Nanoparticle-based Optical Detection of MicroRNA

Zhang, Jingpu; Cui, Daxiang

doi:10.5101/nbe.v5i1.p1-10

Cited by 13 publications

(5 citation statements)

References 67 publications

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“…Electrochemical DNA hybridization sensors have potential as detection techniques in a POC test because this technology can detect specific miRNAs in the attomolar range without PCR amplification . Other promising miRNA detection methods include nanoparticle‐based optical technologies , surface plasmon resonance and amplification‐free fluorescence‐based assays . As miR‐122 is a relatively high‐concentration, organ‐specific, circulating miRNA with a large dynamic range in disease it represents an ideal target for assay development with line of sight on a commercial product tackling a global health need.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐122 can be measured in capillary blood which facilitates point‐of‐care testing for drug‐induced liver injury

Vliegenthart¹,

Berends²,

Potter³

et al. 2017

Brit J Clinical Pharma

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AimsLiver‐enriched microRNA‐122 (miR‐122) is a novel circulating biomarker for drug‐induced liver injury (DILI). To date, miR‐122 has been measured in serum or plasma venous samples. If miR‐122 could be measured in capillary blood obtained from a finger prick it would facilitate point‐of‐care testing, such as in resource‐limited settings that have a high burden of DILI.MethodsIn this study, in healthy subjects, miR‐122 was measured by polymerase chain reaction in three capillary blood drops taken from different fingers and in venous blood and plasma (n = 20). miR‐122 was also measured in capillary blood obtained from patients with DILI (n = 8).ResultsCirculating miR‐122 could be readily measured in a capillary blood drop in healthy volunteers with a median (interquartile range) cycle threshold (Ct) of 32.6 (31.1–34.2). The coefficient of variation for intraindividual variability across replicate blood drops was 49.9%. Capillary miR‐122 faithfully reflected the concentration in venous blood and plasma (Pearson R = 0.89, P < 0.0001; 0.88, P < 0.0001, respectively). miR‐122 was 86‐fold higher in DILI patients [median value 1.0 × 108 (interquartile range 1.89 × 107–3.04 × 109) copies/blood drop] compared to healthy subjects [1.85 × 106 (4.92 × 105–5.88 × 106) copies/blood drop]. Receiver operator characteristic analysis demonstrated that capillary miR‐122 sensitively and specifically reported DILI (area under the curve: 0.96, P = 0.0002).ConclusionThis work supports the potential use of miR‐122 as biomarker of human DILI when measured in a capillary blood drop. With development across DILI aetiologies, this could be used by novel point‐of‐care technologies to produce a minimally invasive, near‐patient, diagnostic test.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‐122 can be measured in capillary blood which facilitates point‐of‐care testing for drug‐induced liver injury

Vliegenthart¹,

Berends²,

Potter³

et al. 2017

Brit J Clinical Pharma

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“…As a result, optical methods are ideally suited to direct detection with minimal purification requirements. A diverse set of optical techniques are available, and as these are thoroughly reviewed elsewhere (25,85), our discussion is limited to recent enhancements that offer potential for increased sensitivity and selectivity using fairly standard techniques.…”

Section: Optical-based Detectionmentioning

confidence: 99%

MicroRNA Detection: Current Technology and Research Strategies

Hunt

Broyles

Head

et al. 2015

Annual Rev. Anal. Chem.

161

130

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The relatively new field of microRNA (miR) has experienced rapid growth in methodology associated with its detection and bioanalysis as well as with its role in -omics research, clinical diagnostics, and new therapeutic strategies. The breadth of this area of research and the seemingly exponential increase in number of publications on the subject can present scientists new to the field with a daunting amount of information to evaluate. This review aims to provide a collective overview of miR detection methods by relating conventional, established techniques [such as quantitative reverse transcription polymerase chain reaction (RT-qPCR), microarray, and Northern blotting (NB)] and relatively recent advancements [such as next-generation sequencing (NGS), highly sensitive biosensors, and computational prediction of microRNA/targets] to common miR research strategies. This should guide interested readers toward a more focused study of miR research and the surrounding technology.

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“…In the meantime, miRNAs are known to work as oncomiRs or tsmiRs [ 20 , 29 - 31 ]. Most miRNAs present the features of one of them.…”

Section: Introductionmentioning

confidence: 99%

“…It is essential to develop an efficient detection system for miRNAs and their target sequences not only in the basic research of life science but in the diagnosis and treatment of diseases [ 33 ]. However, miRNAs are small and feature a diverse copy number in cells from 50,000 copies to several thousand in a small fraction (about 1% of total RNAs), exhibiting sequence similarity within miRNA family members [ 31 , 34 - 36 ]. Such features are obstacles to the development of an efficient miRNA detection system.…”

Section: Introductionmentioning

confidence: 99%

A Combinational Approach for More Efficient miRNA Biosensing

Lee

2022

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A microRNAs, short single-stranded noncoding RNAs ranging in length from 18 ~ 24 bp, are found in all kingdoms of eukaryotes and even viruses. It was found that miRNAs got involved in a variety of biological phenomenon control and their intracellular aberrant expression was related to diseases and abnormalities in the immune system. Since then, it has been considered essential to develop an efficient miRNA detection system. In this review, the limitations of traditional scheme-based miRNA detection methods are compared and analyzed. In particular, nucleic acid amplification based miRNA detection methods and nanomaterial-based miRNA detection methods, which are widely used as a biosensing platform because of such features and advantages as high sensitivity, specificity, and simplicity were analyzed. Based on this analysis, the latest examples of combination of advantages of nucleic acid amplification and the features and advantages of nanomaterials are examined to suggest the characteristics of the next generation of miRNA biosensing.

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Nanoparticle-based Optical Detection of MicroRNA

Cited by 13 publications

References 67 publications

MicroRNA‐122 can be measured in capillary blood which facilitates point‐of‐care testing for drug‐induced liver injury

MicroRNA‐122 can be measured in capillary blood which facilitates point‐of‐care testing for drug‐induced liver injury

MicroRNA Detection: Current Technology and Research Strategies

A Combinational Approach for More Efficient miRNA Biosensing

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