Nanoparticle corona for proteins: mechanisms of interaction between dendrimers and proteins

Shcharbin, Dzmitry; Йонов, Максим; Abashkin, Viktar; Loznikova, Svetlana; Dzmitruk, Volha; Shcharbina, Natallia; Matusevich, Ludmila; Miłowska, Katarzyna; Gałęcki, Krystian; Wysocki, S.; Bryszewska, Maria

doi:10.1016/j.colsurfb.2015.07.017

Cited by 34 publications

(22 citation statements)

References 34 publications

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“…However, the combined exposure of TiO 2 NPs with food nutrients and the potential interactions have not been adequately investigated. Indeed, interaction between NPs and proteins, which was called NP‐protein corona, has long been discovered and confirmed that it could determine the biological properties of NPs (Choi, Riviere, & Monteiro‐Riviere, ; Konduru et al, ; Shcharbin et al, ; Wu et al, ). The interaction of NPs with lipid was also been proposed in a recent study (Montis, Maiolo, Alessandri, Bergese, & Berti, ).…”

Section: Introductionmentioning

confidence: 96%

Combined effect of titanium dioxide nanoparticles and glucose on the cardiovascular system in young rats after oral administration

Chen

Zhou

Wang

et al. 2018

J of Applied Toxicology

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Titanium dioxide nanoparticles (TiO2 NPs) have already been used as food additive in various products and are usually consumed with a considerable amount of sugar. Oral consumption of TiO2 NPs poses concerning health risks; however, research on the combined effect of ingested TiO2 NPs and glucose is limited. We examined young Sprague‐Dawley rats administrated TiO2 NPs orally at doses of 0, 2, 10 and 50 mg/kg body weight per day with and without 1.8 g/kg body weight glucose for 30 and 90 days. Heart rate, systolic and diastolic blood pressure, blood biochemical parameters and histopathology of cardiac tissues was assessed to quantify cardiovascular damage. The results showed that oral exposure to TiO2 NPs and high doses of glucose both could induce cardiovascular injuries. The toxic effects were dose‐, time‐ and gender‐dependent. The interaction effects between oral‐exposed TiO2 NPs and glucose existed and revealed to be antagonism in most of the biological parameters. However, toxic effects of the high‐dose glucose seemed to be more severe than TiO2 NPs and the interaction of TiO2 NPs with glucose. These results suggest that it may be more important to control the sugar intake than TiO2 NPs for protecting the health of TiO2 NP consumers.

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Section: Introductionmentioning

confidence: 96%

Combined effect of titanium dioxide nanoparticles and glucose on the cardiovascular system in young rats after oral administration

Chen

Zhou

Wang

et al. 2018

J of Applied Toxicology

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“…Serum albumins are the main transport proteins in plasma (50-60% of total plasma proteins), giving an FW of 66 kDa [16][17][18]. Albumins can bind anionic and cationic ligands with high affinity due to the presence of charged groups and hydrophobic pockets in their structure, and due to their negative surface charge [16][17][18][19][20]. Human alpha-1-microglobulin (A1M) is a glycoprotein of˜30 kDa present in blood plasma and some tissues of the human body [17,21].…”

Section: Introductionmentioning

confidence: 99%

Immunoreactivity changes of human serum albumin and alpha-1-microglobulin induced by their interaction with dendrimers

Serchenya

Shcharbin

Shyrochyna

et al. 2019

Colloids and Surfaces B: Biointerfaces

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Dendrimers are hyperbranched polymers for delivery of therapeutic genetic material to cancer cells. The fine tuning chemical modifications of dendrimers allow for the modification of the composition. The architecture and the properties of dendrimers are key factors to improve their in vitro and in vivo properties such as biocompatibility with cells and tissues and their pharmacokinetic/pharmacodynamic behavior. The side effects of dendrimers on structure and function of proteins is an important question that must be addressed. We herein describe the effect of newly synthesized piperidine-based cationic phosphorous dendrimers of 2 generations and commercial cationic, neutral and anionic poly(amidoamine) (PAMAM) dendrimers of 4th generation on immunochemical properties of 2 serum proteins: human serum albumin (HSA) and alpha-1-microglobulin (A1M). Both can bind and transfer ligands in blood, including hormones, fatty acids, toxins and drugs, and have immunoreactivity properties. Comparing the effects of piperidinium-terminated phosphorus and cationic, neutral and anionic PAMAM dendrimers on HSA and A1M, we conclude that, in the case of equimolar complexes, these dendrimers had no significant effect on immunoreactivity of proteins. In contrast, the formation of complexes in which a protein is fully bound to dendrimers leads to partial (1.2-2.3 times) reduction in protein immunoreactivity. The most important fact is that dendrimer-induced change in immunoreactivity of proteins is not complete, even if the protein is entirely bound by dendrimers. This means that the application of dendrimers in vivo will not totally hamper the immunoreactivity of these proteins and antibodies.

