Nanoparticle-based drug delivery systems for cancer therapy

Dang, Yu; Guan, Jianjun

doi:10.1016/j.smaim.2020.04.001

Cited by 381 publications

(185 citation statements)

References 80 publications

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“…For characterization of PKAs- and BSAs-loaded CRISPRMAX complexes, dynamic light scattering (DLS) measurements were performed for analyzing the particle size, polydispersity index (PDI), and zeta potential of the enzymes-loaded CRISPRMAX complexes. In in vitro studies of using LNPs in therapeutics delivery, lipid-based particles with a PDI value of 0.3 or below are considered to be acceptable carriers for delivering therapeutics, as such vesicles are homogeneously distributed in the solution [ 50 , 51 ]; the PDI values of our enzymes-loaded CRISPRMAX complexes and null CRISPRMAX were also within this range, which indicated these complexes were evenly distributed in the solution. In addition, the average particle size of CRISPRMAX-PKAs, CRISPRMAX-BSAs, and null CRISPRMAX was approximately 350 nm; these complexes can be easily internalized by targeted cells, since their size is less than 500 nm [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Delivery of Protein Kinase A by CRISPRMAX and Its Effects on Breast Cancer Stem-Like Properties

et al. 2020

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Protein kinase A (PKA) activation has recently been reported to inhibit epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) ability, which is considered to be responsible for chemoresistance and tumor recurrence in patients. While current studies mainly focus on gene manipulation of the EMT process, the direct delivery of PKA enzymes to cancer cells has never been investigated. Here, we utilize the commercial Lipofectamine CRISPRMAX reagent to directly deliver PKAs to breast cancer cells and evaluate its effects on EMT regulation. We optimized the delivery parameters with fluorescent-labeled bovine serum albumin, and successfully delivered fluorescent PKAs through CRISPRMAX into breast cancer cells. Then, we evaluated the biological effects by immunofluorescence, flow cytometry, mammosphere assay, and chemoresistance assay. Our data showed the expression of EMT-related markers, α-smooth muscle actin and N-cadherin, was downregulated after CRISPRMAX-PKA treatment. Although the CD44+/CD24− population did not change considerably, the size of mammospheres significantly decreased. In paclitaxel and doxorubicin chemoresistance assays, we noticed PKA delivery significantly inhibited paclitaxel resistance rather than doxorubicin resistance. Taken together, these results suggest our direct enzyme delivery can be a potential strategy for inhibiting EMT/CSC-associated traits, providing a safer approach and having more clinical translational efficacy than gene manipulation. This strategy will also facilitate the direct testing of other target enzymes/proteins on their biological functions.

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Section: Discussionmentioning

confidence: 99%

Delivery of Protein Kinase A by CRISPRMAX and Its Effects on Breast Cancer Stem-Like Properties

et al. 2020

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“…The advancement and innovations in the field of nanotechnology has made nanoparticles (NPs) delivery system as a promising approach to address the bottlenecks associated with the conventional therapies. [ 5 ] NP based delivery platform aim to ameliorate the therapeutic index of drugs by fine‐tuning their release profile, biodistribution, and pharmacokinetics. [ 6 ] Although NPs follow passive tumor accumulation through enhanced permeability and retention (EPR) effect, the clinical relevance is being questioned.…”

Section: Introductionmentioning

confidence: 99%

Targeted Theranostic Nano Vehicle Endorsed with Self‐Destruction and Immunostimulatory Features to Circumvent Drug Resistance and Wipe‐Out Tumor Reinitiating Cancer Stem Cells

Joseph

Ramya

Vijayan

et al. 2020

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The downsides of conventional cancer monotherapies are profound and enormously consequential, as drug‐resistant cancer cells and cancer stem cells (CSC) are typically not eliminated. Here, a targeted theranostic nano vehicle (TTNV) is designed using manganese‐doped mesoporous silica nanoparticle with an ideal surface area and pore volume for co‐loading an optimized ratio of antineoplastic doxorubicin and a drug efflux inhibitor tariquidar. This strategically framed TTNV is chemically conjugated with folic acid and hyaluronic acid as a dual‐targeting entity to promote folate receptor (FR) mediated cancer cells and CD44 mediated CSC uptake, respectively. Interestingly, surface‐enhanced Raman spectroscopy is exploited to evaluate the molecular changes associated with therapeutic progression. Tumor microenvironment selective biodegradation and immunostimulatory potential of the MSN‐Mn core are safeguarded with a chitosan coating which modulates the premature cargo release and accords biocompatibility. The superior antitumor response in FR‐positive syngeneic and CSC‐rich human xenograft murine models is associated with a tumor‐targeted biodistribution, favorable pharmacokinetics, and an appealing bioelimination pattern of the TTNV with no palpable signs of toxicity. This dual drug‐loaded nano vehicle offers a feasible approach for efficient cancer therapy by on demand cargo release in order to execute complete wipe‐out of tumor reinitiating cancer stem cells.

