

Soy isofl avones may have applications in cancer prevention and anti-infl ammation, therefore this study was conducted to investigate the effect of dietary supplementation with black soybean on the immune response in the senescence-accelerated-prone mice (SAMP8) and -resistant mice (SAMPR1, as controls). The mechanism of isofl avones was also investigated. Six-month-old male SAMP8 and SAMR1 mice were divided into the control groups and experimental groups supplemented with nanonized (Nano-soy) or microparticled (Micro-soy) black soybeans (n = 8/group), respectively for 12 weeks. Human peripheral blood mononuclear cells (PBMC) and murine splenocytes were stimulated with mitogens and cytokines were determined by reverse transcriptase-polymerase chain reaction and/or ELISA. The results showed that body weight, food intake, and relative weights of organs did not differ among the SAMP8 control and experimental groups. Isofl avone (daidzin and genistin) intake was higher in the Nano-soy group than the Micro-soy group. The lymphoproliferation and production of interleukin-2 (IL-2) and interferon-gamma (IFN-γ) in the Nano-soy group had a signifi cantly higher (P Ͻ 0.05) than those in the control and Micro-soy groups. The Nano-soy supplemented mice reached these cytokine levels similar to SAMR1 mice. This result was consistent with the in vitro data that daidzein (a metabolite of daidzin), at a concentration of 10 μM, increased IL-2, IL-4, and IFN-γ production from phytohemagglutinin-stimulated PBMC (P Ͻ 0.05). However at higher concentrations (Ͼ50 μM), daidzein only reduced IL-10 and IFN-γ levels, whereas genistein reduced levels of the IL-2, IL-4, IL-10, IFN-γ mRNA and protein and these results suggest that the Nano-soy supplementation improved immune response in SAMP8 mice which may be attributable to higher daidzin content in the black soybean preparation.

Isoflavones-Enriched Soy Protein PreventsCCL4-Induced Hepatotoxicity in Rats

“…One of the mechanisms for lowering AFP level may have been the anti-inflammatory properties of soy. Previous studies have indicated that the isoflavones in soy may regulate the inflammatory response and immune function [ 64 , 65 ]. Moreover, genistein and secoisolariciresinol have anticancer properties on MCF-7 and BT20 in vitro [ 66 ] and have been suggested as the most potent inhibitors of cancer cell growth [ 67 ].…”