Nanoparticle‐based pseudostationary phases in CEC: A breakthrough in protein analysis?

Nilsson, Christian; Birnbaum, Staffan; Nilsson, Staffan

doi:10.1002/elps.201000645

Cited by 30 publications

(20 citation statements)

References 66 publications

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“…The use of NP as a PSP for CEC has been thoroughly reviewed [67]. In the BGE, the NP can interact with the proteins during the separation, changing their electrophoretic mobility and generating a separation based on the difference in affinity between the analytes for the NP.…”

Section: Techniquesmentioning

confidence: 99%

“…In the BGE, the NP can interact with the proteins during the separation, changing their electrophoretic mobility and generating a separation based on the difference in affinity between the analytes for the NP. A wide range of materials have been investigated for PSP-CEC, including polymer NP, carbon nanotubes, gold NP, and silica NP (SNP) [67]. To improve the stability and functionality of SNP, Gao et al synthesized polyamidoamine-grafted SNP (PAMAM-SNP) and utilized them for a separation of basic and acidic proteins [68] (Fig.…”

Section: Techniquesmentioning

confidence: 99%

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Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis

2014

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The development of therapeutic proteins and peptides is an expensive and time-intensive process. Biologics, which have become a multi-billion dollar industry, are chemically complex products that require constant observation during each stage of development and production. Post-translational modifications along with chemical and physical degradation from oxidation, deamidation, and aggregation, lead to high levels of heterogeneity that affect drug quality and efficacy. The various separation modes of capillary electrophoresis (CE) are commonly utilized to perform quality control and assess protein heterogeneity. This review attempts to highlight the most recent developments and applications of CE separation techniques for the characterization of protein and peptide therapeutics by focusing on papers accepted for publication in the in the two-year period between January 2012 and December 2013. The separation principles and technological advances of CE, capillary gel electrophoresis, capillary isoelectric focusing, capillary electrochromatography and CE-mass spectrometry are discussed, along with exciting new applications of these techniques to relevant pharmaceutical issues. Also included is a small selection of papers on microchip electrophoresis to show the direction this field is moving with regards to the development of inexpensive and portable analysis systems for on-site, high-throughput analysis.

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Section: Techniquesmentioning

confidence: 99%

Section: Techniquesmentioning

confidence: 99%

Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis

2014

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“…The main advantage of polymeric nanoparticles over conventional micelles as pseudo‐stationary phase in EKC is the possibility to use MS detection . The applicability of lipid‐based liquid crystalline nanoparticles as carriers in EKC was discussed in a recent review on the use of nanoparticles in capillary electromigration techniques .…”

Section: Lipid‐based Nanoparticles In Ekcmentioning

confidence: 99%

Capillary electromigration techniques for studying interactions between analytes and lipid dispersions

Wiedmer

Lokajová

2012

J of Separation Science

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CE has matured into a well-known and much used separation technique, with applications covering more or less all types of samples. EKC, which originally was developed for the separation of uncharged compounds, is still today under much development, with main focus on finding the perfect or ideal carriers (pseudo-stationary phase) for a broad range of analytes. In this review, the use of lipid dispersions as pseudostationary phases in EKC performed in capillaries and microchips, in addition to CE frontal analysis and partial filling EKC using lipid dispersions is discussed. Various types of lipid dispersions including liposomes, PEG-stabilized aggregates, proteoliposomes, lipid-based nanoparticles, and commercial lipid emulsions are described. The purpose of the review is to give the reader an overview of how EKC, CE frontal analysis, and partial filling EKC, have been applied to the study of interactions between analytes and lipid membranes.

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“…Nilsson et al . reviewed nanoparticle‐based pseudostationary phases in CEC with special emphasis on protein analysis .…”

Section: Introductionmentioning

confidence: 99%

Recent highlights in electro‐driven separations‐ selected applications of alkylthiol gold nanoparticles in capillary electrophoresis and capillary electro‐chromatography

Guihen

2017

Electrophoresis

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To date, alkylthiol gold nanoparticles (AuNPs) have been widely used in electro-chromatographic separation techniques as a viable alternative to traditional stationary phases. This is mainly due to their stability, chemical inertness, ease of functionality, increased phase ratio, ability to form self-assembled monolayers. They also yield versatile stationary phases with highly specific targeted functionalities. At the nanoscale region, the chemical and physical properties of a molecule display different attributes to that of the parent molecules or material, hence these features can be harnessed in electro-driven chromatographic separations. Application areas illustrating the use of AuNPs in separation science continue to grow and expand to cover many different kinds of analysis. The last decade has witnessed a successful trend in miniaturisation of chemical separation systems toward the micro and nanoscale ranges. Nanoparticle-based stationary phases fit well with performing chemical separations on microfluidic and capillary platforms. In this review the theory of the use of alkylthiol gold nanoparticles in electro-chromatographic driven separation methods will be discussed. This will be followed by details of recent and selected applications showing alkylthiol gold nanoparticles in capillary electrophoretic and open-tubular electro-chromatographic separations. This review will focus solely on alkylthiol based gold nanoparticles, therefore other kinds of chemical moieties bonded to gold nanoparticles are outside the scope of this review. Finally the future outlook of this exciting technology will be outlined in some detail in the final section.

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Nanoparticle‐based pseudostationary phases in CEC: A breakthrough in protein analysis?

Cited by 30 publications

References 66 publications

Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis

Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis

Capillary electromigration techniques for studying interactions between analytes and lipid dispersions

Recent highlights in electro‐driven separations‐ selected applications of alkylthiol gold nanoparticles in capillary electrophoresis and capillary electro‐chromatography

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