Nanoparticle-induced structural changes in lung surfactant membranes: an X-ray scattering study

Behyan, Shirin; Borozenko, Olga; Khan, Abdullah; Faral, Manon; Badia, Antonella; DeWolf, Christine

doi:10.1039/c8en00189h

Cited by 22 publications

(53 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This interaction, which occurs with an LS having an average negative charge, is enhanced as the charge density of the particles is increased [208]. The different impact of charged particles on the interfacial properties of lipid mixtures and LS formulations containing proteins was independently demonstrated by Behyan et al [189] in their study about the interaction of positively and negatively charged silicon dioxide particles with different LS models, a DPPC/POPG mixture, and an extract from calf lung (Infasurf). They found that whereas the behavior of Infasurf was only modified by the cationic particles, that of the natural extract was influenced by both, positively and negatively charged particles.…”

Section: Role Of Particle Surface Charge and Wettability On Their Int...mentioning

confidence: 94%

“…However, the use of DPPC as a model for understanding the performance of LS is rather limited because its inefficiency in the reservoir formation and its slow respreading at the interface during expansion. This has driven the research on the use of more complex models, including DPPC in combination with other lipids or fatty acids, e.g., palmitic acid, cholesterol or DOPC (1,2-dioleoylsn-glycero-3-phosphocholine), among others [117,136,165,173,[188][189][190][191], which provide a simplified picture of the complex behavior of LS under physiological conditions. The use of these models can provide a deeper understanding of the behavior of LS films because some studies have evidenced the important role of specific lipids, e.g., 1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine (POPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-(phospho-rac-(1glycerol)) (POPG), on the modulation of the phase behavior of LS film [173,189,190,192].…”

Section: Chemistry Of Lung Surfactant Modelsmentioning

confidence: 99%

“…This has driven the research on the use of more complex models, including DPPC in combination with other lipids or fatty acids, e.g., palmitic acid, cholesterol or DOPC (1,2-dioleoylsn-glycero-3-phosphocholine), among others [117,136,165,173,[188][189][190][191], which provide a simplified picture of the complex behavior of LS under physiological conditions. The use of these models can provide a deeper understanding of the behavior of LS films because some studies have evidenced the important role of specific lipids, e.g., 1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine (POPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-(phospho-rac-(1glycerol)) (POPG), on the modulation of the phase behavior of LS film [173,189,190,192]. On the other side, it has been reported that the inclusion of DOPC in LS models provides a suitable environment for gaining information of the remodeling process, contributing to the squeezing-out of lipids and reservoir formation upon compression, and to the respreading of the LS upon expansion [193].…”

Section: Chemistry Of Lung Surfactant Modelsmentioning

confidence: 99%

See 2 more Smart Citations

Fluid Films as Models for Understanding the Impact of Inhaled Particles in Lung Surfactant Layers

Guzmán

2022

Coatings

View full text Add to dashboard Cite

Pollution is currently a public health problem associated with different cardiovascular and respiratory diseases. These are commonly originated as a result of the pollutant transport to the alveolar cavity after their inhalation. Once pollutants enter the alveolar cavity, they are deposited on the lung surfactant (LS) film, altering their mechanical performance which increases the respiratory work and can induce a premature alveolar collapse. Furthermore, the interactions of pollutants with LS can induce the formation of an LS corona decorating the pollutant surface, favoring their penetration into the bloodstream and distribution along different organs. Therefore, it is necessary to understand the most fundamental aspects of the interaction of particulate pollutants with LS to mitigate their effects, and design therapeutic strategies. However, the use of animal models is often invasive, and requires a careful examination of different bioethics aspects. This makes it necessary to design in vitro models mimicking some physico-chemical aspects with relevance for LS performance, which can be done by exploiting the tools provided by the science and technology of interfaces to shed light on the most fundamental physico-chemical bases governing the interaction between LS and particulate matter. This review provides an updated perspective of the use of fluid films of LS models for shedding light on the potential impact of particulate matter in the performance of LS film. It should be noted that even though the used model systems cannot account for some physiological aspects, it is expected that the information contained in this review can contribute on the understanding of the potential toxicological effects of air pollution.

show abstract

Section: Role Of Particle Surface Charge and Wettability On Their Int...mentioning

confidence: 94%

Section: Chemistry Of Lung Surfactant Modelsmentioning

confidence: 99%

Section: Chemistry Of Lung Surfactant Modelsmentioning

confidence: 99%

See 1 more Smart Citation

Fluid Films as Models for Understanding the Impact of Inhaled Particles in Lung Surfactant Layers