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“…In particular, βcyclodextrin (β-CD)-modified dendrimer-entrapped gold nanoparticles were applied for an improved delivery of small interfering RNA to glioblastomas [7]; a nuclear localization signal (NLS) of peptide-targeted gold nanoparticles with co-loaded anti-221 and AS1411 was used for the treatment of acute myeloid leukemia [4]; dendrimer-entrapped gold nanoparticles with co-loaded gemcitabine and miR-21 inhibitor were used for pancreatic cancer therapy [5]; chitosan stabilized goldfolate-poly(lactide-co-glycolide) complexes were used to treat hepatic and breast cancer cells [8] and arginine functionalized gold nanoparticles were used for delivery of CRISPR/Cas9-ribonucleoprotein into cytosol and the cell nucleus [9]. However, all AuNPs applied in vivo can interact with serum proteins in the bloodstream, firstly with serum albumins [10][11][12]. The interaction of nanoparticles with proteins (the 'protein corona') is a critical challenge when attempting to use nanoparticles in medicine.…”

Section: Introductionmentioning

confidence: 99%

“…Domains of HSA have net charges of −9 (domain I), −8 (domain II) and +2 (domain III) at pH 7.0 [16,17]. Albumins can bind anionic and cationic ligands with high affinity due to the presence of cationic groups and hydrophobic pockets in their structure [11,16,18]. The detailed steady-state and time-resolved fluorescence experiments, threedimensional fluorescence, FTIR, and circular dichroism data as well as molecular docking studies allowed to study in detail the interaction between albumin and pepsin and different compounds: in particular, it was found that human serum albumin can bind imidasole derivatives [19,20] and phenanthridine derivatives [21].…”

Section: Introductionmentioning

confidence: 99%

Role of cationic carbosilane dendrons and metallic core of functionalized gold nanoparticles in their interaction with human serum albumin

Shcharbin

Pedziwiatr-Werbicka

Serchenya

et al. 2018

International Journal of Biological Macromolecules

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Functionalization of gold nanoparticles by different chemical groups is an important issue regarding the biomedical applications of such particles. Therefore we have analyzed the interaction between gold nanoparticles functionalized by carbosilane dendrons with human serum albumin at different pHs, and in the presence of the protein unfolding agent, guanidine hydrochloride, using circular dichroism, zeta-potential and fluorescence quenching. The effect of a nanoparticle dendronization and pure dendrons on the immunoreactivity of albumin was estimated using ELISA. In addition, the tool to estimate the binding capacity of dendronized gold nanoparticles using a hydrophobic fluorescent probe 1,8-ANS (1-anilinonaphthalene-8-sulfonic acid) was chosen. We concluded that the effect of a nanoparticle on the structure, immunochemical properties and unfolding of albumin significantly decreased with second and third generations dendrons attached. Differences in pH dependence of the interaction between nanoparticles, their dendrons and albumin showed several effects of the "dendritic corona" and the metallic part of nanoparticle on the protein. These interactions indicate changes in the immunoreactivity of the protein, whereas dendron coating per se had no effect. Thus, dendronization of gold nanoparticles helps to shield them from interactions with plasma proteins.

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Nanoparticle corona for proteins: mechanisms of interaction between dendrimers and proteins

Cited by 34 publications

References 34 publications

Combined effect of titanium dioxide nanoparticles and glucose on the cardiovascular system in young rats after oral administration

Combined effect of titanium dioxide nanoparticles and glucose on the cardiovascular system in young rats after oral administration

Immunoreactivity changes of human serum albumin and alpha-1-microglobulin induced by their interaction with dendrimers

Role of cationic carbosilane dendrons and metallic core of functionalized gold nanoparticles in their interaction with human serum albumin

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