Nanoparticle-based biosensors for detection of pathogenic bacteria

Sanvicens, Nuria; Pastells, Carme; Pascual, Núria; Marco, M.‐Pilar

doi:10.1016/j.trac.2009.08.002

Cited by 229 publications

(118 citation statements)

References 66 publications

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“…Applications range from fields such as human and animal health (Ivnitski et al, 1999;Lazcka et al, 2007;Luppa et al, 2011;Sanvicens et al, 2009;Schmitt and Henderson, 2005;Tothill, 2001;Wilson and Gifford, 2005), food safety (Amine et al, 2006;Arora et al, 2011;Ivnitski et al, 2000;Leonard et al, 2003;Mello and Kubota, 2002;O'Kennedy et al, 2005;Patel, 2002;Van Dorst et al, 2010;Velusamy et al, 2010), environmental monitoring, including biological threat agents detection (Amine et al, 2006;Iqbal et al, 2000;Rodriguez-Mozaz et al, 2004Rogers, 1995;Rogers and Gerlach, 1999;Van Dorst et al, 2010;Velasco-Garcia and Mottram, 2003) to analysis of drinking water quality (Leonard et al, 2003;Mello and Kubota, 2002;Noble and Weisberg, 2005;O'Kennedy et al, 2005). Among the large number of existing biosensor types, e.g.…”

Section: Introductionmentioning

confidence: 99%

Turn-on optomagnetic bacterial DNA sequence detection using volume-amplified magnetic nanobeads

Bejhed

Torre

Donolato

et al. 2015

Biosensors and Bioelectronics

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Detection of a Vibrio cholerae DNA-sequence using an optomagnetic read-out exploiting the dynamic behavior of magnetic nanobeads along with two turn-on data analysis approaches is demonstrated. The optomagnetic method uses a weak uniaxial AC magnetic field of varying frequency applied perpendicular to the optical path and measures the modulation of laser light passing through a cuvette containing the sample with oligonucleotide-tagged magnetic beads and macromolecular coils of single-stranded DNA. The DNA coils are formed upon a padlock probe ligation followed by rolling circle amplification (RCA). The presence of target gives rise to a change of the 2nd harmonic component, V2=V2(')+iV2(''), of the transmitted light. We demonstrate that by using the phase angle ξ defined as ξ=arctanV2(')/V2('') in the low-frequency region we obtain a limit of detection of 10pM for an RCA time of only 20min corresponding to a total assay time of 60min. Moreover, we show that the approach based on ξ is significantly more robust than the analysis based on a turn-off of the signal due to free magnetic nanobeads used in previous work (Donolato et al., submitted for publication), where a limit of detection of 10pM was obtained for an RCA time of 60min. The increased robustness and the reduction in total assay time constitute significant steps towards the realization of a low-cost, rapid and sensitive biosensor platform suitable for pathogen detection in both human and veterinary medicine settings.

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Section: Introductionmentioning

confidence: 99%

Turn-on optomagnetic bacterial DNA sequence detection using volume-amplified magnetic nanobeads

Bejhed

Torre

Donolato

et al. 2015

Biosensors and Bioelectronics

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“…Their implementation in bioanalytical assays enhanced assay performance, opened new possibilities for devices miniaturization and allowed the development of entirely new assay formats. In particular, nanomaterials have generated great expectations with respect to increasing assay detectability and selectivity and reducing assay times [72][73][74][75][76] and to enhance analytical performance in a variety of analytical applications, such as microfluidic platforms [77], electrochemical [78], optical [79], and microgravimetry [80] biosensors, thus potentially providing significant advancements for multipathogen detection.…”

Section: Nanotechnologiesmentioning

confidence: 99%

Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection

et al. 2012

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Foodborne illnesses caused by pathogenic bacteria represent a widespread and growing problem to public health, and there is an obvious need for rapid detection of food pathogens. Traditional culture-based techniques require tedious sample workup and are time-consuming. It is expected that new and more rapid methods can replace current techniques. To enable large scale screening procedures, new multiplex analytical formats are being developed, and these allow the detection and/or identification of more than one pathogen in a single analytical run, thus cutting assay times and costs. We review here recent advancements in the field of rapid multiplex analytical methods for foodborne pathogenic bacteria. A variety of strategies, such as multiplex polymerase chain reaction assays, microarray-or multichannel-based immunoassays, biosensors, and fingerprint-based approaches (such as mass spectrometry, electronic nose, or vibrational spectroscopic analysis of whole bacterial cells), have been explored. In addition, various technological solutions have been adopted to improve detectability and to eliminate interferences, although in most cases a brief pre-enrichment step is still required. This review also covers the progress, limitations and future challenges of these approaches and emphasizes the advantages of new separative techniques to selectively fractionate bacteria, thus increasing multiplexing capabilities and simplifying sample preparation procedures.

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“…It is already known that these unique types of nanostructured matter have many advantages such as increased ratio surface/volume, controllable size, enhanced magnetic properties and they are easy to immobilize to an electrode surface by applying a magnetic field. Thus, various types of nanostructures have been engineered in order to detect specific targets, including bacteria [5][6][7][8][9]. For example, Koets et al, 2009 [10] developed a magneto-resistant sensor using superparamagnetic particles as labels for the detection of Escherichia coli and Salmonella.…”

Section: Introductionmentioning

confidence: 99%

Chemically modified nanoparticles surface for sensing bacterial loading—experimental study based on fluorescence stimulation by iron ions

Poiată

Creangă

Nădejde

et al. 2013

Bioelectrochemistry

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Nanoparticle-based biosensors for detection of pathogenic bacteria

Cited by 229 publications

References 66 publications

Turn-on optomagnetic bacterial DNA sequence detection using volume-amplified magnetic nanobeads

Turn-on optomagnetic bacterial DNA sequence detection using volume-amplified magnetic nanobeads

Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection

Chemically modified nanoparticles surface for sensing bacterial loading—experimental study based on fluorescence stimulation by iron ions

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