Nanoparticle-based Caspase Sensors

Maysinger, Dušica; Hutter, Eliza

doi:10.2217/nnm.14.158

Cited by 11 publications

(9 citation statements)

References 77 publications

(97 reference statements)

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“…However, nanotechnological probes are often large and cannot reach desirable intracellular locations. In addition, these probes are complex and relatively little is known about their stability in vivo , as well as their pharmacokinetics and pharmacodynamics ( 186 , 187 ). It is well established that the biological identity of a nanoparticle is distinct from its well-defined chemical identity.…”

Section: Discussionmentioning

confidence: 99%

“…Glia cells were also activated by gold nanoparticles, depending on the nanoparticles’ morphology ( 10 ). Activation of glia cells is often accompanied by the activation of inflammatory caspases and caspases implicated in apoptosis ( 187 ). Nanosensors for caspases have been developed, and examples of constructs for these sensors are illustrated in Figure 5 ( 170 , 188 ).…”

Section: Intravital Imaging Of Neuroinflammationmentioning

confidence: 99%

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Nutritional and Nanotechnological Modulators of Microglia

Maysinger

Zhang

2016

Front. Immunol.

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Microglia are the essential responders to alimentary, pharmacological, and nanotechnological immunomodulators. These neural cells play multiple roles as surveyors, sculptors, and guardians of essential parts of complex neural circuitries. Microglia can play dual roles in the central nervous system; they can be deleterious and/or protective. The immunomodulatory effects of alimentary components, gut microbiota, and nanotechnological products have been investigated in microglia at the single-cell level and in vivo using intravital imaging approaches, and different biochemical assays. This review highlights some of the emerging questions and topics from studies involving alimentation, microbiota, nanotechnological products, and associated problems in this area of research. Some of the advantages and limitations of in vitro and in vivo models used to study the neuromodulatory effects of these factors, as well as the merits and pitfalls of intravital imaging modalities employed are presented.

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Section: Discussionmentioning

confidence: 99%

Section: Intravital Imaging Of Neuroinflammationmentioning

confidence: 99%

Nutritional and Nanotechnological Modulators of Microglia

Maysinger

Zhang

2016

Front. Immunol.

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“…ZnS-capped CdSe QDs have been shown to be sensitive to environmental factors such as pH and the presence of divalent cations [58]. QDs-based sensors utilizing the fluorescent resonance energy transfer (FRET) technique deserve a special interest, as they can be applied as ratiometric cellular probes for the measurement of pH, oxygen, metal ions and enzymatic activity [13,59], including caspases as important indicators of cell health and apoptotic processes [60].…”

