Nanoparticle-loaded microcapsules providing effective UV protection for Cry1Ac

Zhang, Yongjing; Aijing, Zhang; Li, Mengyuan; He, Kanglai; Guo, Shuangshuang

doi:10.1080/02652048.2021.1990424

Cited by 5 publications

(5 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, graphene oxide combined with olive oil on the Bt cell mixture showed synergistic UV protection effects, 97 and UV reflective nanomaterials, nano-ZnO, nano-SiO 2 , and nano-TiO 2 , combined with Bt to form a microcapsule formulation with relatively long-lasting UV resistance. 98 Rao et al synthesized nano-Mg(OH) 2 to absorb Cry1Ac protein and control the loss of Cry1Ac protein that can be induced by rainwater flushing, where the nano-Mg(OH) 2 -Cry1Ac mixture can be loaded on the groove of the cotton leaf, which could enhance the anti-rainwater washing ability and insecticidal activity compared to those of the pure protein. 59 Wu et al combined mesoporous silica with Bt Cry5B protein and the mesoporous silica loading not only protected the Cry5B protein from hydrolysis by mammalian pepsin but also improved the protein transmission in the worm and increased the control efficiency of the protein on the nematode.…”

Section: The Application Of Nanotechnology To Insecticidesmentioning

confidence: 99%

See 1 more Smart Citation

Nanobiopesticides in sustainable agriculture: developments, challenges, and perspectives

Pan

Guo

Zhai

et al. 2023

Environ. Sci.: Nano

View full text Add to dashboard Cite

show abstract

Section: The Application Of Nanotechnology To Insecticidesmentioning

confidence: 99%

Section: The Main Categories Of Nanobiopesticides and Their Research ...mentioning

confidence: 99%

Nanobiopesticides in sustainable agriculture: developments, challenges, and perspectives

Pan

Guo

Zhai

et al. 2023

Environ. Sci.: Nano

View full text Add to dashboard Cite

show abstract

“…The S-CNs and SQDs previously prepared were used to synthesize the nanosheets (Cu 2+ -SQDs/S-CN) (Wang et al 2015 ; Zhang et al 2021 ). In summary, 0.1 g of S-CN powder and 1 mL of Cu 2+ -SQDs solution were added to 25 mL ultrapure water, and the mixture was further stirred for 30 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…The major drawback of using Bt proteins as active ingredients in biopesticides is the lack of persistence in nature due to environmental conditions, such as ultraviolet (UV) radiation, high temperatures, and rain, all of which can cause a reduction in the insecticidal activity or can inactivate Cry activity (Leong et al 1980 ; Peralta and Palma 2017 ; Fernández-Chapa et al 2019 ). Indeed, UV light can give rise to the inactivation of crystal proteins by destroying their three-dimensional (3D) structure (Zhang et al 2021 ) and therefore disrupting their solubilization in the alkaline environment of the insect midgut leading to severe repercussions for product stability (Cui et al 1993 ). Also, it is possible that the temperature indirectly accelerated or inhibited the degradation of crystal proteins by indirectly controlling soil microbe activity (Yao-yu et al 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Enhancing insecticidal efficacy of Bacillus thuringiensis Cry1Ab through pH-sensitive encapsulation

Jalali,

Bel,

Maghsoudi

et al. 2023

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Nanotechnology is a promising way to enhance the stability of Bacillus thuringiensis (Bt) insecticidal proteins under environmental conditions. In this work, two emulsions were prepared through the Pickering emulsion technique, stabilized by Cu2+-SQDs/S-CN nanocomposites and by GO nanosheets. In addition, a pH-sensitive polymer was incorporated into these emulsions, allowing the Bt protein, Cry1Ab, to be released in an alkaline pH environment, as it occurs in the lepidopteran pests’ gut. The effectiveness of these two nanomaterials in protecting Cry1Ab from degradation, and therefore enhancing its pesticidal activity, was assessed by exposing samples of the purified unprotected protein and encapsulated protein to high-intensity UV light and 40°C temperature treatments. The UV treatment results were evaluated using SDS-PAGE analysis and pointed out that Cry1Ab could be structurally protected by the emulsions. The bioassays with first instar larvae of the lepidopteran pest Ostrinia nubilalis confirm the nanomaterial protection to UV and temperature treatments, i.e., decreasing about half the degradation rate and increasing up to 12-fold the residual activity after UV treatment. Our results indicate that encapsulation could be an effective strategy to improve the effectiveness of Cry1Ab under environmental conditions. Key points • Pickering emulsions are effective for solubilized Cry1Ab encapsulation. • Structural and toxicity Cry1Ab properties are enhanced by pH-sensitive encapsulation. • Cu2+−SQDs/S-CN and GO nanomaterials improve the efficacy of Bt insecticides.

show abstract

“…The low bioavailability is caused by many factors, including low water solubility and slow dissolution rate, poor permeability, or rapid metabolism and elimination rate (He et al, 2015). Many new dosage forms, such as inclusion complexes (J. Wang et al, 2019), solid dispersions (Tran & Tran, 2020), microcapsules (Zhang et al, 2021), microspheres (Ghosh Dastidar et al, 2018), nanoparticles (Kim et al, 2018), and liposomes (Y. Wang et al, 2022), have been developed to improve the bioavailability. By encapsulating the drugs within these novel carrier systems, an increased elimination half‐life and decreased adverse reactions might be achieved (Bikram & West, 2008).…”

Section: Introductionmentioning

confidence: 99%

Preparation, characterization, and pharmacokinetics of oridonin‐loaded liposomes

Lin

Zhang

et al. 2023

Biomedical Chromatography

View full text Add to dashboard Cite

The aim of this study was to prepare oridonin liposomes and evaluate the physicochemical characteristics and pharmacokinetics in rats. A three-level, three-factor Box-Behnken design was used to optimize the preparation of oridonin liposomes. A highly sensitive high-performance liquid chromatographic quantification method using ultraviolet detection was established and validated for the determination of oridonin in rat plasma. Twelve Sprague-Dawley rats were randomly assigned and injected with 15 mg/kg of oridonin or oridonin liposomes via the tail vein. Pharmacokinetic parameters were estimated using a compartmental modeling approach using PKsolver software. The optimum conditions were as follows: soybean phospholipids/ cholesterol ratio, 3.9:1; soybean phospholipids/drug ratio, 8.5:1; and soybean phospholipid concentration, 1.1%. Under these conditions, the mean particle size, polydispersity index, zeta potential, and encapsulation efficiency of oridonin liposomes were 170.5 nm, 0.246, À30.3 mV, and 76.15%, respectively. The pharmacokinetic results showed that liposomes could significantly prolong the elimination half-life (from 2.88 ± 0.55 to 13.67 ± 3.52 h), increase the area under the concentration-time curve (from 1.65 ± 0.17 to 6.22 ± 0.83 μg h/ml), and decrease the clearance (from 6.62 ± 1.38 to 1.96 ± 0.24 L/kg h). The oridonin liposomes increased the elimination half-life and area under the concentration-time curve and provided a reference for the development of drugs with a short half-life.

show abstract

Nanoparticle-loaded microcapsules providing effective UV protection for Cry1Ac

Cited by 5 publications

References 59 publications

Nanobiopesticides in sustainable agriculture: developments, challenges, and perspectives

Nanobiopesticides in sustainable agriculture: developments, challenges, and perspectives

Enhancing insecticidal efficacy of Bacillus thuringiensis Cry1Ab through pH-sensitive encapsulation

Preparation, characterization, and pharmacokinetics of oridonin‐loaded liposomes

Contact Info

Product

Resources

About