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“…Controlled drug delivery systems are considered beneficial in cancer therapies due to improved efficacy and lower side effects. [ 3,4 ] Following the first report by Lee and co‐workers of the preparation of polydopamine (PDA) via simple oxidative self‐polymerization of dopamine in 2007, [ 5 ] PDA has been the focus of considerable scientific attention due to its inherent advantages, such as good biocompatibility, degradability, low toxicity, ease of functionalization, and synthesis. [ 6–9 ] PDA has been applied in the biomedical field that for drug delivery, bioimaging, and tissue engineering biosensing applications.…”

Section: Introductionmentioning

confidence: 99%

Mussel‐Inspired Polydopamine: The Bridge for Targeting Drug Delivery System and Synergistic Cancer Treatment

Wang

Tang

Zhang

et al. 2020

Macromolecular Bioscience

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PDA has been applied in the biomedical field that for drug delivery, bioimaging, and tissue engineering biosensing applications. [10-12] Because of its specific structure, PDA is capable of loading antitumor drugs (such as doxorubicin (DOX)) via π-π stacking or hydrogen bonding interactions, with loading rates as high as 91%. Surprisingly, in addition to its strong ability to adsorb drugs, PDA is effective in releasing drugs by internal stimulation (hydrogen peroxide) or from an external stimulus (nearinfrared (NIR) irradiation), reducing the toxic effects of chemotherapeutic drugs. [13-15] Moreover, by exhibiting 40% light-to-heat conversion efficiency, PDA can act as a photothermal agent, able to produce sufficient heat to ablate cancer cells when irradiated with 808 nm NIR light. Compared with other photothermal materials, including carbon or copper-based nanomaterials and gold nanoparticles, the photothermal conversion effect of PDA is greater with better biocompatibility. [16-20] It has been reported that monotherapy has multiple limitations due to its heterogeneity and the drug resistance of tumors, with synergistic therapies that are more effective for cancer treatment. [21] Because of the large number of catechol and amine groups, PDA displays superior adhesion, endowing PDA films with the ability to adhere to a variety of material surfaces, such as gold nanoparticles, iron oxide, silica, and polylactic-co-glycolic acid (PLGA). Thus, PDA has been utilized to functionalize almost every form of nanomaterial by dopamine polymerization. More importantly, a layer of PDA provides nanomaterials with highly desirable properties, including the ability to be loaded with drugs allowing their controlled-release, excellent photothermal conversion efficiency and biocompatibility, and so facilitating the creation of multifunctional drug delivery systems for combined treatment by combining photothermal therapy (PTT) or chemotherapy with other therapies. [22-25] Additionally, the catechol and amino groups on PDA provide a bridge for subsequent modification. For example, PDA can act as a link to introduce tumor-targeting ligands using its amine and thiol groups via a Michael addition or Schiff reaction, allowing nanoparticles to become enriched at the tumor site. [26-28] Using these properties of PDA, researchers have developed diverse forms of PDA-based multifunctional platforms for synergistic cancer therapies. Wang et al. [29] reviewed Polydopamine (PDA), a mussel-inspired molecule, has been recognized as attractive in cancer therapy due to a number of inherent advantages, such as good biocompatibility, outstanding drug-loading capacity, degradability, superior photothermal conversion efficiency, and low tissue toxicity. Furthermore, due to its strong adhesive property, PDA is able to functionalize various nanomaterials, facilitating the construction of a PDA-based multifunctional platform for targeted or synergistic therapy. Herein, recent PDA research, including targeted drug delivery, single-mode therapy, and diverse...

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Nanoparticle-based drug delivery systems for cancer therapy

Cited by 381 publications

References 80 publications

Delivery of Protein Kinase A by CRISPRMAX and Its Effects on Breast Cancer Stem-Like Properties

Delivery of Protein Kinase A by CRISPRMAX and Its Effects on Breast Cancer Stem-Like Properties

Targeted Theranostic Nano Vehicle Endorsed with Self‐Destruction and Immunostimulatory Features to Circumvent Drug Resistance and Wipe‐Out Tumor Reinitiating Cancer Stem Cells

Mussel‐Inspired Polydopamine: The Bridge for Targeting Drug Delivery System and Synergistic Cancer Treatment

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