Section: Quantum Dots For In Vitro Biomedical Experimental Systemsmentioning

confidence: 99%

Quantum dots in nanomedicine: recent trends, advances and unresolved issues

Volkov

2015

Biochemical and Biophysical Research Communications

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a b s t r a c tThe review addresses the current state of progress in the use of ultra-small nanoparticles from the category of quantum dots (QDs), which presently embraces a widening range of nanomaterials of different nature, including "classical" semiconductor groups III-V and II-VI nanocrystals, along with more recently emerged carbon, silicon, gold and other types of nanoparticles falling into this class of nanomaterials due to their similar physical characteristics such as small size and associated quantum confinement effects. A diverse range of QDs applications in nanomedicine has been extensively summarised previously in numerous publications. Therefore, this review is not intended to provide an all-embracing survey of the well documented QDs uses, but is rather focused on the most recent emerging developments, concepts and outstanding unresolved problematic and sometimes controversial issues. Over 125 publications are overviewed and discussed here in the context of major nanomedicine domains, i.e. medical imaging, diagnostics, therapeutic applications and combination of them in multifunctional theranostic systems.© 2015 Elsevier Inc. All rights reserved. Quantum dots in nanomedicine: recent trendsWithin a steadily increasing database of diverse nanomaterials reported as suitable for applications in nanomedicine [1e10], quantum dots (QDs) deservedly occupy a special niche as nanoparticles with a unique track record full of high expectations, dramatic pitfalls and often controversial experimental evidence. From the early optimistic aspirations of them as breakthrough tools for multipurpose in vitro and in vivo applications [11e17], they have been subject to a period of partial relinquishment following the sombre realisation that in the original unmodified state QDs did not stand a chance to deserve a unanimous approval as imaging or drug delivery vehicles in humans, due to their intrinsic toxicity and lack of biodegradability perspective. The disappointingly low yield of exploitable outputs following the initial rounds of heavy investments in the field has also contributed to the overall decline in research productivity metrics. The analysis of the recent ten years' trends in publications number in this area related to the QDs medical applications in vivo in general (Fig. 1 A), as well as for imaging and diagnostics purposes (Fig. 1 B, C) according to Thomson Reuters Web of Science™ database clearly reflects such "cooling down" period after 2010 resulting in a significant reduction in the initially steady exponential growth of publication rates. This trend has been partially or completely reversed by 2014 and it is intriguing whether we will see it sustained in 2015 and beyond.Such returning enthusiasm has been reinvigorated by a number of objective tendencies in the related technological developments, including the arrival of innovative nanotechnology-enabled tools for diagnostic and ex vivo imaging applications [18,19], the arrival of new types of QDs of alternative nature offering the opportunities of r...

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“…TPL has been employed mainly for bioimaging (Vo-Dinh et al, 2013 ; Yellen and Mongeon, 2015 ), but it should be considered for detection of changes in enzymatic activities in neural cells under physiological and pathological conditions. Luminescent AuNPs with high quantum yields are attractive candidates to replace QDs or some organic fluorophores (Maysinger and Hutter, 2015 ). A “plasmonic resonance energy transfer (PRET)” can be exploited for measurements of enzymes engaged in disrupted redox homeostasis, neuroinflammation and protein shedding (cleavage of the ectodomain of membrane proteins; Altmeppen et al, 2013 ; Saftig and Bovolenta, 2015 ).…”

Section: Looking Ahead To New Ways To Sense Neural Cells Under Physiomentioning

confidence: 99%

Nanoparticle-Based and Bioengineered Probes and Sensors to Detect Physiological and Pathological Biomarkers in Neural Cells

Maysinger

Hutter

et al. 2015

Front. Neurosci.

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Nanotechnology, a rapidly evolving field, provides simple and practical tools to investigate the nervous system in health and disease. Among these tools are nanoparticle-based probes and sensors that detect biochemical and physiological properties of neurons and glia, and generate signals proportionate to physical, chemical, and/or electrical changes in these cells. In this context, quantum dots (QDs), carbon-based structures (C-dots, grapheme, and nanodiamonds) and gold nanoparticles are the most commonly used nanostructures. They can detect and measure enzymatic activities of proteases (metalloproteinases, caspases), ions, metabolites, and other biomolecules under physiological or pathological conditions in neural cells. Here, we provide some examples of nanoparticle-based and genetically engineered probes and sensors that are used to reveal changes in protease activities and calcium ion concentrations. Although significant progress in developing these tools has been made for probing neural cells, several challenges remain. We review many common hurdles in sensor development, while highlighting certain advances. In the end, we propose some future directions and ideas for developing practical tools for neural cell investigations, based on the maxim “Measure what is measurable, and make measurable what is not so” (Galileo Galilei).

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Nanoparticle-based Caspase Sensors

Cited by 11 publications

References 77 publications

Nutritional and Nanotechnological Modulators of Microglia

Nutritional and Nanotechnological Modulators of Microglia

Quantum dots in nanomedicine: recent trends, advances and unresolved issues

Nanoparticle-Based and Bioengineered Probes and Sensors to Detect Physiological and Pathological Biomarkers in Neural Cells

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