Guzmán

2022

Coatings

View full text Add to dashboard Cite

show abstract

“…These values concord with the tilt angles of 12-30° reported for Langmuir monolayers and solid-supported bilayers of dialkylphosphatidylcholines in the condensed or gel phase. [67][68][69][70][71] Overall, the <P2> values and tilt angles mean that the difference in the transition temperatures of the two leaflets observed by AFM is not due to an initial difference in the conformational order or orientation of the acyl chains.…”

Section: Asymmetric Dppc Bilayersmentioning

confidence: 99%

Selective Isotopic Labeling Resolves the Gel-to-Fluid Phase Transitions of the Individual Leaflets of a Planar-Supported Phospholipid Bilayer

et al. 2019

Self Cite

View full text Add to dashboard Cite

Knowledge of the thermotropic phase behavior of solid-supported bilayer lipid assemblies is essential for mimicking the molecular organization and lateral fluidity of cell membranes. The gelto-fluid phase transitions in a homologous series of single phospholipid bilayers supported on planar silicon substrates were investigated by temperature-controlled atomic force microscopy (AFM) and attenuated total reflection infrared (ATR-IR) spectroscopy to obtain complementary information at the mesoscopic and molecular scales. Symmetric bilayers of dipalmitoylphosphatidylcholine (DPPC) and vertically asymmetric bilayers comprised of a leaflet of DPPC and another of acyl-chain-deuterated DPPC (DPPC-d62) were prepared by the Langmuir-Blodgett technique. The selective deuteration of one of the bilayer leaflets enabled the simultaneous monitoring by IR spectroscopy of the acyl chain melting in each leaflet via the spectrally isolated CH2 and CD2 stretching vibrations. Two gel-to-fluid transitions were discerned for both the symmetric and asymmetric bilayers in ultrapure water. The deuterium isotope effect observed in free-standing membranes was maintained for the supported bilayers. IR spectroscopy revealed that the melting of one leaflet promotes the disordering of the acyl chains in the adjacent one. The findings suggest that the two leaflet phase transitions do not evolve in isolation. This work sheds insight into the nature of leaflet-leaflet interactions and the thermodynamic properties of surface-confined phospholipid bilayers.

show abstract

“… 11 , 12 However, the current understanding of the potential biophysical changes associated with particle inhalation is far from clear. On one hand, the interaction of particles with LS alters their surface tension, phase behavior, and interfacial structure, 13 , 14 whereas on the other hand particles are widespread in therapy and diagnostic. 15 , 16 …”

Section: Introductionmentioning

confidence: 99%

Evaluating the Impact of Hydrophobic Silicon Dioxide in the Interfacial Properties of Lung Surfactant Films

Guzmán

Santini

Ferrari

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

The interaction of hydrophobic silicon dioxide particles (fumed silicon dioxide), as model air pollutants, and Langmuir monolayers of a porcine lung surfactant extract has been studied in order to try to shed light on the physicochemical bases underlying the potential adverse effects associated with pollutant inhalation. The surface pressure–area isotherms of lung surfactant (LS) films including increasing amounts of particles revealed that particle incorporation into LS monolayers modifies the organization of the molecules at the water/vapor interface, which alters the mechanical resistance of the interfacial films, hindering the ability of LS layers for reducing the surface tension, and reestablishing the interface upon compression. This influences the normal physiological function of LS as is inferred from the analysis of the response of the Langmuir films upon the incorporation of particles against harmonic changes of the interfacial area (successive compression–expansion cycles). These experiments evidenced that particles alter the relaxation mechanisms of LS films, which may be correlated to a modification of the transport of material within the interface and between the interface and the adjacent fluid during the respiratory cycle.

show abstract

Nanoparticle-induced structural changes in lung surfactant membranes: an X-ray scattering study

Abstract: Low concentrations of cationic silica nanoparticles impact lung surfactant membrane structure while anionic nanoparticles have minimal effect.

Cited by 22 publications

References 60 publications

Fluid Films as Models for Understanding the Impact of Inhaled Particles in Lung Surfactant Layers

Fluid Films as Models for Understanding the Impact of Inhaled Particles in Lung Surfactant Layers

Selective Isotopic Labeling Resolves the Gel-to-Fluid Phase Transitions of the Individual Leaflets of a Planar-Supported Phospholipid Bilayer

Evaluating the Impact of Hydrophobic Silicon Dioxide in the Interfacial Properties of Lung Surfactant Films

Contact Info

Product

Resources

About